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91.
The volume of industrial lignin is expected to increase with the deployment of biorefineries that convert lignocellulosic biomass to renewable chemicals and fuels. Interest in using lignin for value-added biomedical applications requires understanding of its effects on mammalian and microbial cells, which has been impaired by the toxicity of the solvents used to solubilize lignin. In this study, lignin is solvated in zwitterionic Good's buffers compatible with culture media. Up to 100 mg lignin can be solvated in 1 ml of 3-morpholinopropane-1-sulfonic acid (MOPS, pH 7.2) within 60 min at room temperature, whereby MOPS acts as a chaotropic agent. The addition of MOPS-solvated lignin to cultures of Staphylococcus aureus UAMS-1 containing a subinhibitory concentration of tunicamycin reduced growth more than 99% compared to tunicamycin alone, making lignin of interest as an antibiotic adjuvant. This effect of lignin is attributed to damage to the bacterial cell membrane. 相似文献
92.
Hoelzer K Sauders BD Sanchez MD Olsen PT Pickett MM Mangione KJ Rice DH Corby J Stich S Fortes ED Roof SE Grohn YT Wiedmann M Oliver HF 《Journal of food protection》2011,74(7):1083-1095
Despite growing concerns about cross-contamination of ready-to-eat foods with Listeria monocytogenes, our knowledge about the ecology and transmission of L. monocytogenes in retail establishments has remained limited. We conducted a cross-sectional study to characterize the prevalence, distribution, and subtype diversity of L. monocytogenes in 120 New York State retail deli establishments that were hypothesized to present an increased risk for environmental L. monocytogenes contamination (i.e., small establishments and establishments with a history of failed New York State Agriculture and Markets inspections). Analysis of these data along with previously reported data for 121 predominantly larger retail establishments in New York State identified establishment size, geographic location, and inspection history as significant predictors of L. monocytogenes presence and prevalence. The odds of an establishment being L. monocytogenes positive were approximately twice as high for large establishments, establishments located in New York City, or establishments with poor inspection history (as compared with establishments without these attributes), even though correlation between location and inspection history complicated interpretation of results. Within an establishment, L. monocytogenes was significantly more prevalent on nonfood contact surfaces than on food contact surfaces; prevalence was particularly high for floors and in floor drains, sinks, the dairy case, and milk crates. L. monocytogenes subtype diversity differed between sites, with lineage I isolates significantly associated with nonfood contact surfaces and lineage II isolates significantly associated with food contact surfaces. Isolates belonging to the same ribotype were often found dispersed across multiple sites within an operation. 相似文献
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95.
Human capital and members of the creative class are bearers of economic growth, yet little is known about exactly what the relevant factors are for the concentration of the highly skilled in a specific place. Tolerance for example is supposed to make the difference between creative and human capital. But does tolerance really make a difference for anybody? And what about other factors: Are they specifically relevant for creative individuals or simply valid for the whole population? This study contributes to the discussion on the highly skilled by investigating whether tolerance, taxes, or other regional amenities contribute to their concentration and dynamics. The results show that tolerance in particular toward immigrants, but also toward same‐sex partnerships, is a rather dynamic concept, differs largely between and within functional urban regions, and makes a difference regarding the highly skilled. 相似文献
96.
JA Turpin Y Song JK Inman M Huang A Wallqvist A Maynard DG Covell WG Rice E Appella 《Canadian Metallurgical Quarterly》1999,42(1):67-86
Nucleocapsid p7 protein (NCp7) zinc finger domains of the human immunodeficiency virus type 1 (HIV-1) are being developed as antiviral targets due to their key roles in viral replication and their mutationally nonpermissive nature. On the basis of our experience with symmetrical disulfide benzamides (DIBAs; Rice et al. Science 1995, 270, 1194-1197), we synthesized and evaluated variants of these dimers, including sets of 4,4'- and 3,3'-disubstituted diphenyl sulfones and their monomeric benzisothiazolone derivatives (BITA). BITAs generally exhibited diminished antiviral potency when compared to their disulfide precursors. Novel, monomeric structures were created by linking haloalkanoyl groups to the benzamide ring through -NH-C(=O)- (amide) or -S-C(=O)- (thiolester) bridges. Amide-linked compounds generally lacked antiviral activity, while haloalkanoyl thiolesters and non-halogen-bearing analogues frequently exhibited acceptable antiviral potency, thus establishing thiolester benzamides per se as a new anti-HIV chemotype. Pyridinioalkanoyl thiolesters (PATEs) exhibited superior anti-HIV-1 activity with minimal cellular toxicity and appreciable water solubility. PATEs were shown to preferentially target the NCp7 Zn finger when tested against other molecular targets, thus identifying thiolester benzamides, and PATEs in particular, as novel NCp7 Zn finger inhibitors for in vivo studies. 相似文献
97.
