Nafion/polyaniline/silica composite membranes for direct methanol fuel cell application

This work investigates the characterization and performance of polyaniline and silica modified Nafion membranes. The aniline monomers are synthesized in situ to form a polyaniline film, whilst silica is embedded into the Nafion matrix by the polycondensation of tetraethylorthosilicate. The physicochemical properties are studied by means of X-ray diffraction and Fourier transform infrared techniques and show that the polyaniline layer is formed on the Nafion surface and improves the structural properties of Nafion in methanol solution. Nafion loses its crystallinity once exposed to water and ethanol, whilst the polyaniline modification allows crystallinity to be maintained under similar conditions. By contrast, the proton conductivities of polyaniline modified membranes are 3–5-fold lower than that of Nafion. On a positive note, methanol crossover is reduced by over two orders of magnitude, as verified by crossover limiting current analysis. The polyaniline modification allows the membrane to become less hydrophilic, which explains the lower proton conductivity. No major advantages are observed by embedding silica into the Nafion matrix. The performance of a membrane electrode assembly (MEA) using commercial catalysts and polyaniline modified membranes in a cell gives a peak power of 8 mW cm⁻² at 20 °C with 2 M methanol and air feeding. This performance correlates to half that of MEAs using Nafion, though the membrane modification leads to a robust material that may allow operation at high methanol concentration.     

On the second order topological asymptotic expansion

The topological derivative provides the sensitivity of a given shape functional with respect to an infinitesimal (non smooth) domain perturbation at an arbitrary point of the domain. Classically, this derivative comes from the second term of the topological asymptotic expansion, dealing only with infinitesimal perturbations. However, for practical applications, we need to insert perturbations of finite size. Therefore, we consider one more term in the expansion which is defined as the second order topological derivative. In order to present these ideas, in this work we calculate first as well as second order topological derivatives for the total potential energy associated to the Laplace’s equation, when the domain is perturbed with a hole. Furthermore, we also study the effects of different boundary conditions on the hole: Neumann and Dirichlet (both homogeneous). In the Neumann’s case, the second order topological derivative depends explicitly on higher-order gradients of the state solution and also implicitly on the point where the hole is nucleated through the solution of an auxiliary problem. On the other hand, in the Dirichlet’s case, the first order topological derivative depends explicitly on the state solution as well as implicitly through the solution of an auxiliary problem, and the second order topological derivative depends only explicitly on the solution associated to the original problem. Finally, we present two simple examples showing the influence of both terms in the second order topological asymptotic expansion for each case of boundary condition on the hole.     

Soy protein containing isoflavones favorably influences macrophage lipoprotein metabolism but not the development of atherosclerosis in CETP transgenic mice

The possibility that soy protein containing isoflavones influences the development of experimental atherosclerosis has been investigated in ovariectomized mice heterozygous for the human CETP transgene and for the LDL-receptor null allele (LDLr^+/− CETP^+/−). After ovariectomy at 8 wk of age they were fed a fat/cholesterol-rich diet for 19 wk and divided into three experimental groups: dietary unmodified soy protein containing isoflavones (mg/g of diet), either at low-dose (Iso Low, 0.272, n=25), or at high-dose (Iso High, 0.535, n=28); and the atherogenic diet containing an isoflavone-depleted alcohol-washed soy protein as a control group (n=28). Aortic root lipid-stained lesion area (mean μm²×10³±SD) did not differ among Iso Low (12.3±9.9), Iso High (7.4±6.4), and controls (10.7±12.8). Autoantibody titers against plasma oxidized LDL did not differ among the experimental groups. Using the control mice as the reference value (100%), in vitro mouse peritoneal macrophage uptake of labeled acetylated LDL-cholesterol was lower in the Iso High (68%) than in the Iso Low (85%) group. The in vitro percent removal by exogenous HDL of labeled unesterified cholesterol from macrophages previously enriched with human [4-¹⁴C]-cholesteryl oleate acetylated LDL was enhanced in the Iso High group (50%). In spite of these in vitro potentially antiatherogenic actions, soy protein containing isoflavones did not modify the average size of lipid-stained area in the aortic root.     

Electronic Structure and Half-Metallic Character in Sr2CoB′O6 (B′ = Mo, Re) Materials

We report several ab initio calculations performed for Sr₂CoB′O₆ (B′ = Mo, Re) by means of the Density Functional Theory and the Linearized Augmented Plane Waves method with spin polarization. For the calculations, the exchange and correlation potentials were included through the local density LDA+U approximation with B3PW91. Density of states (DOS) study was carried out considering both up and down spin polarizations by the Generalized Gradient Approximation (GGA). From the Murnaghan state equation, we calculate the cell dimensions that minimize the total energy. Our results of DOS calculations show that the Sr₂CoMoO₆ material presents a metallic behavior, while Sr₂CoReO₆ reveals a half-metallic nature with conductor behavior for the spin down orientation and semiconducting feature for spin up channel. It was observed close to Fermi level that the low-energy spin down states of Co are responsible by the majority contribution to conduction band. The calculated effective cell magnetic moment of the Sr₂CoReO₆ compound evidences a value 2.02 μ _B , which is close to an integer number as expected for a half-metallic material.     

