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91.
Cooper KM Tuomola M Lahdenperä S Lövgren T Elliott CT Kennedy DG 《Food Additives & Contaminants》2002,19(12):1130-1137
Zeranol, an oestrogenic growth promoter in food animals, is banned within the European Union (EU). However, commercially available immunoassay kits for zeranol cross-react with toxins formed by naturally occurring Fusarium spp. fungi, leading to false-positive screening results. This paper describes the validation of a specificity enhanced, rapid dry reagent time-resolved fluoroimmunoassay (TR-FIA) for zeranol (recovery 99%, limit of detection 1.3 ng ml(-1)) demonstrating that up to 150 ng ml(-1) of Fusarium spp. toxins in urine do not lead to false-positive results. This assay will assist EU Member States to implement Council Directive 96/23/EC, which requires states to monitor for potential abuses of zeranol. A similar TR-FIA for the Fusarium spp. toxin alpha-zearalenol, using the same sample extract, is also described (recovery 68%, limit of detection 5.6 ng ml(-1)). Only the addition of diluted sample extract is required to perform these dry-reagent TRFIAs, the results being available within 1h of extract application. The EU-funded project 'Natural Zeranol' (FAIR5-CT97-3443) will use these fluoroimmunoassays to screen bovine urine in four Member States to gather data on the seasonality of Fusarium spp. toxin contamination of urine and the incidence of zeranol screening test positives. 相似文献
92.
Kennedy CJ 《Applied optics》2002,41(21):4341-4346
A functional relationship between M2 of a multimode laser and its extraction efficiency from a uniformly pumped cylindrical laser medium is derived from numerical calculations by use of helicoid modes. 相似文献
93.
In many cases, simple analytical models used by traditional compilers are no longer able to yield effectively optimized code
for complex programs because of the enormous complexity of processor architectures. A promising alternative approach for optimizing
applications effectively has been the use of search-based empirical methods. The success of empirically tuned library generators
such as ATLAS has shown that this strategy can be effective for domain-specific programs. However, to date there has been
no general-purpose tool for effective empirical optimization of whole programs. The main obstacle to this approach has been
the need for evaluating a prohibitively large number of alternative program variants. To address this problem, we have developed
a prototype tool for automatic application tuning that uses loop-level performance feedback and a direct search strategy to
guide search for the best set of optimization parameters. Experiments on four different architectures show that direct search
can be an effective technique for finding good values for transformation parameters in a reasonable time.
This material is based on work supported by the Department of Energy under Contract Nos. 03891-001-99-4G, 74837-001-03 49,
86192-001-04 49, and/or 12783-001-05 49 from the Los Alamos National Laboratory. 相似文献
94.
Toward a systems- and control-oriented agent framework. 总被引:1,自引:0,他引:1
Kingsley Fregene Diane C Kennedy David W L Wang 《IEEE transactions on systems, man, and cybernetics. Part B, Cybernetics》2005,35(5):999-1012
This paper develops a systems- and control-oriented intelligent agent framework called the hybrid intelligent control agent (HICA), as well as its composition into specific kinds of multiagent systems. HICA is essentially developed around a hybrid control system core so that knowledge-based planning and coordination can be integrated with verified hybrid control primitives to achieve the coordinated control of multiple multimode dynamical systems. The scheme is applied to the control of teams of unmanned air and ground vehicles engaged in a pursuit-evasion war game. Results are demonstrated in simulation. 相似文献
95.
We present two methods for determining the sentiment expressed by a movie review. The semantic orientation of a review can be positive, negative, or neutral. We examine the effect of valence shifters on classifying the reviews. We examine three types of valence shifters: negations, intensifiers, and diminishers. Negations are used to reverse the semantic polarity of a particular term, while intensifiers and diminishers are used to increase and decrease, respectively, the degree to which a term is positive or negative. The first method classifies reviews based on the number of positive and negative terms they contain. We use the General Inquirer to identify positive and negative terms, as well as negation terms, intensifiers, and diminishers. We also use positive and negative terms from other sources, including a dictionary of synonym differences and a very large Web corpus. To compute corpus-based semantic orientation values of terms, we use their association scores with a small group of positive and negative terms. We show that extending the term-counting method with contextual valence shifters improves the accuracy of the classification. The second method uses a Machine Learning algorithm, Support Vector Machines. We start with unigram features and then add bigrams that consist of a valence shifter and another word. The accuracy of classification is very high, and the valence shifter bigrams slightly improve it. The features that contribute to the high accuracy are the words in the lists of positive and negative terms. Previous work focused on either the term-counting method or the Machine Learning method. We show that combining the two methods achieves better results than either method alone. 相似文献
96.