Mass balances of total arsenic and copper for a suburban lake in densely populated northern Virginia were calculated using date collected during 1998. Mass-balance terms were precipitation; stream inflow, including road runoff; stream outflow; and contributions from leaching of pressure-treated lumber. More mass of arsenic and copper was input to the lake than was output the 1998 lake-retention rates were 70% for arsenic and 20% for copper. The arsenic mass balance compared well with a calculated annual mass accumulation in the top 1 cm of the lake sediments; however, the calculated contribution of copper to the lake was insufficient to account for the amount of copper in this zone. Leaching experiments were conducted on lumber treated with chromated copper arsenate (CCA) to quantify approximate amounts of arsenic and copper contributed by this source. Sources to lake sediments included leaching of CCA-treated lumber (arsenic, 50%; copper, 4%), streamwater (arsenic, 50%; copper, 90%), and atmospheric deposition (arsenic, 1%; copper, 3%). Results of this study suggest that CCA-treated lumber and road runoff could be significant nonpoint sources of arsenic and copper, respectively, in suburban catchments. 相似文献
98.
Di Bias A. Dahle D.M. Diekhans M. Grate L. Hirschberg J. Karplus K. Keller H. Kendrick M. Mesa-Martinez F.J. Pease D. Rice E. Schultz A. Speck D. Hughey R. 《Parallel and Distributed Systems, IEEE Transactions on》2005,16(1):80-92
The architectural landscape of high-performance computing stretches from superscalar uniprocessor to explicitly parallel systems, to dedicated hardware implementations of algorithms. Single-purpose hardware can achieve the highest performance and uniprocessors can be the most programmable. Between these extremes, programmable and reconfigurable architectures provide a wide range of choice in flexibility, programmability, computational density, and performance. The UCSC Kestrel parallel processor strives to attain single-purpose performance while maintaining user programmability. Kestrel is a single-instruction stream, multiple-data stream (SIMD) parallel processor with a 512-element linear array of 8-bit processing elements. The system design focuses on efficient high-throughput DNA and protein sequence analysis, but its programmability enables high performance on computational chemistry, image processing, machine learning, and other applications. The Kestrel system has had unexpected longevity in its utility due to a careful design and analysis process. Experience with the system leads to the conclusion that programmable SIMD architectures can excel in both programmability and performance. This work presents the architecture, implementation, applications, and observations of the Kestrel project at the University of California at Santa Cruz. 相似文献
99.
Garfinkel R. Gopal R.D. Nunez M. Rice D.O. 《IEEE transactions on systems, man, and cybernetics. Part A, Systems and humans : a publication of the IEEE Systems, Man, and Cybernetics Society》2006,36(3):461-471
Technological advances in the collection, storage, and analysis of data have increased the ease with which businesses can make profitable use of information about individuals. Some of this information is private, and individuals are simultaneously becoming more aware of the value of the information and how the loss of control over this information impacts their personal privacy. As a partial solution to these concerns, this paper presents a mechanism that serves two purposes. The first enables the use of private, numerical data in the answering of queries while simultaneously providing a security feature that protects the data owners from a loss of privacy that could result from an unauthorized access. The second develops a compensation model for the use of the data that allows individuals to dynamically redefine their security requirements. The compensation model is built on the information-security mechanism to create the foundation of a market for private information. This paper illustrates how compensation models like the one presented here could be used in a self-regulating market for private information. Additionally, the compensation component of an intermediated market for private information is developed and extensively analyzed. Finally, this paper provides insights and draws several important conclusions on markets for private information. 相似文献
100.
The work presented here demonstrates the first phases of a newly-proposed gun propellant formulation process that will minimize
life-cycle costs through science-based design. This new approach to gun propellant formulation proposes maximal use of modeling
and simulation in the earliest phases of the developmental cycle to screen candidate formulations, resulting in the elimination
of probable poor performers and the identification of the most promising candidates for further study and testing. The screening
and identification of promising candidate formulations is demonstrated in the study presented here under the assumption of
a specific weapon platform and user requirements. The process of selecting a propellant for the assumed gun system application
has been distilled into a series of measurable steps leading from a set of candidate materials, through logical and numerical
filters, to a shorter list of energetic materials demonstrated as viable choices for the weapon platform. Environmental filtering
and performance modeling are used to screen propellants through a well-defined sequence of tests designed to weed out materials
that do not meet standards in terms of safety, energy, or manufacturability. Because much of the testing is performed by computer
modeling, the gun systems and energetic materials need not be present (nor even existent) in order to be described and matched
against performance requirements for future applications. The calculations presented here demonstrate that utilizing computer
models in the early developmental stages of the formulation process rather than physical testing produces enormous savings
in labor, material, and environmental costs, along with a tremendous reduction in the time required to select a "best candidate"
propellant.
Received: 3 August 1999 / Accepted: 9 November 1999 相似文献