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排序方式: 共有1718条查询结果,搜索用时 203 毫秒
41.
The aim of this paper is to provide a composite likelihood approach to handle spatially correlated survival data using pairwise joint distributions. With e-commerce data, a recent question of interest in marketing research has been to describe spatially clustered purchasing behavior and to assess whether geographic distance is the appropriate metric to describe purchasing dependence. We present a model for the dependence structure of time-to-event data subject to spatial dependence to characterize purchasing behavior from the motivating example from e-commerce data. We assume the Farlie-Gumbel-Morgenstern (FGM) distribution and then model the dependence parameter as a function of geographic and demographic pairwise distances. For estimation of the dependence parameters, we present pairwise composite likelihood equations. We prove that the resulting estimators exhibit key properties of consistency and asymptotic normality under certain regularity conditions in the increasing-domain framework of spatial asymptotic theory. 相似文献
42.
Automated segmentation of blood vessels in retinal images can help ophthalmologists screen larger populations for vessel abnormalities. However, automated vessel extraction is difficult due to the fact that the width of retinal vessels can vary from very large to very small, and that the local contrast of vessels is unstable. Further, the small vessels are overwhelmed by Gaussian-like noises. Therefore the accurate segmentation and width estimation of small vessels are very challenging. In this paper, we propose a simple and efficient multiscale vessel extraction scheme by multiplying the responses of matched filters at three scales. Since the vessel structures will have relatively strong responses to the matched filters at different scales but the background noises will not, scale production could further enhance vessels while suppressing noise. After appropriate selection of scale parameters and appropriate normalization of filter responses, the filter responses are then extracted and fused in the scale production domain. The experimental results demonstrate that the proposed method works well for accurately segmenting vessels with good width estimation. 相似文献
43.
Directed evolution for the development of conformation-specific affinity reagents using yeast display 总被引:1,自引:0,他引:1
Weaver-Feldhaus JM Miller KD Feldhaus MJ Siegel RW 《Protein engineering, design & selection : PEDS》2005,18(11):527-536
Yeast display is a powerful tool for increasing the affinity and thermal stability of scFv antibodies through directed evolution. Mammalian calmodulin (CaM) is a highly conserved signaling protein that undergoes structural changes upon Ca(2+) binding. In an attempt to generate conformation-specific antibodies for proteomic applications, a selection against CaM was undertaken. Flow cytometry-based screening strategies to isolate easily scFv recognizing CaM in either the Ca(2+)-bound (Ca(2+)-CaM) or Ca(2+)-free (apo-CaM) states are presented. Both full-length scFv and single-domain VH only clones were isolated. One scFv clone having very high affinity (K(d) = 0.8 nM) and specificity (>1000-fold) for Ca(2+)-CaM was obtained from de novo selections. Subsequent directed evolution allowed the development of antibodies with higher affinity (K(d) = 1 nM) and specificity (>300-fold) for apo-CaM from a parental single-domain clone with both a modest affinity and specificity for that particular isoform. CaM-binding activity was unexpectedly lost upon conversion of both conformation-specific clones into soluble fragments. However, these results demonstrate that conformation-specific antibodies can be quickly and easily isolated by directed evolution using the yeast display platform. 相似文献
44.
Rotheram-Borus Mary Jane; Weiss Robert; Alber Susan; Lester Patricia 《Canadian Metallurgical Quarterly》2005,73(2):221
The impact of HIV-related parental death on 414 adolescents was examined over a period of 6 years. The adjustment of bereaved adolescents was compared over 4 time periods relative to parental death and was also compared with the adjustment of nonbereaved adolescents. Bereaved adolescents had significantly more emotional distress, negative life events, and contact with the criminal justice system than nonbereaved youths; these behaviors did not remain significantly higher after parental death. Depressive symptoms and passive problem solving increased soon after parental death, as compared with nonbereaved adolescents. One year subsequent to parental death, depression and passive problem solving were similar to the levels of nonbereaved peers. Only sexual risk behaviors increased following parental death. These results suggest the importance of early family intervention soon after parental HIV diagnosis, prior to parental death, and sustained over time. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
45.
Visual search has been studied extensively, yet little is known about how its constituent processes affect subsequent emotional evaluation of searched-for and searched-through items. In 3 experiments, the authors asked observers to locate a colored pattern or tinted face in an array of other patterns or faces. Shortly thereafter, either the target or a distractor was rated on an emotional scale (patterns, cheerfulness; faces, trustworthiness). In general, distractors were rated more negatively than targets. Moreover, distractors presented near the target during search were rated significantly more negatively than those presented far from the target. Target-distractor proximity affected distractor ratings following both simple-feature and difficult-conjunction search, even when items appeared at different locations during evaluation than during search and when faces previously tinted during search were presented in grayscale at evaluation. An attentional inhibition account is offered to explain these effects of attention on emotional evaluation. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
46.
47.
Helenice Jane Cote Gil Coury 《Applied ergonomics》1998,29(6):17
The objective of this study was to evaluate an auto-instructional preventive programme designed to allow the subjects to identify critical aspects related to their work and musculoskeletal comfort, and provide them with some simple alternatives for controlling the identified problems. The programme was evaluated by 36 secretaries and bank clerks through their symptom perception before and after the programme. The results showed that the symptoms increased in number and severity. The programme seemed to have helped the participants to become aware of the ergonomic problems present in their job and to link these problems to physical symptoms. Self-administered preventive programmes can lead to clearly negative results when applied as the only measure to control musculoskeletal symptoms. 相似文献
48.
理论分析表明在镀有金属分光膜的玻璃介质和金属介质构成的几何楔形成的斐索干涉条纹中,相长干涉并不位于相邻的两相消干涉的中央,而是存在一明显偏移,这一偏移与引入几何楔中待测液体的折射率相关.基于该原理,提出了一种新的折射率测量的方法,并对该方进行了实验验证.该方法不需要利用标准液对光学几何量进行标定,测量范围广. 相似文献
49.
Sadek Joseph R.; Johnson Shannon A.; White Desirée A.; Salmon David P.; Taylor Kirsten I.; DeLaPena Jody H.; Paulsen Jane S.; Heaton Robert K.; Grant Igor 《Canadian Metallurgical Quarterly》2004,18(4):692
Remote memory was assessed in persons with HIV-associated dementia (HIV-D), probable Alzheimer's disease (AD), and Huntington's disease (HD) and in healthy controls. The clinical groups were similar in overall dementia severity. Each clinical group exhibited impairments on remote memory tests relative to controls; however, temporally graded memory loss with selective preservation of older information was observed in the AD group but not the HD or HIV-D group. Analysis of cued retrieval indicated a preferential cuing benefit for the HIV-D and HD groups relative to the AD group. The similar pattern of remote memory performance demonstrated by the HIV-D and HD groups is a novel finding and suggests a subcortically mediated retrograde amnesia in HIV-D. The temporally graded pattern and the abnormal cued retrieval performance in the AD group are consistent with a consolidation deficit associated with extrahippocampal (cortical) and hippocampal damage. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
50.