Properties,Physiological Functions and Involvement of Basidiomycetous Alcohol Oxidase in Wood Degradation

Extensive research efforts have been devoted to describing yeast alcohol oxidase (AO) and its promoter region, which is vastly applied in studies of heterologous gene expression. However, little is known about basidiomycetous AO and its physiological role in wood degradation. This review describes several alcohol oxidases from both white and brown rot fungi, highlighting their physicochemical and kinetic properties. Moreover, the review presents a detailed analysis of available AO-encoding gene promoter regions in basidiomycetous fungi with a discussion of the manipulations of culture conditions in relation to the modification of alcohol oxidase gene expression and changes in enzyme production. The analysis of reactions catalyzed by lignin-modifying enzymes (LME) and certain lignin auxiliary enzymes (LDA) elucidated the possible involvement of alcohol oxidase in the degradation of derivatives of this polymer. Combined data on lignin degradation pathways suggest that basidiomycetous AO is important in secondary reactions during lignin decomposition by wood degrading fungi. With numerous alcoholic substrates, the enzyme is probably engaged in a variety of catalytic reactions leading to the detoxification of compounds produced in lignin degradation processes and their utilization as a carbon source by fungal mycelium.     

Computational Pipeline for Reference-Free Comparative Analysis of RNA 3D Structures Applied to SARS-CoV-2 UTR Models

RNA is a unique biomolecule that is involved in a variety of fundamental biological functions, all of which depend solely on its structure and dynamics. Since the experimental determination of crystal RNA structures is laborious, computational 3D structure prediction methods are experiencing an ongoing and thriving development. Such methods can lead to many models; thus, it is necessary to build comparisons and extract common structural motifs for further medical or biological studies. Here, we introduce a computational pipeline dedicated to reference-free high-throughput comparative analysis of 3D RNA structures. We show its application in the RNA-Puzzles challenge, in which five participating groups attempted to predict the three-dimensional structures of 5′- and 3′-untranslated regions (UTRs) of the SARS-CoV-2 genome. We report the results of this puzzle and discuss the structural motifs obtained from the analysis. All simulated models and tools incorporated into the pipeline are open to scientific and academic use.     

River, reservoir and lake sediment contamination by heavy metals downstream from urban areas of Switzerland

This paper quantifies sediment contamination by heavy metals in four reservoirs and one lake located downstream from major urban areas in Switzerland. The waterbodies include the Wettingen Reservoir (located on the Limmat River downstream from Zürich), the Klingnau Reservoir (on the lower Aare River), the Wohlen Reservoir (downstream from Berne), the Verbois Reservoir (downstream from Geneva) and Vidy Bay (Lake Geneva, city of Lausanne). For all sediment cores and contaminants, a trend is observed from high contaminant values in the lower part of the cores, decreasing to lower concentrations in the upper part of the cores. However, for each site and each element, specific features are recognized. Applying the criteria of the Swiss ordinance on soil protection, all sediment cores must be classified as contaminated by one or more contaminants and at variable levels. From these data, it is concluded that: reservoirs and lakes located downstream from major urban centres in Switzerland have accumulated significant volumes of contaminated sediments in the past, representing the largest, but not the most intensely, contaminated sites on a national scale; the main environmental risk is remobilization of the contaminants and their return to the food chain, particularly by infiltration into the groundwater; and although the processes of remobilization are identified, the conditions of occurrence and the amplitude of the processes are still poorly known. Different options of reservoir and lake sediment management also are discussed and further research topics defined.     

A new method of determination of the permeability of vapors of volatile liquid substances through polymer membranes

A new apparatus for the determination of vapor permeability of volatile liquid substances through polymer membranes has been described. The permeability of the vapor is calculated from the measurements of the depression rate of the liquid meniscus in a calibrated capillary tube. The conditions of the measurements by means of the described method are very close to those of the average and practical application of foils. The method is characterized by a simple design of the apparatus, easy handling, and simplicity of the determinations, so it may constitute the basis of vapor permeability determination standards. The results obtained by means of the described method were compared with those derived from the partition cell method.     

Dilatometric studies of the phase transitions of sodium potassium tartrate tetrahydrate (RS)

Temperature dependence of the thermal expansion coefficients of Rochelle salt (RS) crystals for the basic crystallographic directions have been measured in the temperature range from 100 K to 308 K by an air-capacitor dilatometer. Anomalies of thermal expansion coefficients at about 210 K and 153 K have been observed, besides these corresponding to known ferroelectric phase transitions at the temperatures of 255 and 297 K.     

Kalman filtering of traffic fluctuations for real-time network management

Discrete- time stochastic models of sequential traffic records in telephone networks are considered. They appear to be well suited for a use of the Kaiman filter. A version of the filtering algorithm is adopted to deal with traffic fluctuations. The overall approach is tested against the real data gathered by the supervisor of the French toll network. The adaptive traffic estimator is inserted into a simulator of a testbed network operated in the urban zone of Paris. The traffic estimations provided by the filter are used to decide an overflow hierarchy.     

General Probability of System Failure

This paper presents a general method to determine probabilities of failure of any fixed subset of coherent system components under various conditions. The method uses a known reliability structure of the system and the known joint probability distribution of its component times-to-failure. This method is universal and can be applied in many cases. Nevertheless, for large systems it is troublesome. In practice a problem is solved using a numerical program.     

Apparatus for real-time measurement of particle size distribution

In this paper we describe an apparatus for real-time measurement of particle size distribution. By introducing a fast moving water stream with suspended particles and stroboscopic illumination, a large number of pictures (samples) can be analysed by means of a TV camera connected to a computer. In this manner complete particle size distribution is achieved with minimal delay. Satisfactory results were obtained when the apparatus was tested as an on-line device in a flotation plant, demonstrating the full ability of the system to control the flotation process.     

Mathematical foundations of the quasistatic approximation in reactor physics

The quasistatic method used in reactor-physics calculations is applied to a model integro-differential equation in a Banach space. The resulting quasistatic equations are shown to be locally consistent with the original initial-value problem. An iterative scheme to solve these equations is presented and shown to be convergent.