Emmanuel de Jesús Ramírez-Rivera  Ingrid Mayanin Rodriguez-Buenfil  Mirielen Pérez-Robles  Julio Enrique Oney-Montalvo  Witoon Prinyawiwatkul  Adán Cabal-Prieto  Manuel Octavio Ramírez-Sucre  José Andrés Herrera-Corredor 《International Journal of Food Science & Technology》2021,56(2):573-586

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Konrad Skotnicki  Slawomir Ostrowski  Jan Cz. Dobrowolski  Julio R. De la Fuente  Alvaro Caete  Krzysztof Bobrowski 《International journal of molecular sciences》2021,22(2)

The azide radical (N₃^●) is one of the most important one-electron oxidants used extensively in radiation chemistry studies involving molecules of biological significance. Generally, it was assumed that N₃^● reacts in aqueous solutions only by electron transfer. However, there were several reports indicating the possibility of N₃^● addition in aqueous solutions to organic compounds containing double bonds. The main purpose of this study was to find an experimental approach that allows a clear assignment of the nature of obtained products either to its one-electron oxidation or its addition products. Radiolysis of water provides a convenient source of one-electron oxidizing radicals characterized by a very broad range of reduction potentials. Two inorganic radicals (SO₄^●−, CO₃^●−) and Tl²⁺ ions with the reduction potentials higher, and one radical (SCN)₂^●− with the reduction potential slightly lower than the reduction potential of N₃^● were selected as dominant electron-acceptors. Transient absorption spectra formed in their reactions with a series of quinoxalin-2-one derivatives were confronted with absorption spectra formed from reactions of N₃^● with the same series of compounds. Cases, in which the absorption spectra formed in reactions involving N₃^● differ from the absorption spectra formed in the reactions involving other one-electron oxidants, strongly indicate that N₃^● is involved in the other reaction channel such as addition to double bonds. Moreover, it was shown that high-rate constants of reactions of N₃^● with quinoxalin-2-ones do not ultimately prove that they are electron transfer reactions. The optimized structures of the radical cations (7-R-3-MeQ)^●+, radicals (7-R-3-MeQ)^● and N₃^● adducts at the C2 carbon atom in pyrazine moiety and their absorption spectra are reasonably well reproduced by density functional theory quantum mechanics calculations employing the ωB97XD functional combined with the Dunning’s aug-cc-pVTZ correlation-consistent polarized basis sets augmented with diffuse functions.  相似文献   

    

Yulan Gong  Rajeswari Nagarathinam  Maria F. Arisi  Lorenzo Gerratana  Jennifer S. Winn  Michael Slifker  Jianming Pei  Kathy Q. Cai  Zachary Hasse  Elias Obeid  Julio Noriega  Christopher Sebastiano  Eric Ross  Katherine Alpaugh  Massimo Cristofanilli  Sandra V. Fernandez 《International journal of molecular sciences》2021,22(16)

To better understand the etiology of inflammatory breast cancer (IBC) and identify potential therapies, we studied genomic alterations in IBC patients. Targeted, next-generation sequencing (NGS) was performed on cell-free DNA (cfDNA) (n = 33) and paired DNA from tumor tissues (n = 29) from 32 IBC patients. We confirmed complementarity between cfDNA and tumor tissue genetic profiles. We found a high incidence of germline variants in IBC patients that could be associated with an increased risk of developing the disease. Furthermore, 31% of IBC patients showed deficiencies in the homologous recombination repair (HRR) pathway (BRCA1, BRCA2, PALB2, RAD51C, ATM, BARD1) making them sensitive to poly (ADP-ribose) polymerase (PARP) inhibitors. We also characterized the tumor-infiltrating lymphocytes (TILs) in tumor tissue biopsies by studying several markers (CD4, CD8, FoxP3, CD20, PD-1, and PD-L1) through immunohistochemistry (IHC) staining. In 7 of 24 (29%) patients, tumor biopsies were positive for PD-L1 and PD-1 expression on TILs, making them sensitive to PD-1/PD-L1 blocking therapies. Our results provide a rationale for considering PARP inhibitors and PD-1/PDL1 blocking immunotherapy in qualifying IBC patients.  相似文献   

    

Vanesa Palau  Sofia Villanueva  Josu Jarrín  David Benito  Eva Mrquez  Eva Rodríguez  María Jos Soler  Anna Oliveras  Javier Gimeno  Laia Sans  Marta Crespo  Julio Pascual  Clara Barrios  Marta Riera 《International journal of molecular sciences》2021,22(23)

