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61.
MJ Soltis HJ Yeh KA Cole N Whittaker RP Wersto EC Kohn 《Canadian Metallurgical Quarterly》1996,24(7):799-806
BACKGROUND: Bronchoscopic bronchoalveolar lavage in children to investigate bronchial disorders such as asthma has both ethical and procedural difficulties. OBJECTIVE: The aim of this study was to establish a standardized non-bronchoscopic method to perform bronchoalveolar lavage in children attending for elective surgery to obtain normal cellular data. METHODS: Bronchoalveolar lavage was performed on normal children (n = 55) by infusing saline (20 mL) through an 8 FG suction catheter passed after endotracheal intubation. Oxygen saturation, heart and respiratory rate were monitored during the bronchoalveolar lavage procedure. Cellular analysis and total protein estimation of the lavage fluid were performed. Epithelial lining fluid volume was calculated (n = 15) using the urea dilution method. RESULTS: The procedure was well tolerated by all children. Total cell count and differential cell count for children (macrophages 70.8 +/- 2.3%, lymphocytes 3.8 +/- 0.6%, neutrophils 5.7 +/- 1.0%, eosinophils 0.14 +/- 0.03%, epithelial cells 19.6 +/- 2.1%, mast cells 0.21 +/- 0.02%) were similar to those reported for adults. Age and sex comparisons revealed no differences between groups. The mean total protein recovered in the cell free supernatant was 49.72 +/- 4.29 mg/L and epithelial lining fluid volume was 0.82 +/- 0.11% of return lavageate. CONCLUSION: This method allows bronchoalveolar lavage to be performed safely and quickly on children attending for routine elective surgery. Using this method and taking the 'window of opportunity' of elective surgery, the presence or absence of airway inflammation could be studied in children with various patterns of asthma during relatively asymptomatic periods. 相似文献
62.
M Miampamba EJ Parr DM McCafferty JL Wallace KA Sharkey 《Canadian Metallurgical Quarterly》1998,12(3):219-228
BACKGROUND: We investigated the effects of benzalkonium chloride (BAC) on trinitrobenzene sulphonic acid (TNBS)-induced colitis in rats. METHODS: TNBS was administered intrarectally before and/or after BAC treatment. In the first study, the effects of treatment with BAC 6, 12 or 24 h after TNBS were examined. In the second study, animals were treated with BAC before, after or before and after TNBS, and were examined 7 days later. The severity of colitis was assessed by macroscopic and histological scoring of the colonic damage and by determination of colonic myeloperoxidase (MPO) activity. Macrophages and CD4+ and CD8+ T cells were examined by immunohistochemistry. RESULTS: When BAC was instilled into the colon 6, 12 or 24 h after TNBS, weight loss and macroscopic and histological features of the colon were similar to that of controls (TNBS alone). In contrast, MPO activity was significantly reduced in all three groups post-treated with BAC. In the groups examined 7 days after TNBS treatment, rats post-treated with BAC exhibited increased weight gain and significantly reduced macroscopic damage and MPO activity compared to the TNBS control group. Rats pre-treated with BAC exhibited less macroscopic damage of the colon than rats receiving only TNBS, but histological damage, MPO and weight gain were unchanged from TNBS controls. Immunohistochemistry revealed that BAC pre-treatment increased the numbers of macrophages and T cells in the colon. After TNBS treatment, macrophage accumulation was evident in the colon, but T cells were scarce. However, these cells were preserved or enhanced in the colonic mucosa in TNBS-treated rats that had been pre-treated with BAC. CONCLUSIONS: Treatment with BAC, particularly after induction of colitis, produces a significant reduction in the severity of tissue injury and inflammation through mechanisms that are not fully understood. 相似文献
63.
Three replica gratings were made from a single master; two were epoxy overcoated with different thicknesses of gold, and one was replicated directly in gold. Their performance in EUV light at grazing incidence angles was measured and compared. 相似文献
64.
