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31.
Montmorillinite clays, modified with either Zn(II) or Fe(III) chlorides have been used to catalyze the Friedel-Crafts alkylation of thiophene. High yields (ca. 80%) of 2- and 3-benzylthiophene mixtures were obtained using either catalyst in chlorobenzene or nitrobenzene solvent at 80°C. The ratio of 2–3-products was found to be controlled by their relative stability. Reactions were most rapid in nitrobenzene solution (<5 min) and resulted in formation of the 2-isomer as the only mono-substituted product. This is believed to be the first example in which an alkylation of thiophene has been limited to one regio-isomer. 相似文献
32.
Heat-shock induction of heat-shock protein genes is due to a specific promoter element (the heat-shock element, HSE). This study used lacZ under HSE control (HSE-lacZ) to characterize HSE activity in Saccharomyces cerevisiae cells of different physiological states and differing genetic backgrounds. In batch fermentations HSE-lacZ induction by heat shock was maximal in exponential growth, and showed marked decline with the approach to stationary phase. Expression in the absence of heat shock was unaffected by growth phase, indicating that the growth-dependent expression of many yeast heat-shock genes uses promoter elements in addition to the HSE. Heat-induced expression was strongly influenced by the temperature at which cultures were grown. While basal, uninduced expression was constant during growth at different temperatures to 30 degrees C, induction by transfer to 39 degrees C was reduced by increases in growth temperature as low as 18-24 degrees C. Maximal HSE-lacZ induction (30- to 50-fold) was in cultures grown at low temperatures (18-24 degrees C), then heat shocked at 39 degrees C. Ethanol was a poor inducer. Mutations having little effect on HSE-lacZ expression included a respiratory petite; ubi4 (which inactivates the poly-ubiquitin gene); also ubc4 and ubc5 (which each inactivate one of the ubiquitin ligases involved in degradation of aberrant protein). pep4-3 increased both basal and induced beta-galactosidase about two-fold, probably because of slower turnover of this enzyme in pep4-3 strains. 相似文献
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JH Noseworthy P O'Brien BJ Erickson D Lee D Sneve GC Ebers GP Rice A Auty WJ Hader A Kirk P Duquette J Carter G Francis L Metz E Shuster 《Canadian Metallurgical Quarterly》1998,51(5):1342-1352
OBJECTIVE: To determine whether sulfasalazine is better than placebo in slowing disability progression in MS. METHODS: In this randomized, double-blind, placebo-controlled phase III trial, 199 patients with active relapsing-remitting (n = 151) or progressive (n = 48) MS were evaluated at 3-month intervals for a minimum of 3 years (94% completed 3 years of follow-up; mean follow-up, 3.7 years). MRI studies were performed at 6-month intervals on a subset of 89 patients. RESULTS: Sulfasalazine failed to slow or prevent disability progression as measured by the primary outcome (confirmed worsening of the Expanded Disability Status Scale [EDSS] score by at least 1.0 point on two consecutive 3-month visits). Sulfasalazine influenced favorably a number of secondary outcomes during the first 18 months of the trial (e.g., annualized relapse rate, proportion of relapse-free patients; progressive subgroup only: rate of EDSS progression at 1 and 2 years, median time to EDSS progression) but these positive findings were not sustained into the second half of the trial. CONCLUSIONS: Sulfasalazine does not prevent EDSS score progression in the subset of MS patients studied by this protocol. Treatments may improve relapse-related outcomes in MS, at least temporarily, without providing sustained slowing of EDSS progression. Phase III MS trials should be of sufficient length to determine a meaningful impact on disease course. 相似文献
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Warren D. Smith 《Algorithmica》1992,7(1):137-177
This paper has two purposes. The first is to present a new way to find a Steiner minimum tree (SMT) connectingN sites ind-space,d >- 2. We present (in Appendix 1) a computer code for this purpose. This is the only procedure known to the author for finding Steiner minimal trees ind-space ford > 2, and also the first one which fits naturally into the framework of backtracking and branch-and-bound. Finding SMTs of up toN = 12 general sites ind-space (for anyd) now appears feasible.We tabulate Steiner minimal trees for many point sets, including the vertices of most of the regular and Archimedeand-polytopes with <- 16 vertices. As a consequence of these tables, the Gilbert-Pollak conjecture is shown to be false in dimensions 3–9. (The conjecture remains open in other dimensions; it is probably false in all dimensionsd withd 3, but it is probably true whend = 2.)The second purpose is to present some new theoretical results regarding the asymptotic computational complexity of finding SMTs to precision .We show that in two-dimensions, Steiner minimum trees may be found exactly in exponential time O(C
N
) on a real RAM. (All previous provable time bounds were superexponential.) If the tree is only wanted to precision , then there is an (N/)O(N)-time algorithm, which is subexponential if 1/ grows only polynomially withN. Also, therectilinear Steiner minimal tree ofN points in the plane may be found inN
O(N) time.J. S. Provan devised an O(N
6/4)-time algorithm for finding the SMT of a convexN-point set in the plane. (Also the rectilinear SMT of such a set may be found in O(N
6) time.) One therefore suspects that this problem may be solved exactly in polynomial time. We show that this suspicion is in fact true—if a certain conjecture about the size of Steiner sensitivity diagrams is correct.All of these algorithms are for a real RAM model of computation allowing infinite precision arithmetic. They make no probabilistic or other assumptions about the input; the time bounds are valid in the worst case; and all our algorithms may be implemented with a polynomial amount of space. Only algorithms yielding theexact optimum SMT, or trees with lengths (1 + ) × optimum, where is arbitrarily small, are considered here. 相似文献
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Several issues involved in creep feed grinding of alumina with diamond wheels were studied. Influences of variables on responses were investigated. Off-line optimization was performed by formulating the grinding process as a multi-objective nonlinear mixed integer problem. The dominant mechanism of material removal was determined. The grinding process was simulated to obtain the number of active cutting edges and the grinding parameters such as cutting length, maximum undeformed chip thickness, etc. Unit load distribution was characterized and then used to estimate the percentage of cutting edges that fracture or plastically cut the material and to derive the probability distribution function for substance cracks. Fractal concept was applied to characterize wheel profiles. Findings from these studies are reported in this paper. These studies are definitely not complete, but surely shed some light on the creep feed grinding process of ceramic materials. 相似文献
40.
Hung C. Ling Man F. Yan Warren W. Rhodes 《Journal of the American Ceramic Society》1991,74(2):287-289
The aging behavior of a series of lead perovskite dielectrics with the compositions x Pb(Fe2/3 W1/3 )O3 ·(1 – x )Pb(Fe1/2 -Nb1/2 )O3 , where 0 ≤ x ≤ 1, and the effect of dopants were studied. Below the Curie temperature ( T c ), the capacitance and the dissipation factor (tan δ) decrease approximately linearly with logarithmic time. The aging rate depends on the temperature difference, Δ T , between the aging temperature and T c , and on the dopant concentration, but is independent of the measurement frequency between 1 and 1000 kHz. The maximum aging rate is about 3% per decade of time for capacitance and 5% per decade for tan δ at 1 mol% dopant concentration, and increases to 6.3% for capacitance and 8.5% for tan δ at 0.7 mol% dopant concentration. These results are consistent with an aging mechanism caused by changing ferroelectric domain structure with time, as proposed for BaTiO3 . 相似文献