Acute and chronic kidney lesions induce an increase in A Disintegrin And Metalloproteinase domain 17 (ADAM17) that cleaves several transmembrane proteins related to inflammatory and fibrotic pathways. Our group has demonstrated that renal ADAM17 is upregulated in diabetic mice and its inhibition decreases renal inflammation and fibrosis. The purpose of the present study was to analyze how Adam17 deletion in proximal tubules affects different renal structures in an obese mice model. Tubular Adam17 knockout male mice and their controls were fed a high-fat diet (HFD) for 22 weeks. Glucose tolerance, urinary albumin-to-creatinine ratio, renal histology, and pro-inflammatory and pro-fibrotic markers were evaluated. Results showed that wild-type mice fed an HFD became obese with glucose intolerance and renal histological alterations mimicking a pre-diabetic condition; consequently, greater glomerular size and mesangial expansion were observed. Adam17 tubular deletion improved glucose tolerance and protected animals against glomerular injury and prevented podocyte loss in HFD mice. In addition, HFD mice showed more glomerular macrophages and collagen accumulation, which was prevented by Adam17 deletion. Galectin-3 expression increased in the proximal tubules and glomeruli of HFD mice and ameliorated with Adam17 deletion. In conclusion, Adam17 in proximal tubules influences glucose tolerance and participates in the kidney injury in an obese pre-diabetic murine model. The role of ADAM17 in the tubule impacts on glomerular inflammation and fibrosis.  相似文献   

    

Angelica Judith Granados-Lpez  Eduardo Manzanares-Acua  Yamil Lpez-Hernndez  Julio Enrique Castaeda-Delgado  Ixamail Fraire-Soto  Claudia Araceli Reyes-Estrada  Rosalinda Gutirrez-Hernndez  Jesús Adrin Lpez 《International journal of molecular sciences》2021,22(10)

Ultraviolet (UV) exposure has been linked to skin damage and carcinogenesis, but recently UVB has been proposed as a therapeutic approach for cancer. Herein, we investigated the cellular and molecular effects of UVB in immortal and tumorigenic HPV positive and negative cells. Cells were irradiated with 220.5 to 1102.5 J/m² of UVB and cell proliferation was evaluated by crystal violet, while cell cycle arrest and apoptosis analysis were performed through flow cytometry. UVB effect on cells was recorded at 661.5 J/m² and it was exacerbated at 1102.5 J/m². All cell lines were affected by proliferation inhibition, cell cycle ablation and apoptosis induction, with different degrees depending on tumorigenesis level or HPV type. Analysis of the well-known UV-responsive p53, E2F1 and microtubules system proteins was performed in SiHa cells in response to UVB through Western-blotting assays. E2F1 and the Microtubule-associated protein 2 (MAP2) expression decrease correlated with cellular processes alteration while p53 and Microtubule-associated Protein 1S (MAP1S) expression switch was observed since 882 J/m², suggesting they were required under more severe cellular damage. However, expression transition of α-Tubulin3C and β-Tubulin was abruptly noticed until 1102.5 J/m² and particularly, γ-Tubulin protein expression remained without alteration. This study provides insights into the effect of UVB in cervical cancer cell lines.  相似文献   

    

Teresa Peir  Miriam Alonso-Carpio  Pilar Ribera  Patricia Almudver  Ins Roger  Paula Montero  Severiano Marín  Javier Milara  Julio Cortijo 《International journal of molecular sciences》2022,23(23)

Skin fibrosis is a hallmark of a wide array of dermatological diseases which can greatly impact the patients’ quality of life. Galectin-3 (GAL-3) has emerged as a central regulator of tissue fibrosis, playing an important pro-fibrotic role in numerous organs. Various studies are highlighting its importance as a skin fibrotic diseases biomarker; however, there is a need for further studies that clarify its role. This paper aims to ascertain whether the expression of GAL-3 is increased in relevant in vitro and in vivo models of skin fibrosis. We studied the role of GAL-3 in vitro using normal human dermal fibroblasts (NHDF) and fibrocytes. In addition, we used a skin fibrosis murine model (BALB/c mice) and human biopsies of healthy or keloid tissue. GAL-3 expression was analyzed using real time PCR, Western blot and immunostaining techniques. We report a significantly increased expression of GAL-3 in NHDF and fibrocytes cell cultures following stimulation with transforming growth factor β1 (TGFβ1). In vivo, GAL-3 expression was increased in a murine model of systemic sclerosis and in human keloid biopsies. In sum, this study underlines the involvement of GAL-3 in skin fibrosis using several models of the disease and highlights its role as a relevant target.  相似文献   

    

Jernimo Aragn-Vela  Jesús Alcal-Bejarano Carrillo  Aurora Moreno-Racero  Julio Plaza-Diaz 《International journal of molecular sciences》2022,23(23)