Optimization of decision rules for hydroelectric operation to reduce both eel mortality and unnecessary turbine shutdown: A search for a win–win solution
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Worldwide populations of freshwater eels have declined with one of the contributing causes related to mortality during passage through hydropower turbines. An inherent trade‐off underlies turbine management where the competing demand for more hydropower comes at the expense of eel survival. A win–win solution exists when an option performs better on all competing demands compared to other options. A predictive model for eel migration based on a recent telemetry study was used to develop decision rules for turbine management in the Shenandoah River system. The performance of alternative decision rules was compared to the status quo policy to search for win–win solutions. Decision rules were defined by the probability of eel movement and were evaluated by the probabilities of false positive and false negative errors. The exact value of the cut‐off probability used in the decision rule will need to be determined through negotiation between stakeholders, but a range of cut‐off probabilities resulted in a win–win situation with both reduced eel mortality and increased turbine operation relative to the current shutdown strategy. Monitoring the implementation is needed to evaluate and update the predictive model and to refine the decision rule. Although the decision is framed for the Shenandoah River system, the analytical approach could be used to develop decision rules for turbine shutdown policy in other areas. 相似文献
65.
Unoccupied beaver (Castor canadensis) sites in New York State were for two years experimentally scented with a mixture of beaver castoreum and anal gland secretion. These sites were colonized less often than unscented control sites. The beaver is the first mammal to have been shown experimentally to use intraspecific odor cues when settling in vacant habitat. Territorial pheromones may be useful as repellents for beaver or other rodents. 相似文献
66.
One method for improving the quality of astronomical images measured through a atmospheric turbulence uses simultaneous short-exposure measurements of both an image and the output of a wave-front sensor exposed to an image of the telescope pupil. The wave-front sensor measurements are used to reconstruct an estimate of the instantaneous generalized pupil function of the telescope, which is used to compute an estimate of the instantaneous optical transfer function, which is then used in a deconvolution procedure. This imaging method has been called both deconvolution from wave-front sensor (DWFS) measurements and self-referenced speckle holography. We analyze the signal-to-noise ratio (SNR) behavior of this imaging method in the spatial frequency domain. The analysis includes effects arising from differences in the correlation properties of the incident and the estimated pupil phases and the fact that the object-spectrum estimator is a randomly filtered doubly stochastic Poisson random process. SNR resultsobtained for the DWFS method are compared with the speckle-imaging powerspectrum SNR for equivalent seeing conditions and light levels. It is shown that for unresolved stars the power-spectrum SNR is superior to the DWFS SNR. However, for extended objects the power-spectrum SNR and the DWFS SNR are similar. Since speckle imaging uses a separate Fourier phasereconstruction process not required by the DWFS method, the DWFS method provides an alternative to speckle imaging that uses simple postprocessing at the cost of a wave-front sensor measurement but with no loss of SNR performance for extended objects. 相似文献
67.
In the theory of atmospheric turbulence, the strength of the spatial variations of the index of refraction n is proportional to a parameter known as the atmospheric-structure constant. The atmosphericstructure constant is denoted C(2)(n)(z) and is a function of position along the optical path z. The characteristics of the temporal variations of the index of refraction are related to both C(2)(n)(z) and to the transverse wind velocity V(z). Current optical techniques for remotely sensing C(2)(n)(z) and V(z) rely primarily on the spatial or temporal cross-correlation properties of the intensity of the optical field. In the remote-sensing technique proposed here, we exploit the correlation properties of the wave-front slope measured from two point sources to obtain profiles of C(2)(n)(z) and V(z). The two sources are arranged to give crossed optical paths. The geometry of the crossed paths and the characteristics of the wave-front slope sensor determine the achievable resolution. The signal-to-noise ratio calculationsindicate the need for multiple measurements to obtain useful estimates of the desired quantities. 相似文献
68.
