Extensive restriction site polymorphism at the human phenylalanine hydroxylase locus and application in prenatal diagnosis of phenylketonuria

A total of 10 restriction site polymorphisms have been identified at the human phenylalanine hydroxylase locus using a full-length human phenylalanine hydroxylase cDNA clone as a hybridization probe to analyze human genomic DNA. These polymorphic patterns segregate in a Mendelian fashion and concordantly with the disease state in various PKU kindreds. The frequencies of the restriction site polymorphisms at the human phenylalanine hydroxylase locus among Caucasians are such that the observed heterozygosity in the population is 87.5%. Thus, most families with a history of classical phenylketonuria can take advantage of the genetic analysis for prenatal diagnosis and carrier detection of the hereditary disorder.     

Effect of nisin on the storage of sous vide processed Korean seasoned beef

Seasoned beef called Jangzorim in Korea is produced by boiling in soy sauce, and is a popular food in Korea. The aim of this study is to evaluate the microbial safety and physical qualities of sous vide processed seasoned beef, and the effect of nisin during storage. Sous vide processed packages with or without nisin (100 IU or 500 IU) were stored at 4 °C or 25 °C for 60 days, and samples measured for quality at regular intervals throughout this storage period. In the case of 25 °C storage, the number of mesophilic microorganisms in seasoned beef packages without nisin increased markedly, but with nisin there was no observed increase. Psychrotrophic microorganisms, anaerobic microorganisms, and B. cereus cells showed similar trends, although C. perfringens was not detected in all samples. At 25 °C storage, changes in the cutting force of packages containing nisin showed no significant change, packages without nisin decreased markedly. The colour of packages without nisin showed a drastic decrease in lightness (‘L’) while no changes were observed in packages with nisin.     

Shelf‐life characteristics of fresh oysters and ground beef as affected by bacteriocin‐coated plastic packaging film

Fresh oysters and ground beef were wrapped in antimicrobial films coated with a bacteriocin (nisin or lacticin NK24) incorporated into a polyamide binder layer. The packaged foods were stored at 3 and 10 °C and changes in counts of total aerobic bacteria and coliform bacteria were monitored together with appropriate quality attributes in order to determine the influence of the bacteriocin‐coated films on quality preservation and shelf‐life extension. Compared to plain low‐density polyethylene film, plastic films with incorporated bacteriocins slowed down microbial growth on packaged oysters and ground beef at both temperatures, contributed in some degree to the preservation of chemical quality and extended shelf‐life significantly. The effects of the antimicrobial films on the suppression of coliform bacterial growth were more pronounced at 10 than at 3 °C , while the effects on total aerobic bacteria were consistently evident at both temperatures. There was no difference in food quality preservation between the two types of antimicrobial film. © 2002 Society of Chemical Industry     

Post-Catch-Up Strategy for Medium-Sized South Korean Firms: Improving Technological Capabilities by Balancing R&D Intensity and Open Innovation

Abstract

This study examines how R&D intensity and open innovation affect the technological capabilities of firms in newly-developed countries, such as South Korea. East Asian latecomers are transitioning from a catch-up phase toward becoming leaders, and they are engaging in open innovation activities to drive this transition. This research quantitatively analyzes three kinds of open innovation activities—outside-in, inside-out, and coupled—to investigate their influence upon technological capabilities, while also considering the effect of R&D intensity. Data from 75 South Korean medium-sized firms were analyzed, and two major findings are reported. First, technological capabilities are enhanced by some open innovation activities and by R&D intensity. Second, R&D intensity negatively moderates the impact of open innovation activities on technological capabilities. Therefore, it may be concluded that firms with relatively low R&D intensity will gain the most benefit from increasing their open innovation activities.     