Nanostructured metal oxides obtained by means polymerization-combustion at low temperature for CO selective oxidation

We investigated the synthesis of nanosized Co₃O₄, NiO and CuO oxides by the polymerization-combustion method and evaluated in the selective oxidation of CO. These materials were characterized before and after catalytic test under specific conditions by X-ray diffraction, scanning electron microscopy, temperature programmed reduction, Raman spectroscopy and Diffuse reflectance infrared Fourier transform spectroscopy. For isoconversion the activity follows the order: Co₃O₄>NiO > CuO. The intrinsic activities under isothermal conditions at 100 °C are: 0.20 × 10⁻³ s⁻¹ for the Co₃O₄ and 0.08 × 10⁻³ s⁻¹ for NiO and CuO. Therefore, the activity of the Co₃O₄, is 3 times higher than for NiO and CuO. The XRD and Raman analyses after catalytic test confirm the existence of a partial reduction of oxides to the metallic phase and no evidence of carbon deposition over surface materials. In the case of nickel oxide the analyzed sample shows the metallic phase of nickel and in the case of copper oxide the sample presents a high grade of reduction. XRD results confirm the presence of Cu₂O and metallic Cu. Finally, results showed that these oxide catalysts present a high resistance to carbonaceous formation under present reaction conditions and confirm the effectiveness of polymerization-combustion technique for synthesis of active high catalyst in the selective oxidation of CO.     

Nutritional behavior of cyclists during a 24-hour team relay race: a field study report

Background  

Information about behavior of energy intake in ultra-endurance cyclists during a 24-hour team relay race is scarce. The nutritional strategy during such an event is an important factor which athletes should plan carefully before the race. The purpose of this study was to examine and compare the nutritional intake of ultra-endurance cyclists during a 24-hour team relay race with the current nutritional guidelines for endurance events. Additionally, we analyzed the relationship among the nutritional and performance variables.     

Proving the efficiency of template matching-based markerless tracking methods which consider the camera perspective deformations

Template matching techniques are often used for camera tracking. They provide a good balance between computational cost and robustness to illumination changes. However, they lack robustness to camera orientation and scale changes. Camera movement, and specially rotation, generates perspective deformations that affect the process of patch matching, so the number of inliers (3D–2D correspondences) decreases. This fact affects camera tracking stability. This paper provides the following statistical proof: considering surface normals associated with 3D points substantially increases the number of inliers. So, this paper shows that computing perspective compensation improves the tracking. For instance, in a particular camera path used for experiments in this paper, without compensation, only a \(14\%\) of 3D points projected into the image were found as inliers, while perspective compensation increased that figure up to a \(65\%\). These results must be contextualized in the analysis provided by the paper.     

Solution methods for the double-support indeterminacy in human gait

There is a growing interest in predicting the gait motion of real subjects under virtual conditions, e.g., to anticipate the result of surgery or to help in the design of prosthetic/orthotic devices. To this end, the motion parameters can be considered as the design parameters of an optimization problem. In this context, determination of the joint efforts for a given motion is a required step for the subsequent evaluation of cost function and constraints. In the double-support phase of gait, the ground reaction forces include twelve unknowns, rendering the inverse dynamics problem indeterminate if no force plate data are available. In this paper, several methods for solving the inverse dynamics problem of the human gait during the double-support phase, using force plates or not, are presented and compared.     

Immunomodulating capacity of kefir

Kefir is a fermented milk produced by the action of lactic acid bacteria, yeasts and acetic acid bacteria, trapped in a complex matrix of polysaccharides and proteins. Beyond its inherent high nutritional value as a source of proteins and calcium, kefir has a long tradition of being regarded as good for health in countries where it is a staple in the diet. However, published human or animal feeding trials to substantiate this view are not numerous. The aim of this work was to determine the immunomodulating capacity of kefir on the intestinal mucosal immune response in mice and to demonstrate the importance of dose and cell viability on this response. BALB/c mice were fed with commercial kefir ad libitum (diluted 1/10, 1/50, 1/100 or 1/200) or pasteurized kefir (diluted 1/6, 1/10, 1/50, 1/100) for 2, 5 or 7 consecutive days. At the end of each feeding period, the bacterial translocation assay was performed in the liver. Small intestine structure was studied by haematoxilin-eosin staining and light microscopy. The number of IgA+ and IgG+ cells was also determined. For the functional doses chosen, cytokines (IL-2, IL-4, IL-6, IL-10, IL-12, TNF-alpha and IFN-gamma) were determined. Kefir and pasteurized kefir were able to modulate the mucosal immune system in a dose-dependent manner. Kefir was administred 10-times more diluted than pasteurized kefir, but it induced an immunomodulation of similar magnitude, indicating the importance of cell viabilty. The results suggest that a Th1 response was controlled by Th2 cytokines induced by kefir feeding. Pasteurized kefir would induce both Th2 and Th1 responses. This is the first study in vivo regarding the mechanisms involved in the immunomodulating capacity of the oral administration of kefir containing viable or heat-inactivated bacteria at different doses.