Dose-response feeding study of short chain chlorinated paraffins (SCCPs) in laying hens: effects on laying performance and tissue distribution, accumulation and elimination kinetics

Technical short chain chlorinated paraffins (C10-C13 with 60% chlorine) were fed to 93 laying hens from 24 to 32 weeks of age in increasing concentrations of up to 100 mg/kg feed. No significant influence on health, relative organ weights or performance (laying intensity, egg weight, feed consumption) was noted. The chlorinated paraffin content of the tissues was linearly related to the concentration of short chain paraffins of the feed. The highest concentrations were found in abdominal fat, egg yolk and fatty tissues. Breast muscle, egg albumen and bile fluid contained minimal or no residues. Less than 1% of the chlorinated paraffins ingested were incorporated into the body (without head, feet, gut and feathers), whereas about 1.5% were eliminated with the egg yolk and 30% were excreted with urine and faeces. A six-week kinetic depuration study revealed a biphasic elimination with half-lifes of 4-40 min (liver, kidneys, legs, fat, blood) for the initial rapid phase, and 15-30 days (blood, fat, liver, yolk, kidneys, legs) for the terminal slow phase.     

Determination of the absolute configurations of 4-mercapto-2-alkanones using the <Superscript>1</Superscript>H NMR anisotropy method and enzyme-catalyzed kinetic resolution of the corresponding 4-acetylthio-2-alkanones

To determine the capillary gas chromatographic (GC) elution order of the enantiomers of 4-mercapto-2-alkanones, racemic 4-acetylthio-2-alkanones were synthesized and hydrolyzed by lipases to get enantio-enriched 4-mercapto-2-alkanones that were resolved by capillary gas chromatography using a chiral stationary phase. The obtained enantio-enriched 4-mercapto-2-alkanones were esterified with (S)-2-methoxy-2-(1-naphthyl)propionic acid ((S)-MαNP acid), and the HPLC analysis of MαNP thioesters revealed the same isomeric ratios as the chiral GC analysis of the corresponding thiol enantiomers. The diastereoisomeric thioesters obtained were resolved by HPLC, and the absolute configurations were determined on the basis of ¹H NMR anisotropy effects. On the basis of these results, the GC elution order of the enantiomers of 4-mercapto-2-alkanones and of the corresponding 4-acetylthio-2-alkanones could be determined. The combination of enzymatic resolution and MαNP thioesterification proved to be a useful tool to determine the absolute configuration of secondary thiols.     

Solving constrained combinatorial optimization problems via importance sampling in the grand canonical ensemble

Combinatorial optimization problems are usually NP-hard. These problems are generally tackled by heuristic or branch-and-bound methods. The aim of this paper is to tackle constrained combinatorial optimization problems by importance Monte Carlo sampling. For this, we show that every constrained combinatorial optimization problem can be represented by a thermodynamical system in a suitable grand canonical ensemble given by the quantities of temperature, volume, and chemical potential. In order to find optimum solutions of the optimization problem, the grand canonical Monte Carlo method can be applied to the corresponding thermodynamical system. This method will amount to importance sampling, i.e. good feasible solutions of the optimization problem will be preferably sampled, provided that the intensive quantities of temperature and chemical potential are appropriately chosen. Our approach extends the standard importance sampling approach in the canonical ensemble to tackle unconstrained combinatorial optimization problems. The knapsack problem is considered as a prototype example.     

Die Bestimmung von Schwefelanteilen im Stahl unter 10 μg/g

Analytische Möglichkeiten einer neuen Gerätegeneration. Weiterentwicklung des Verfahrens mit synthetischer Eichung bis zur Bestimmungsgrenze 2 μg/g.     

Festlegung der physikalischen Wirkzusammenhänge,Unterprogramm PHYWIZ,beim rechnerunterstützten systematischen Projektieren und Konstruieren

Aufgabe des Unterprogrammes PHYWIZ. Ermittlung physikalischer Funktionen zur Erfüllung der Funktionen der festgelegten logischen Funktionskette. Variante physikalische Funktionen und Mehrfachzuordnungen. Programmstruktur zur Berechnung physikalischer Wirkzusammenhänge. Behandlung der unmittelbar schnittstellenabhängigen Zusammenhänge. Vorgehen bei Überschreitung der Schnittstellenbedingungen. Bearbeitung der physikalischen Wirkzusammenhänge, die keinen Einfluß auf die Schnittstellenbedingungen haben können. Bewertung varianter Funktionsketten und endgültige Festlegung auf eine physikalische Funktionskette.