Evidence for Contributions of Interactions of Constituents to the Anti-Inflammatory Activity of Hypericum Perforatum

Hypericum perforatum (Hp) extracts contain many different classes of constituents including flavonoids and biflavonoids, phloroglucinols, naphthodianthrones, caffeic acid derivatives, and unknown and/or unidentified compounds. Many constituents may be responsible for the anti-inflammatory activity of Hp including quercetin and derivatives, hyperforin, pseudohypericin, and amentoflavone. In line with antidepressant data, it appears that the interactions of constituents may be important for the anti-inflammatory activity of Hp. Interactions of constituents, tested in bioavailability models, may explain why synergistic mechanisms have been found to be important for antidepressant and antiproliferative bioactivities. This review highlights the relationship among individual constituents and the anti-inflammatory activity of Hp extracts and proposes that interactions of constituents may be important for the anti-inflammatory activity of botanical extracts, although the exact mechanisms of the interactions are still unclear.     

Racial distinctions in the psychosocial histories of incarcerated youth.

This study examined the psychosocial histories of 134 male, juvenile offenders, focusing on race and the probability of being diagnosed with internalizing and/or externalizing psychological disorders at the time of their sentencing to a correctional institution for committing serious and violent crimes as minors. In addition, based on case file information, the author investigated offenders' histories with child maltreatment, court-ordered probation, and counseling services received as part of the child welfare and/or juvenile justice system. Results from logistic regression analyses indicated the probability for White youth to be diagnosed with internalizing disorders was over 19 times that of Black youth; however, rates of externalizing disorders were similar across groups. Although maltreatment histories were comparable based on race, White youth received more counseling services, were significantly older at the time of diagnosis, and were more likely to be diagnosed with internalizing and comorbid disorders. Issues regarding multicultural assessment in the child welfare and juvenile justice system are discussed, specifically highlighting the potential for attributional bias in the diagnostic and treatment recommendation process. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Strong enhancement of solar cell efficiency due to quantum dots with built-in charge

We report a 50% increase in the power conversion efficiency of InAs/GaAs quantum dot solar cells due to n-doping of the interdot space. The n-doped device was compared with GaAs reference cell, undoped, and p-doped devices. We found that the quantum dots with built-in charge (Q-BIC) enhance electron intersubband quantum dot transitions, suppress fast electron capture processes, and preclude deterioration of the open circuit voltage in the n-doped structures. These factors lead to enhanced harvesting and efficient conversion of IR energy in the Q-BIC solar cells.     

The Class D beta-lactamase family: residues governing the maintenance and diversity of function

Class D β-lactamases, a major source of bacterial resistance to β-lactam antibiotic therapies, represent a distinct subset of the β-lactamase superfamily. They share a serine hydrolase mechanism with Classes A/C vs. Class B. Further understanding of their sequence-structure-function relationships would benefit efforts to design a new generation of antibiotics as well as to predict evolutionary mechanisms in response to such therapies. Here we describe analyses based on our high-resolution multiple sequence alignment and phylogenetic tree of ～80 Class D β-lactamases that leverage several 3D structures of these enzymes. We observe several sequence clusters on the phylogenetic tree, some that are species specific while others include several species from α-, β- and γ-proteobacteria. Residues characteristic of a specific cluster were identified and shown to be located just outside the active site, possibly modulating the function of the catalytic residues to facilitate reactions with specific types of β-lactams. Most significant was the discovery of a likely disulfide bond in a large group composed of α-, β- and γ-proteobacteria that would contribute to enzyme stability and hence bacterial viability under antibiotic assault. A network of co-evolving residues was identified which suggested the importance of maintaining a surface for binding a highly conserved Phe69.     

Mass spectrometry of atmospheric aerosols--recent developments and applications. Part I: Off-line mass spectrometry techniques

Many of the significant advances in our understanding of atmospheric particles can be attributed to the application of mass spectrometry. Mass spectrometry provides high sensitivity with a fast response time to probe chemically complex particles. This review focuses on recent developments and applications in the field of mass spectrometry of atmospheric aerosols. In Part I of this two-part review, we concentrate on off-line mass spectrometry techniques, which require sample collection on filters but can provide detailed molecular speciation. In particular, off-line mass spectrometry techniques utilizing tandem mass spectrometry experiments and high resolution mass analyzers provide improved insight into secondary organic aerosol formation and heterogeneous reaction pathways through detailed structural elucidation at the molecular level.     