The S2 state electron paramagnetic resonance (EPR) multiline signal of Photosystem II has been simulated at Q-band (35 Ghz), X-band (9 GHz) and S-band (4 GHz) frequencies. The model used for the simulation assumes that the signal arises from an essentially magnetically isolated MnIII-MnIV dimer, with a ground state electronic spin ST = 1/2. The spectra are generated from exact numerical solution of a general spin Hamiltonian containing anisotropic hyperfine and quadrupolar interactions at both Mn nuclei. The features that distinguish the multiline from the EPR spectra of model manganese dimer complexes (additional width of the spectrum (195 mT), additional peaks (22), internal "superhyperfine" structure) are plausibly explained assuming an unusual ligand geometry at both Mn nuclei, giving rise to normally forbidden transitions from quadrupole interactions as well as hyperfine anisotropy. The fitted parameters indicate that the hyperfine and quadrupole interactions arise from Mn ions in low symmetry environments, corresponding approximately to the removal of one ligand from an octahedral geometry in both cases. For a quadrupole interaction of the magnitude indicated here to be present, the MnIII ion must be 5-coordinate and the MnIV 5-coordinate or possibly have a sixth, weakly bound ligand. The hyperfine parameters indicate a quasi-axial anisotropy at MnIII, which while consistent with Jahn-Teller distortion as expected for a d4 ion, corresponds here to the unpaired spin being in the ligand deficient, z direction of the molecular reference axis. The fitted parameters for MnIV are very unusual, showing a high degree of anisotropy not expected in a d3 ion. This degree of anisotropy could be qualitatively accounted for by a histidine ligand providing pi backbonding into the metal dxy orbital, together with a weakly bound or absent ligand in the x direction. 相似文献
69.
MD Dake DC Miller RS Mitchell CP Semba KA Moore T Sakai 《Canadian Metallurgical Quarterly》1998,116(5):689-703; discussion 703-4
OBJECTIVE: Our goal was to determine whether endovascular stent-grafting is feasible and effective for patients with aneurysms of the descending thoracic aorta. METHODS: Starting in July 1992, we conducted a prospective, uncontrolled clinical trial in 103 patients (mean age 69 years [range 34-89 years]) who underwent endovascular treatment of aneurysms of the descending thoracic aorta using a custom-fabricated, self-expanding stent-graft device. Follow-up was 100% complete and averaged 22 months. Sixty-two patients (60%) were judged not to be reasonable candidates for a conventional "open" surgical procedure. RESULTS: Complete thrombosis of the aneurysm was ultimately achieved in 86 (83%) patients. The early mortality rate was 9% +/- 3% (+/- 70% CL). Multivariable analysis revealed that myocardial infarction or stroke was linked with a higher likelihood of early death (P = .001). Early serious complications included paraplegia in 3% +/- 2% and stroke in 7% +/- 3%. Actuarial survival estimates at 1 year and 2 years were 81% +/- 4% and 73% +/- 5% (+/- 1 SE), respectively; being judged not to be a surgical candidate portended a higher probability of death (P = .003). According to the intent-to-treat principle, "treatment failure" (including all late sudden unexplained deaths) occurred in 38 patients; 53% +/- 10% of patients were free from treatment failure at 3.7 years. Stent-graft related complications occurred commonly and were linked with several anatomic, technical, and patient-related risk factors. CONCLUSIONS: This 5-year clinical trial involving use of a "first generation" device indicates that endovascular stent-grafting of descending thoracic aortic aneurysms is feasible with acceptable medium-term results. More refined, commercially developed devices available today offer less traumatic and more precise stent-graft deployment; these major technical advantages, coupled with important lessons we have learned over time and better patient selection, should be associated with more salutary clinical results in the future. 相似文献
70.
CF Chang LJ Kiser JE Bailey M Martins WC Gibson KA Schaberg DM Mirvis WB Applegate 《Canadian Metallurgical Quarterly》1998,279(11):864-869
In July 1996, Tennessee initiated a managed mental health and substance abuse program called TennCare Partners. This publicly funded "carve-out" experiment started chaotically and soon deteriorated into a crisis. Many patients did not receive care or lost continuity of care, and the traditional "safety net" mental health system nearly disintegrated. This qualitative case study sought to ascertain the impact of the TennCare Partners program. It points out that the program's difficulties stemmed directly from a flawed design that spread funds previously earmarked for severely mentally ill patients across the entire Medicaid population. States contemplating similar reforms should strive to protect vulnerable patients by risk-adjusting capitation payments and by focusing resources on care for severely mentally ill persons. States should also minimize program complexity and ensure the accountability of managed care networks for their patients' behavioral health care needs. 相似文献