Model-updated image guidance: initial clinical experiences with gravity-induced brain deformation 总被引:1,自引:0,他引:1
Miga MI Paulsen KD Lemery JM Eisner SD Hartov A Kennedy FE Roberts DW 《IEEE transactions on medical imaging》1999,18(10):866-874
Image-guided neurosurgery relies on accurate registration of the patient, the preoperative image series, and the surgical instruments in the same coordinate space. Recent clinical reports have documented the magnitude of gravity-induced brain deformation in the operating room and suggest these levels of tissue motion may compromise the integrity of such systems. We are investigating a model-based strategy which exploits the wealth of readily-available preoperative information in conjunction with intraoperatively acquired data to construct and drive a three dimensional (3-D) computational model which estimates volumetric displacements in order to update the neuronavigational image set. Using model calculations, the preoperative image database can be deformed to generate a more accurate representation of the surgical focus during an operation. In this paper, we present a preliminary study of four patients that experienced substantial brain deformation from gravity and correlate cortical shift measurements with model predictions. Additionally, we illustrate our image deforming algorithm and demonstrate that preoperative image resolution is maintained. Results over the four cases show that the brain shifted, on average, 5.7 mm in the direction of gravity and that model predictions could reduce this misregistration error to an average of 1.2 mm. 相似文献
97.
L. E. Murr E. Ferreyra J. G. Maldonado E. A. Trillo S. Pappu C. Kennedy J. De Alba M. Posada D. P. Russell J. L. White 《Journal of Materials Science》1999,34(5):967-979
Steel-drum fabrication, especially the sinking of the drum head (also referred to as the pan) by hand with a hammer, has been examined in detail utilizing light metallography (LM) and transmission electron microscopy (TEM) to characterize residual microstructures corresponding to reductions in thickness of up to 50 % at the bottom of the drum head. Dislocation densities in the low-carbon (0.01–0.05 wt % C), ferritic steels can exceed 1010 cm-2. Simulations of simple, ideal, free circular notes utilizing 316 stainless-steel plates (0.05 wt % C), cold rolled to reductions up to 40%, revealed that deformation (per cent cold reduction) has an important effect on the acoustic spectrum, especially harmonic spectra. Harmonic-node splitting was observed for thin circular plates (0.076 cm thick); the frequency difference was 60 Hz at 20% cold reduction and 160 Hz at 40% cold reduction. These dispersion effects, due to deformation-induced microstructures, as well as irregularities in the note geometries and thicknesses, point to the complex and non-linear acoustic features that contribute to the unique sounds of the Caribbean steel drum. 相似文献
98.
OBJECTIVE: To assess the extent and content of newspaper and magazine coverage of research on passive smoking. DESIGN: Content analysis of newspaper and magazine articles. SUBJECTS: Articles reporting on passive smoking research published in newspapers (n = 180) or magazines (n = 92) between January 1981 and December 1994. MAIN OUTCOME MEASURES: Numbers of articles, conclusions of articles, sources quoted, numbers and characteristics of research studies cited, presence of tobacco advertising. RESULTS: The number of newspaper and magazine articles reporting on passive smoking research increased from four in 1981 to 57 in 1992 and 32 in 1994. Sixty-two per cent (168/272) of articles concluded that the research on passive smoking is controversial. Tobacco industry representatives were quoted significantly more often in newspaper articles (52%, 94/180) than magazine articles (12%, 11/92) (p<0.0001). Of 121 different research studies cited in the lay press articles, only 15 were from tobacco-industry sponsored projects or publications. In magazines, acceptance of tobacco industry advertising was associated with the conclusion that research on passive smoking is controversial (p<0.0001). CONCLUSIONS: Although research on the harmful effects of passive smoking accumulated between 1981 and 1994, lay press coverage of the research maintained that the science was controversial. Few research studies were cited to support the industry's claim that passive smoking is not harmful to health. However, tobacco industry representatives who were critical of the research methods used to study the health effects of passive smoking were frequently quoted. 相似文献
99.
Two methods for amperometric detection of exocytosis at single pancreatic beta-cells were compared. In the first, direct detection of insulin was accomplished using an insulin-sensitive chemically modified electrode. In the second, 5-hydroxytryptamine (5-HT) that had been allowed to accumulate within the beta-cell secretory vesicles was detected with a bare carbon electrode. The goal of the comparison was to determine whether 5-HT secretion was a valid marker of insulin secretion in single beta-cells. To aid in this comparison, some experiments involved simultaneous measurement of insulin and 5-HT at cells previously allowed to accumulate 5-HT. Upon application of common insulin secretagogues, current spikes resulting from detection of 5-HT, insulin, or both compounds were obtained indicative of secretion via exocytosis. The mean area of current spikes obtained from simultaneous measurements equaled the sum of the mean area of insulin and 5-HT measured independently. Additionally, analyses of the number of spikes obtained for detection of insulin, 5-HT, or both compounds were similar for several common secretagogues. These data support the hypothesis that accumulated 5-HT is released from insulin containing secretory vesicles, exclusively. In addition, measurement of insulin and 5-HT from beta-cells of different species was compared to determine whether a species dependence exists for the two methods compared here. Detection of 5-HT results in a similar number of spikes that are equivalent to insulin in frequency and amplitude in human, porcine, and canine beta-cells; however, in mouse and INS-1 beta-cells, 5-HT is more readily detected than insulin. 相似文献
100.