内热式液态合金离子源

本文介绍了一种实用的液态合金离子源结构，我们利用本结构研制成功了Ａｕ－Ｓｉ和Ａｕ－Ｓｉ－Ｂｅ液态合金离子源，测出了它们的发射特性。     

Adeno-associated virus 2-mediated high efficiency gene transfer into immature and mature subsets of hematopoietic progenitor cells in human umbilical cord blood

Recombinant adeno-associated virus 2 (AAV) virions were constructed containing a gene for resistance to neomycin (neoR), under the control of either the herpesvirus thymidine kinase (TK) gene promoter (vTK-Neo), or the human parvovirus B19 p6 promoter (vB19-Neo), as well as those containing an upstream erythroid cell-specific enhancer (HS-2) from the locus control region of the human beta-globin gene cluster (vHS2-TK-Neo; vHS2-B19-Neo). These recombinant virions were used to infect either low density or highly enriched populations of CD34+ cells isolated from human umbilical cord blood. In clonogenic assays initiated with cells infected with the different recombinant AAV-Neo virions, equivalent high frequency transduction of the neoR gene into slow-cycling multipotential, erythroid, and granulocyte/macrophage (GM) progenitor cells, including those with high proliferative potential, was obtained without prestimulation with growth factors, indicating that these immature and mature hematopoietic progenitor cells were susceptible to infection by the recombinant AAV virions. Successful transduction did not require and was not enhanced by prestimulation of these cell populations with cytokines. The functional activity of the transduced neo gene was evident by the development of resistance to the drug G418, a neomycin analogue. Individual high and low proliferative colony-forming unit (CFU)-GM, burst-forming unit-erythroid, and CFU-granulocyte erythroid macrophage megakaryocyte colonies from mock-infected, or the recombinant virus-infected cultures were subjected to polymerase chain reaction analysis using a neo-specific synthetic oligonucleotide primer pair. A 276-bp DNA fragment that hybridized with a neo-specific DNA probe on Southern blots was only detected in those colonies cloned from the recombinant virus-infected cells, indicating stable integration of the transduced neo gene. These studies suggest that parvovirus-based vectors may prove to be a useful alternative to the more commonly used retroviral vectors for high efficiency gene transfer into slow or noncycling primitive hematopoietic progenitor cells, without the need for growth factor stimulation, which could potentially lead to differentiation of these cells before transplantation.     

植被散射实验数据的特性分析

本文根据电波所获取的各种樾 射实测数据，分析了它们对不同极化波的响应特性。     

Reevaluation of Cl-/HCO3- exchange in cultured bovine corneal endothelial cells

PURPOSE: To determine the apical versus basolateral polarity of the putative anion exchanger in cultured bovine corneal endothelial cells (BCECs) and to examine the influence of Cl--dependent membrane potential (Em) changes on HCO3- transport. METHODS: BCECs grown on permeable supports were used for independent perfusion of apical and basolateral surfaces. Intracellular pH (pHi) was measured using the fluorescent dye BCECF. Relative changes in Em were measured using the fluorescent dye bis-oxonol. Western blot analysis was used to detect immunoreactivity against the anion exchanger (AE1 or AE2). RESULTS: Cl- removal from apical and basolateral surfaces produced cellular alkalinization (apical side, 0.07 pH units; basolateral side, 0.06 pH units; both sides, 0.20 pH units). Application of 100 microM H2-4,4'-diisothiocyanatodihydrostilbene-2,2'-disulfonic acid (DIDS), an anion exchange inhibitor, on the apical side produced an alkalinization (0.02 pH units) followed by acidification (-0.05 pH units), whereas basolateral H2DIDS caused a substantial acidification (-0.16 pH units). In the absence of Na+, Cl- removal from the apical side caused a transient alkalinization (0.03 pH units) followed by a return to baseline; Cl- removal from the basolateral side caused a small (-0.03) acidification. In Na+-free Ringer, apical H2DIDS produced a transient alkalinization (0.02 pH units), whereas basolateral exposure had no effect. 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), N-phenylanthranilic acid (DPC), and niflumic acid (50-200 microM), known Cl- channel blockers, produced cellular acidification in control Ringer. Niflumic acid hyperpolarized Em and inhibited depolarization after Cl- removal. Western blot analysis failed to detect AE2 expression in cultured BCECs. However, fresh BCECs produced a trace response. CONCLUSIONS: Physiological activity of an apical anion exchanger is weak in cultured BCECs. Cultured BCECs have significant Cl- conductance. Thus, cellular alkalinization after Cl- removal is caused primarily by depolarization of Em, which drives HCO3- influx through the basolateral electrogenic Na+:nHCO3- cotransporter. In contrast with cultured BCECs, AE2 may be present in fresh cells.     