Antimicrobial activity of acid-hydrolyzed Citrus unshiu peel extract in milk

Citrus fruit (Citrus unshiu) peels were extracted with hot water and then acid-hydrolyzed using hydrochloric acid. Antimicrobial activities of acid-hydrolyzed Citrus unshiu peel extract were evaluated against pathogenic bacteria, including Bacillus cereus, Staphylococcus aureus, and Listeria monocytogenes. Antilisterial effect was also determined by adding extracts at 1, 2, and 4% to whole, low-fat, and skim milk. The cell numbers of B. cereus, Staph. aureus, and L. monocytogenes cultures treated with acid-hydrolyzed extract for 12 h at 35°C were reduced from about 8 log cfu/mL to <1 log cfu/mL. Bacillus cereus was more sensitive to acid-hydrolyzed Citrus unshiu peel extract than were the other bacteria. The addition of 4% acid-hydrolyzed Citrus unshiu extracts to all types of milk inhibited the growth of L. monocytogenes within 1 d of storage at 4°C. The results indicated that Citrus unshiu peel extracts, after acid hydrolysis, effectively inhibited the growth of pathogenic bacteria. These findings indicate that acid hydrolysis of Citrus unshiu peel facilitates its use as a natural antimicrobial agent for food products.     

Interfacial Structure of Ordered Domains in Barium Lanthanum Magnesium Niobate

Microstructural studies on the interfacial boundaries of 1:1 and 1:2 ordered domains in (Ba_0.9La_0.1)(Mg_0.37Nb_0.63)O₃ were conducted using high-resolution transmission electron microscopy and X-ray diffractometry. Both 1:2 and 1:1 ordered domains coexisted in a fully ordered single grain. Each ordered domain occupied its own region, and the interfaces were atomically sharp and coherent. The wavelength of the superlattice modulation was ∼0.47 nm in the 1:1 ordered domain and ∼0.71 nm in the 1:2 ordered domain. The transition from the 1:2 ordered region to the 1:1 ordered region was clearly shown at the interface. These observations well support the structural models that have been previously presented.     

焊接导致被焊铝板的初始挠曲特性

众所周知,与其他因素相比焊接引起的初始挠曲会极大地影响被焊板的极限强度,这意味着在计算板的极限强度以前判别板的初始挠曲性状是极其重要的。本文旨在研究海洋工程中应用的铝板在焊接过程中产生的初始挠曲特性。研究中共有78个单跨和多跨加劲平板铝结构,其与80m长全铝制高速船的基础结构同尺寸,采用金属惰性气体焊接。然后计算出加劲肋之间的初始挠曲。通过对数据库进行统计分析,得到初始挠曲变化系数。研究结果有助于焊接铝板结构的可靠性分析、极限强度确定和制作工艺控制。     

Separation of oxide inclusions from liquid metal in an applied electrostatic field

It is known that oxide inclusions in liquid metal carry mostly positive charges on their surfaces. In an electrostatic field, therefore, such charged particles suspended in a liquid metal experience forces and accumulate in the region of the negatively charged surface, resulting in the separation of oxide inclusions from the liquid metal. In this study, this principle was experimentally demonstrated for the case of a capacitor cell by the imposition of a d.c. potential on electrodes. The capacitor cell consisted of a dielectric container of Pyrex tube, a high voltage d.c. source, and two electrodes, which were symmetrically attached onto the outer surface of the Pyrex. tube. Experiments were carried out for suspensions of liquid tin/metal oxides, such as SnO₂, WO₃, and PbO under an applied potential of up to 12 kV. All experimental results were in good agreement with the theoretical prediction and showed that the degree of separation was significantly increased with the applied potential.     

Characterization of IS1547, a new member of the IS900 family in the Mycobacterium tuberculosis complex, and its association with IS6110

Unlike classically defined insertion sequence (IS) elements, which are delimited by their inverted terminal repeats, some IS elements do not have inverted terminal repeats. Among this group of atypical IS elements, IS116, IS900, IS901, and IS1110 have been proposed as members of the IS900 family of elements, not only because they do not have inverted terminal repeats but also because they share other features such as homologous transposases and particular insertion sites. In this study, we report a newly identified IS sequence, IS1547, which was first identified in a clinical isolate of Mycobacterium tuberculosis. Its structure, insertion site, and putative transposase all conform with the conventions of the IS900 family, suggesting that it is a new member of this family. IS1547 was detected only in isolates of the M. tuberculosis complex, where it had highly polymorphic restriction fragment length polymorphism patterns, suggesting that it may be a useful genetic marker for identifying isolates of the M. tuberculosis complex and for distinguishing different strains of M. tuberculosis. ipl is a preferential locus for IS6110 insertion where there are eight known different insertion sites for IS6110. Surprisingly, the DNA sequence of ipl is now known to be a part of IS1547, meaning that IS1547 is a preferential site for IS6110 insertion.