Potent bactericidal activity of bovine lactoferrin hydrolysate produced by heat treatment at acidic pH.

A hydrolysate of bovine lactoferrin produced by heat treatment under acidic conditions had antibacterial activity at concentrations of 10 micrograms/ml in the culture medium. The optimal degree of hydrolysis for this activity was about 10%. Heat-treated lactoferrin, treated at pH 2.0 and 120 degrees C for 15 min and degree of hydrolysis of about 10%, had no Fe-binding capacity (0%) and less antigenicity (about 10(-6) than untreated lactoferrin. Heat-treated lactoferrin increased in antibacterial activity, and the activity was maintained in an Fe-rich medium. After fractionation of heat-treated lactoferrin by reverse-phase HPLC, several peptide fractions were found that had strong antibacterial activity. It was suggested that lactoferrin latently contains at least one bactericidal domain that is activated upon release by limited acid hydrolysis of the protein. The bactericidal activity of the peptide fragments of lactoferrin was shown to have no relation to Fe chelation, in contrast with the antibacterial mechanism of native lactoferrin.     

Biomineral nanoparticles are space-filling

Sea urchin biominerals have been shown to form from aggregating nanoparticles of amorphous calcium carbonate (ACC), which then crystallize into macroscopic single crystals of calcite. Here we measure the surface areas of these biominerals and find them to be comparable to those of space-filling macroscopic geologic calcite crystals. These biominerals differ from synthetic mesocrystals, which are invariably porous. We propose that space-filling ACC is the structural precursor for echinoderm biominerals.     

Dynamic equilibrium of MoSi2 polymorphs during mechanical milling

The phase transformation of α-MoSi₂ into β-MoSi₂ induced by mechanical milling (MM) was studied. Planetary ball milling was performed on an α-MoSi₂ powder under six different milling conditions. The X-ray diffraction results show that the dynamic equilibrium between α-MoSi₂ and β-MoSi₂ is reached at low milling intensities, while a single phase of β-MoSi₂ is formed when milled at high milling intensities. The single-phase β-MoSi₂ formation is found to be due to Fe impurity. The mechanism of the phase transformation of MoSi₂ is discussed, and the phase fractions under the dynamic equilibrium are explained by the redistribution of the close-packed layers via synchroshear processes.     

Electrolyzer switching strategy for hydrogen generation from variable speed wind generator

This paper presents a new switching strategy for electrolyzer used in hydrogen generation which is connected to the terminal of a wind farm. The output of wind generator, in general, fluctuates greatly due to the random wind speed variations, which has a serious influence on the power system operation. In this study, the wind farm is composed of variable speed wind turbines (VSWT) driving permanent magnet synchronous generators (PMSG). The hydrogen generator (HG) is composed of rectifier and 10 electrolyzer units where each unit is controlled by the chopper circuit. To smoothen the wind farm line power, at first, a reference for the line power is generated from the difference of exponential moving average of wind farm output and its standard deviation. Then the switching strategy is developed in such a way that the proposed cooperative system can smoothen the wind farm line power fluctuation as well as generating hydrogen gas absorbing the fluctuating portion of wind farm output that lies above the reference line power. This novel switching strategy helps each electrolyzer unit working in full load and shift operation conditions and hence increases its lifespan and efficiency. The performance of the proposed system is investigated by simulation analyses, in which simulations are performed by using PSCAD/EMTDC.     

Effects of Synchronous Machine Saturation,Circuit Parameters,and Control Systems on Steady State Stability

Analysis of elementary power system configuration by means of idealized model illustrates some of the fundamental stability properties of power system. Instability in a power system may be manifested in many different ways, depending on the system configuration and operating mode. Analysis of synchronous machine performance, including saturation effect, is very important. Synchronous machine performance is greatly influenced by its iron saturation. The developed synchronous machine model, and hence the power system model, which takes this saturation effect into account will also depict the effects of the control systems on power system steady state stability. The objective of this paper is to provide an overview of the power system stability phenomena, which is also dependent on the synchronous machine circuit parameters with and without automatic voltage regulator and governor systems.     

Identification of genes whose expressions are enhanced or reduced in baker's yeast during fed-batch culture process using molasses medium by DNA microarray analysis

Genes whose expression levels are enhanced or reduced during the cultivation process that uses cane molasses in baker's yeast production were identified in this study. The results showed that baker's yeast grown in molasses medium had higher fermentation ability and stress tolerance compared with baker's yeast grown in synthetic medium. Molasses apparently provided not only sugar as a carbon source but also provided functional components that enhanced or reduced expression of genes involved in fermentation ability and stress tolerance. To identify the genes whose expression is enhanced or reduced during cultivation in molasses medium, DNA microarray analysis was then used to compare the gene expression profile of cells grown in molasses with that of cells grown in synthetic medium. To simulate the commercial baker's yeast production process, cells were cultivated using a fed-batch culture system. In molasses medium, genes involved in the synthesis or uptake of vitamins (e.g., biotin, pyridoxine and thiamine) showed enhanced expression, suggesting that vitamin concentrations in molasses medium were lower than those in synthetic medium. Genes involved in formate dehydrogenase and maltose assimilation showed enhanced expression in molasses medium. In contrast, genes involved in iron utilization (e.g., siderophore, iron transporter and ferroxidase) showed enhanced expression in synthetic medium, suggesting that iron starvation occurred. The genes involved in the metabolism of amino acids also showed enhanced expression in synthetic medium. This identification of genes provides information that will help improve the baker's yeast production process.     

GaInP single junction and GaInP/GaAs two junction thin-film solar cell structures by epitaxial lift-off

The epitaxial lift-off (ELO) technique was used in forming a thin-film GaInP/GaAs two-junction monolithic tandem solar cell structure. First, the GaInP single junction solar cell to be used in the tandem cell structure as a top cell was thinned by the ELO process. Although the ELO process and the transfer to the quartz substrate caused a strain in the thin-film cell after separation from the GaAs substrate, the photoluminescence peak intensity was not decreased. This shows that defects, such as those causing carrier loss, were not introduced on the thin-film cell during the thinning process. The key issue for thin-film cell fabrication is to avoid damaging the AlInP window layer during the selective etching (HF etchant), by which the thin-film cell is released from the GaAs substrate. A GaInP/GaAs monolithic tandem structure was also thinned by the same process with a GaInP single junction cell. Characteristics of the single-junction GaInP cell and individual cells in the GaInP/GaAs tandem structure were examined. It was found that the spectral response remains almost the same as that for cells with a GaAs substrate, thus confirming the feasibility of using the ELO process to fabricate thin-film GaInP/GaAs cells.     

Analysis of changing hidden energy flow in Vietnam

The energy consumption in production process is changing especially in developing countries by substituting technology. Input–output analysis for energy flows has been developing and is one of the best solutions for investigating macroscopic exchanges of both economy and energy. Since each element in the Leontief inverse contains both direct and indirect effects of any change in final demand, to separate those direct and indirect effects, the power series expansion is available. In this work, the changes of embodied energy intensity in Vietnam from 1996 to 2000 were analyzed using the structural decomposition and its power series expansion. By illustrating the change of causal relationship between direct energy consumption and embodied energy consumption, the change of hidden energy flow, which indicates how the changing embodied energy builds up the change of direct energy consumption in every sector, can be seen. In the case study, the rice processing sector, which is one of the important food processing sectors in Vietnam, is focused. By drawing a diagrammatic map for the change of hidden energy flow, it is clarified that in the case of raising embodied energy intensity, cultivation sector and trade and repaired service sector are the main contributors, and, on the contrary, in the case of reducing embodied energy intensity, paper pulp sector is the main contributor.