Statistical analysis of decision threshold for parallel acquisitionwith reference filtering in Rician fading channel

Statistical analysis of the decision threshold for parallel PN code acquisition in a Rician fading channel is presented. It is shown that in the performance analysis of the acquisition system with reference filtering, the statistical evaluation of the decision threshold seems more appropriate than the approximation of the decision threshold     

钢铁表面锌系磷化膜的研究

本文采用ＳＥＭ和ＴＥＭ等测试手段，研究了四功能处理液不同条件下在钢铁表面上形成锌系磷化膜的形貌，晶体结构；采用ＥＤＳ研究了磷化膜的化学成发，通过实验确定了Ｚｎ／Ｐ值在２．１２～３．２０时，磷化膜具有良好的耐蚀性。     

Susceptibility of penicillin-susceptible and -resistant pneumococci to CFC-222, a new fluoroquinolone

Rotavirus (RV) strains infecting newborns often have unique neutralization antigens (P serotypes) on their outer capsids that are distinct from those found on RV strains that cause diarrhea in older children. We examined the hypothesis that unusual RV strains preferentially infect newborns because the newborns lack maternal neutralizing antibodies to these strains. To test this hypothesis, sera and saliva samples collected from neonates infected with 116E-like (P[11]G9) strains in the maternity ward of the All India Institute of Medical Sciences (AIIMS) hospital in New Delhi were tested for neutralizing antibodies against common RV strains and those infecting newborns and these titers were compared with those of newborns who did not become infected (controls). The infected neonates had significantly lower levels of cord blood neutralizing antibodies to 116E than the controls, suggesting that immunity to neonatal RV infection is acquired transplacentally through maternal antibodies. Further, this study confirmed the immunogenicity of the AIIMS neonatal strain 116E, a vaccine candidate, in its ability to evoke a potent RV-specific immunoglobulin A and neutralizing antibody response in serum and saliva among the infected babies. Our findings have important implications for the development of an effective RV vaccine. In India, where G9 strains are common in the community, the use of 116E as a vaccine, together with the rhesus tetravalent vaccine, may provide a broader protection against all the circulating RV serotypes, including serotype G9, which is not represented in the current rhesus RV tetravalent vaccine (G1-G4).     

Pre-bainitic Transformation in Fe-Ni Alloy

Ｔｈｅｐｒｅ－ｂａｉｎｉｔｉｃｐｈｅｎｏｍｅｎｏｎｉｓｏｎｅｏｆｔｈｅｉｍ－ｐｏｒｔａｎｔｓｕｂｊｅｃｔｓｏｆｂａｉｎｉｔｉｃｔｒａｎｓｆｏｒｍａｔｉｏｎｗｈｉｃｈｉｓｓｔｉｌｃｏｎｔｒｏｖｅｒｓｉａｌ．Ｏｎｅｖｉｅｗｉｓｔｈａｔｔｈｅｓｏｌｕｔｅａ...     

一种嵌入式软件的组合测试指标获取方案

为了获取组合测试中适应值函数所需要覆盖率、测试运行代价等信息,设计实现了基于嵌入式软件源代码插桩的指标获取方案。该方案使用交叉开发环境,完成了对源代码语法、词法分析,插桩库和插桩策略的制定,目标机／宿主机通讯方式设计,获得了语句覆盖率、分支覆盖率、运行时间、被测程序程序复杂度等关键指标,并以图表的方式提供统计结果,为组合测试设计适应值函数,进行测试用例约减提供了依据。     

自适应多采样扫描转换算法

随着片元着色器的可编程能力不断提高和新型图元的出现,扫描转换必须加强多采样时的处理能力.经典的边方程扫描转换算法便于实现多采样,但采样点测试的计算量大并且存在冗余测试,为此提出一种自适应多采样扫描转换算法.该算法的多采样集中在"边界片元",并结合点采样和区域采样技术根据自适应细分规则细分边界片元进行采样点的测试;避免了边方程算法中进行采样点测试时的乘法运算,减少了采样点的冗余测试,提高了多采样时扫描转换的性能.实验结果表明,文中算法在较小代价下达到或接近有相同采样点数的超采样反走样能力.