Obesity and overweight are defined as abnormal fat accumulations. Adipose tissue consists of more than merely adipocytes; each adipocyte is closely coupled with the extracellular matrix. Adipose tissue stores excess energy through expansion. Obesity is caused by the abnormal expansion of adipose tissue as a result of adipocyte hypertrophy and hyperplasia. The process of obesity is controlled by several molecules, such as integrins, kindlins, or matrix metalloproteinases. In children with obesity, metabolomics studies have provided insight into the existence of unique metabolic profiles. As a result of low-grade inflammation in the system, abnormalities were observed in several metabolites associated with lipid, carbohydrate, and amino acid pathways. In addition, obesity and related hormones, such as leptin, play an instrumental role in regulating food intake and contributing to childhood obesity. The World Health Organization states that physical activity benefits the heart, the body, and the mind. Several noncommunicable diseases, such as cardiovascular disease, cancer, and diabetes, can be prevented and managed through physical activity. In this work, we reviewed pediatric studies that examined the molecular and hormonal control of obesity and the influence of physical activity on children with obesity or overweight. The purpose of this review was to examine some orchestrators involved in this disease and how they are related to pediatric populations. A larger number of randomized clinical trials with larger sample sizes and long-term studies could lead to the discovery of new key molecules as well as the detection of significant factors in the coming years. In order to improve the health of the pediatric population, omics analyses and machine learning techniques can be combined in order to improve treatment decisions.  相似文献   

    

Edgar A. Martínez Londoo  Julio E. Can Barriga  Matilda Palm 《Biofuels, Bioproducts and Biorefining》2016,10(4):359-368

Building on an extensive literature review that included peer‐reviewed publications, grey literature, national statistics, official reports, and regulations enacted by the Colombian government, this paper identifies opportunities, challenges, and constraints faced by solid biofuels production from energy crops in Colombia. Our findings suggest that the solid biofuels industry currently lacks policy regulations and an adequate research framework. The paper notes the industry's market potential and addresses its dependency on a legal framework, political willingness, and technological developments. The legal framework includes land ownership formalization, job regulations, and the definition of environmental and administrative permits. Political willingness relates to governmental policies and financial incentives based on environmental and sustainability criteria, which can make the sector competitive compared to other energy sources at the local and international market scales. The technological aspects include public and private support for research and development programs and a strategic analysis of industry‐specific requirements for infrastructure, conversion, and transportation within a life cycle assessment framework. The preliminary land‐use analysis suggests the potential availability of land for solid biofuels production in the Caribbean, Andean, Inter‐Andean Valley, and Orinoco regions. Furthermore, the results show that solid biofuels production can potentially supply internal demand and play a role in international markets with strategic development and government support. © 2016 Society of Chemical Industry and John Wiley & Sons, Ltd  相似文献   

    

Julio Beltrán Ortega  Diego M Martínez Gila  Daniel Aguilera Puerto  Javier Gámez García  Juan Gómez Ortega 《Journal of the science of food and agriculture》2016,96(14):4644-4662

The quality of virgin olive oil is related to the agronomic conditions of the olive fruits and the process variables of the production process. Nowadays, food markets demand better products in terms of safety, health and organoleptic properties with competitive prices. Innovative techniques for process control, inspection and classification have been developed in order to to achieve these requirements. This paper presents a review of the most significant sensing technologies which are increasingly used in the olive oil industry to supervise and control the virgin olive oil production process. Throughout the present work, the main research studies in the literature that employ non‐invasive technologies such as infrared spectroscopy, computer vision, machine olfaction technology, electronic tongues and dielectric spectroscopy are analysed and their main results and conclusions are presented. These technologies are used on olive fruit, olive slurry and olive oil to determine parameters such as acidity, peroxide indexes, ripening indexes, organoleptic properties and minor components, among others. © 2016 Society of Chemical Industry  相似文献   

    

Juan Garrido  Francisco Vázquez  Fernando Morilla  Julio E. Normey-Rico 《International journal of systems science》2016,47(2):374-388

This paper presents a new methodology to design multivariable Smith predictor for n×n processes with multiple time delays based on the centralised inverted decoupling structure. The controller elements are calculated in order to achieve good reference tracking and decoupling response. Independent of the system size, very simple general expressions for the controller elements are obtained. The realisability conditions are provided and the particular case of processes with all of its elements as first-order plus time delay systems is discussed in more detail. A diagonal filter is added to the proposed control structure in order to improve the disturbance rejection without modifying the nominal set-point response and to obtain a stable output prediction in unstable plants. The effectiveness of the method is illustrated through different simulation examples in comparison with other works.  相似文献