Application of gas-liquid partition chromatography to the quantitative estimation of monoglycerides

Summary A procedure has been developed for the quantitative estimation of monoglycerides in terms of their constituent fatty acids. The monoglyceride mixture is mesylated with mesyl chloride in the presence of pyridine, and the resulting dimesyl derivatives are converted to allyl esters of the constituent fatty acids by treatment with sodium iodide in anhydrous acetone at 100°C. The allyl esters are then analyzed quantitatively by gas-liquid partition chromatography at 240°C. on a column of Apiezon M—Celite. Both α- and β-monoglycerides are quantitatively converted to allyl esters by this procedure. β-Monoglycerides in a mixture of α- and β-isomers may be determined separately after removal of the α-isomers by oxidation with periodic acid. The analytical procedure is also applicable to monoglycerides in the presence of free fatty acids, diglycerides, and triglycerides. Issued as N.R.C. No. 5438. Presented at the 32nd fall meeting, American Oil Chemists' Society, Chicago, Ill., October 20–22, 1958. National Research Council Postdoctorate Fellow, 1956–1958.     

Conceptual behavior in psychotic and normal adolescents.

This study has investigated conceptual behavior in 16 psychotic adolescents matched with 16 adjusted adolescents on age, IQ, and socioeconomic status. A picture sorting test was administered to both groups. Psychotic adolescents categorize and verbalize less adequately than matched controls. Psychotic adolescents use narrower categories than control Ss, perhaps reflecting an inability to reduce effectively the complexity of a stimulus array. In the qualitative analyses of verbalizations, it was found that psychotics use more idiosyncratic verbalizations, possibly indicating the greater privacy and autism of their thinking processes. The conceptual level of the verbalizations offered by the psychotics was less "abstract." Social and emotional content seems to have no effect on categorization and verbalization variables in psychotics. (16 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Perceptual categorizations of love and anger cues in schizophrenics.

The 1st study identified 4 filmed scenes indicative of high love, high anger, medium love, and medium anger. The 2nd study compared 30 good premorbid schizophrenics and 30 normals on the high love and high anger scenes. They were not differentiated on the high love scene. On the high anger scene, the normals reported significantly more anger than the good premorbids. The 3rd study evaluated 48 poor premorbid schizophrenics, 48 good premorbid schizophrenics, and 48 normals on 4 scenes of high love, high anger, medium love, and medium anger. The good premorbids and the normals were not different on the high love scene, but significantly different from the poor premorbids. On the high anger scene, good and poor premorbids were significantly different from normals. (18 ref.) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Patterns of growth deficiency in rats exposed in utero to undernutrition, ethanol, or the neuroteratogen methylazoxymethanol (MAM)

Children and experimental animals exposed to ethanol (EtOH) in utero commonly have low birthweights, and many remain small at maturity. Low body weight or small stature in adulthood may reflect an inability to recover from in utero growth retardation, or it may reflect a separate, postnatal growth deficiency. In this study, daily body weights (postnatal days 1 to 60) were compared among the offspring of the following groups of Long Evans rats: dams fed liquid diet containing 35% EtOH-derived calories; their pair-fed and chow-fed controls; and dams exposed to methylazoxymethanol (MAM) in two previous studies, in which offspring exhibited reduced numbers of growth hormone releasing factor (GRF) neurons. All treatments produced a number of offspring with weight deficits beginning after birth and persisting into maturity. Three distinct patterns of growth deficiency were observed: (1) weight loss relative to controls in the first weeks of life, seen in offspring exposed to EtOH, pair feeding, or MAM on gestation day 13 (G13); (2) a delay in the onset of the prepubertal growth spurt, seen in all EtOH-exposed offspring and in G13 MAM-exposed dwarfs; and (3) failure to sustain the prepubertal growth spurt, seen only after exposure to MAM on G14. The results of this study support the view that prenatal EtOH exposure is capable of affecting postnatal growth specifically; moreover, the pattern of growth deficiency seen in EtOH-exposed offspring was distinct from that of the undernourished offspring of pair-fed dams.     

Authoritarian ideology and attitudes on parent-child relationships.

"The relationship between authoritarian ideology and attitudes about parent-child relationships was investigated by testing 172 native-white, Christian university students." Findings indicate a relationship in females that could be related to personality dispositions. It was proposed that strong authoritarian beliefs may be maladjustive in a democratic society since such beliefs are associated with attitudes similar to those held by parents of problem children. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A comparison of attenuated total reflectance-FTIR spectroscopy and GPC for monitoring biodiesel production

Gel permeation chromatography (GPC) and attenuated total reflectance (ATR)-FTIR spectroscopy were used to monitor the products of transesterification of waste frying oil in methanol to FAME or biodiesel. To evaluate the reliability and reproducibility of each method, quantitative analyses of mixtures of standards (TG, DG, MG, FAME, and glycerol) as well as lipid products of transesterification were carried out. The reproducibility of each method was found to be within ±1–5%. The differences between the results of the two methods were less than ±2%. The GPC method showed good separation of the intermediate products MG and glycerol from the TG starting material and FAME, but DG were not completely separated from TG, GPC gave good quantitative results for MG and FAME, but the TG and DG analyses required correction, depending on the mole ratio of TG/DG. In contrast, ATR-FTIR spectroscopy could only give quantitative data for the sum of TG+DG+MG.