Thermal Conductivity of a Particulate-Diamond-Reinforced Cordierite Matrix Composite

The effect of 15 vol% particulate diamond reinforcement on the thermal conductivity of a cordierite matrix was studied as a function of diamond particle size from room temperature to 700°C. The thermal conductivity was found to increase with increasing particle size to a maximum increase of about 75% for a mean particle size of 50 μ. The particle size effect was found to be more pronounced at the lower temperatures than at the higher temperatures. The observed effect of particle size and temperature was attributed to the existence of an interfacial thermal barrier, possibly resulting from interfacial phonon scattering, with a positive temperature dependence of the interfacial thermal conductance. The magnitude of this conductance suggested strong adhesion between the diamond and cordierite.     

Experimental Characterization of Micro-Friction on a Mica Surface Using the Lateral Motion and Force Measurement Capability of an Instrumented Indenter

An experimental characterization of friction forces between asymmetric surfaces in the micro-regime is presented. The lateral motion and force-measurement capability of an instrumented indenter (triboindenter) is characterized and explored for determining friction properties at low velocities. Friction experiments are performed using the triboindenter with high repeatability. It is observed that real-time depth measurements closely follow the Hertzian prediction. Friction spikes with magnitudes depending on the drive velocity input are observed with peak friction force increasing with the dwell time. Anisotropy is observed between surfaces of different materials with stick-slip occurring only at specific relative orientations. Directions for expanding the current range of the triboindenter to obtain data from the nano to the macro scale are also presented.     

Sources and temporal dynamics of arsenic in a New Jersey watershed, USA

We examined potential sources and the temporal dynamics of arsenic (As) in the slightly alkaline waters of the Wallkill River, northwestern New Jersey, where violations of water-quality standards have occurred. The study design included synoptic sampling of stream water and bed sediments in tributaries and the mainstem, hyporheic-zone/ground water on the mainstem, and seasonal and diurnal sampling of water at selected mainstem sites. The river valley is bordered by gneiss and granite highlands and shale lowlands and underlain by glacial deposits over faulted dolomites and the Franklin Marble. Ore bodies in the Marble, which have been mined for rare Zn ore minerals, also contain As minerals. Tributaries, which drain predominantly forested and agricultural land, contributed relatively little As to the river. The highest concentrations of As (up to 34 mug/L) emanated from the outlet of man-made Lake Mohawk at the river's headwaters; these inputs varied substantially with season--high during warm months, low during cold months, apparently because of biological activity in the lake. Dissolved As concentrations were lower (3.3 microg/L) in river water than those in ground water discharging into the riverbed (22 microg/L) near the now-closed Franklin Mine. High total As concentrations (100-190 mg/kg) on the <0.63 microm fraction of bed sediments near the mine apparently result from sorption of the As in the ground-water discharge as well as from the As minerals in the streambed. As concentrations in river water were diluted during high stream flow in fall, winter and spring, and concentrated during low flow in summer. In unfiltered samples from a wetlands site, diurnal cycles in trace-element concentrations occurred; As concentrations appeared to peak during late afternoon as pH increased, but Fe, Mn, and Zn concentrations peaked shortly after midnight. The temporal variability of As and its presence at elevated concentrations in ground water and sediments as well as streamwater demonstrate the importance of (1) sampling a variety of media and (2) determining the time scales of As variability to fully characterize its passage through a river system.     

Effect of watershed parameters on mercury distribution in different environmental compartments in the Mobile Alabama River Basin, USA

Total mercury (THg) and mono-methylmercury (MeHg) levels in water, sediment, and largemouth bass (LMB) (Micropterus salmoides) were investigated at 52 sites draining contrasting land use/land cover and habitat types within the Mobile Alabama River Basin (MARB). Aqueous THg was positively associated with iron-rich suspended particles and highest in catchments impacted by agriculture. Sediment THg was positively associated with sediment organic mater and iron content, with the highest levels observed in smaller catchments influenced by wetlands, followed by those impacted by agriculture or mixed forest, agriculture, and wetlands. The lowest sediment THg levels were observed in main river channels, except for reaches impacted by coal mining. Sediment MeHg levels were a positive function of sediment THg and organic matter and aqueous nutrient levels. The highest levels occurred in agricultural catchments and those impacted by elevated sulfate levels associated with coal mining. Aqueous MeHg concentrations in main river channels were as high as those in smaller catchments impacted by agriculture or wetlands, suggesting these areas were sources to rivers. Elevated Hg levels in some LMB were observed across all types of land use and land cover, but factors such as shallow water depth, larger wetland catchment surface area, low aqueous potassium levels, and higher Chl a concentrations were associated with higher Hg burdens, particularly in the Coastal Plain province. It is suggested that the observed large variability in LMB Hg burdens is linked to fish displacement by anglers, differences in food web structure, and sediment biogeochemistry, with surficial sediment iron oxides buffering the flux of MeHg from sediments to deeper water pelagic food webs.