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121.
A review of optical, chemical, and biological sensors to detect metabolic activity at the single-cell level is presented in the context of the development of lab-on-a-chip research instrumentation. The sensors reviewed include optical sensors, at both research and commercial levels, that can optically detect intracellular metabolites including adenosine triphosphate, nicotinamide-adenine dinucleotide, reduced flavin adenine dinucleotide, and other metabolites, including oxygen, carbon dioxide, and glucose. Methods to optically detect pH changes which are a general indicator of activity in extracellular space are also briefly reviewed. Performance metrics such as sensitivity, sensor size, drift, time response, and sensing range are included when available. Highly suitable optical sensor technologies for monitoring cellular metabolic activity include luminescent (fluorescent, phosphorescent, and chemiluminescent) and colorimetric optical probes. Different approaches to extracting luminescent and colorimetric information are reviewed, including benchtop techniques, fiber-optic approaches, and the use of probes encapsulated by biologically localized embedding. A brief discussion of alternate optical sensor technologies, such as surface plasmon resonance and infrared absorption spectroscopy, is also presented. 相似文献
122.
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124.
M. Ritchie P. D. Lee A. Mitchell S. L. Cockcroft T. Wang 《Metallurgical and Materials Transactions A》2003,34(3):863-877
An energy dispersive X-ray (EDX) detector mounted on a laboratory scale electron beam furnace (30 kW) was employed to assess
the potential use of X-rays as a means of on-line composition monitoring during electron beam (E B) melting of alloys. The
design and construction of the collimation and protection systems used for the EDX are described in Part I. In Part II, a
mathematical simulation of the heat, mass, and momentum transfer was performed for comparison to the EDX and vapor deposition
results. The predicted flow patterns and evaporation rates are used to explain the differences between the two experimental
methods. For the EDX spectra measured, the X-rays generated were from the center of the hearth where fluid flow rising from
the bulk of the pool is sufficient to maintain the bulk composition despite the high evaporative flux from the surface. The
flow moves radially outward from the center of the pool, with the volatile species being depleted. The vapor deposition technique
measures the entire region, giving an average surface composition, and it therefore differs from the EDX results, which gave
a near bulk composition. This combined study using in-situ EDX measurements and numerical simulations both provided an insight into the phenomena controlling the evaporation in an
EB-heated system and demonstrated the viability of using EDX to measure the bulk composition during EB melting processes. 相似文献
125.
We have developed and implemented techniques that double the performance of dynamically-typed object-oriented languages. Our SELF implementation runs twice as fast as the fastest Smalltalk implementation, despite SELF's lack of classes and explicit variables.To compensate for the absence of classes, our system uses implementation-levelmaps to transparently group objects cloned from the same prototype, providing data type information and eliminating the apparent space overhead for prototype-based systems. To compensate for dynamic typing, user-defined control structures, and the lack of explicit variables, our system dynamically compilesmultiple versions of a source method, eachcustomized according to its receiver's map. Within each version the type of the receiver is fixed, and thus the compiler can statically bind andinline all messages sent toself.Message splitting andtype prediction extract and preserve even more static type information, allowing the compiler to inline many other messages. Inlining dramatically improves performance and eliminates the need to hard-wire low-level methods such as+, ==, andifTrue:.Despite inlining and other optimizations, our system still supports interactive programming environments. The system traverses internal dependency lists to invalidate all compiled methods affected by a programming change. The debugger reconstructs inlined stack frames from compiler-generated debugging information, making inlining invisible to the SELF programmer.This work has been generously supported by National Science Foundation Presidential Young Investigator Grant #CCR-8657631, and by IBM, Texas Instruments, NCR, Tandem Computers, Apple Computer, and Sun Microsystems.This paper was originally published inOOPSLA '89 Conference Proceedings (SIGPLAN Notices, 25, 10 (1989) 49–70). 相似文献
126.
Andre C.L. Carlin J.A. Boeckl J.J. Wilt D.M. Smith M.A. Pitera A.J. Lee M.L. Fitzgerald E.A. Ringel S.A. 《Electron Devices, IEEE Transactions on》2005,52(6):1055-1060
High-performance p/sup +//n GaAs solar cells were grown and processed on compositionally graded Ge-Si/sub 1-x/Ge/sub x/-Si (SiGe) substrates. Total area efficiencies of 18.1% under the AM1.5-G spectrum were measured for 0.0444 cm/sup 2/ solar cells. This high efficiency is attributed to the very high open-circuit voltages (980 mV (AM0) and 973 mV (AM1.5-G)) that were achieved by the reduction in threading dislocation density enabled by the SiGe buffers, and thus reduced carrier recombination losses. This is the highest independently confirmed efficiency and open-circuit voltage for a GaAs solar cell grown on a Si-based substrate to date. Larger area solar cells were also studied in order to examine the impact of device area on GaAs-on-SiGe solar cell performance; we found that an increase in device area from 0.36 to 4.0 cm/sup 2/ did not degrade the measured performance characteristics for cells processed on identical substrates. Moreover, the device performance uniformity for large area heteroepitaxial cells is consistent with that of homoepitaxial cells; thus, device growth and processing on SiGe substrates did not introduce added performance variations. These results demonstrate that using SiGe interlayers to produce "virtual" Ge substrates may provide a robust method for scaleable integration of high performance III-V photovoltaics devices with large area Si wafers. 相似文献
127.
Chia-Ching Lin Gwo-Yun Lee Kin-Lu Wong 《Electronics letters》2003,39(18):1302-1304
A surface-mount dual-loop antenna suitable for dual-frequency WLAN operation is presented. For achieving dual-frequency operation with a compact size, the antenna comprises two loop strips of different sizes printed on a flexible printed circuit board, which is then bent and attached onto a foam base of compact size. The antenna shows an attractive feature of high antenna gain, about 4 and 5 dBi for frequencies across the 2.4 and 5 GHz WLAN bands, respectively. 相似文献
128.
In this paper, a Ka-band thin film microstrip line(TFMS) filter with multi-layer structure on silicon substrate is introduced. The simulated results show that it is possible to design compact millimeter-wave filter on silicon substrate with process compatible to current VLSI technology. 相似文献
129.
Hyukmin Son Sanghoon Lee 《Communications Letters, IEEE》2006,10(5):360-362
A cell planning technique termed BRD (bandwidth and region division) is presented for overcoming interference, maintaining QoS (quality of service) and improving channel capacity over OFDM (orthogonal frequency division multiplexing)-based broadband cellular networks. Through an optimal combination of sectorization and zero padding, bandwidth and region division is achieved that minimizes the outage probability for forward-link cell planning. In order to verify the effectiveness of the proposed algorithm, a Monte Carlo simulation is performed over multi-cell environments. 相似文献
130.
We study a practical approach to match the performance of an output-queued switch statistically. For this purpose, we propose a novel switching architecture called a multiple input/output-queued (MIOQ) switch that requires no speedup for providing sufficient switching bandwidth. To operate an MIOQ switch in a practical manner, we also propose a multitoken-based arbiter which schedules the switch at a high operation rate and a virtual first-in first-out queueing scheme which guarantees the departure order of cells belonging to the same traffic flow at output. Additionally, we show that the proposed switch can naturally provide asymmetric bandwidth for inputs and outputs, which may be important in dealing with the links with different bandwidth demands. Finally, we compare the performance of an MIOQ switch with that of an output-queued switch and discuss the design criteria to match the performance of an output-queued switch. 相似文献