Characterization of elastic and time-dependent deformations in normal strength and high performance concrete by image analysis

Deformations in normal strength concrete (NSC) and high performance concrete (HPC) were examined using image analysis to better understand the distribution of strain in these materials as related to their composition and microstructure. Elastic strain, creep and shrinkage were shown to occur non-uniformly throughout the NSC and HPC microstructure and creep and shrinkage strain both increased with time, as expected. The non-uniformity in the strain measurements also increased with time, as a consequence of dissimilarities in time-dependent behavior of the paste and the aggregate. However, compared with NSC, the time-dependent strains in the HPC were lower and showed less variation, suggesting a more uniform microstructure. In both NSC and HPC, high-strain sub-regions were evident in the vicinity of the interfacial transition zone (ITZ), likely as a consequence of the strain mismatch between aggregate and paste. The thickness of the high strain sub-regions along the ITZ in HPC were approximately one half of those in NSC.     

New method for determination of absorption capacity of internal curing agents

Accurate assessment of absorption capacity (k) of internal curing agents is necessary to properly proportion cement-based mixtures and to measure their effectiveness in mitigating autogenous shrinkage. Standard methods for quantifying absorption capacity, such as those for coarse and fine aggregate, are not appropriate for the highly absorptive, finely divided materials often used for internal curing. Here, it is demonstrated that the absorption capacity of internal curing materials may be determined from early age heat evolution data measured through isothermal calorimetry. An example application, using pulp fibers as internal curing agents, is used to demonstrate the utility of the method.     

Investigation on nanocrystalline copper-doped zirconia thin films for optical sensing of carbon monoxide at high temperature

Nanocrystalline copper-doped zirconia (CDZ; Cu:Zr = 16:84) thin films have been synthesized on long-period fiber gratings (CDZ-LPFG) by a polymeric precursor method. The CDZ-LPFG device was demonstrated to have high sensitivity and good reversibility for low-concentration CO sensing at high temperatures. The CDZ-LPFG responds with red shifts of its resonant wavelength (λ_R) to CO-containing gases and the λ_R shift reverses when it is exposed to air. The optical response of the CDZ-LPFG to CO is due primarily to the CDZ refractive index variations resulted from the reversible redox reactions (i.e. Cu²⁺ ⇔ Cu⁺) in reducing and oxidizing atmospheres. The magnitude of the λ_R shift exhibited a strong dependence on CO concentration in a range from 0 to 1000 ppm that is potentially useful for quantitative measurement.     

1536孔板的洗涤：评价一种新型的双列吸液系统

基面效价测定（如酶联免疫及基于细胞的实验）通常需要程序洗板（1536孔）,这就使得一些高通量筛选实验室由于缺乏可靠的设备而无法常规进行这种实验。目前的1536孔板洗板机是采用8支针同时孔内加液,单列32支吸头从孔内一次性吸取液体,每次一列。事实上,这种多步操作严重地影响了实验效率。经统计,如果测定中包含洗板步骤,每24h的测试量为100板,而除去此限速步骤,相同时间内的测定量可增至500。因此,我们发明了一种64支吸头双列吸液装置用以弥补这种差距。结果表明此方法有效地提高了工作效率,并同样获得了较好的实验数据。     

Characterization of entrained air voids in cement paste with scattered ultrasound

This research develops a technique that uses the attenuation of ultrasonic waves to characterize the average size and volume fraction of entrained air voids in hardened cement paste. Quantitative knowledge of entrained air void size and distribution helps ensure that an adequate design strength is developed, while maintaining resistance to freeze-thaw damage in cement-based materials. Ultrasonic attenuation coefficients obtained from pulse-burst signals are measured in the frequency range of 500 kHz–5 MHz. From these parameters, the average size and the volume fraction of the entrained air voids are determined using a combination of an ultrasonic scattering model and an inversion algorithm. Experiments are performed on specimens produced with and without entrained air voids. There is a good agreement between the model prediction and the experiments in these systems that contained <10% by volume of entrained air voids.     

Detection of charged particles with charge injection devices

A method for using charge injection devices (CIDs) for detection of high-energy charged particles from inertial-confinement fusion reactions is described. Because of the relatively small depletion region of the CID camera (depletion depth of approximately 7 mum), aluminum foils are placed in front of the device to reduce the energy of the charged particles and maximize the energy deposited in the CID. Simultaneous measurements of (2)H(d,p)(3)H protons with a CID and a surface barrier detector indicate that the CID is an efficient detector of charged fusion products. Tests using high energy alpha particles emitted from a radium-226 source are also reported.     

Nicotinic agonists competitively antagonize serotonin at mouse 5-HT3 receptors expressed in Xenopus oocytes

Leptin is an adipocyte-derived blood-borne satiety factor that decreases food intake and increases energy expenditure, thereby leading to a substantial decrease in body weight. To explore the possible roles of the hypothalamic melanocortin system in leptin action, we examined the effects of intracerebroventricular (i.c.v.) injection of leptin with or without SHU9119, a potent antagonist of alpha-melanocyte stimulating hormone, on food intake, body weight, and mitochondrial uncoupling protein-1 (UCP-1) mRNA expression in the brown adipose tissue (BAT) in rats. A single i.c.v. injection of leptin decreased cumulative food intake and body weight gain, and increased UCP-1 mRNA expression during 3 h at the onset of the dark phase. Inhibition of food intake and body weight change with leptin was reversed by co-injection of SHU9119 in a dose-dependent manner. Co-injection of SHU9119 also inhibited completely the leptin-induced increase in UCP-1 mRNA expression in the BAT. Treatment with SHU9119 alone did not affect food intake, body weight, and UCP-1 mRNA expression in rats. The present study provides evidence that the hypothalamic melanocortin system plays a central role in both satiety effect and sympathetic activation of leptin.     

Nanoparticles and Apparent Activation Energy of Portland Cement

Although chemically inert nanosize mineral fillers have been shown to modify early cement hydration kinetics, with the effects dependent upon usage rate, particle size, and dispersibility, the effects of such fillers on the “apparent activation energy” (E_a) of cement has not been previously examined. Here, cement E_a was calculated from isothermal calorimetry performed at different temperatures with two different types of fillers (i.e., titanium dioxide and limestone) using a linear method as well as a modified ASTM C1074 method. The use of both types of nanoparticles increased the rate of cement hydration as well as accelerated the reaction rate, due to heterogeneous nucleation effect, as previously demonstrated. E_a increased in the presence of nanosized fillers, demonstrating an increased temperature sensitivity of the filler‐cement composites relative to ordinary cement. These results show that chemically inert nanoparticles behave fundamentally differently compared with supplementary cementitious materials such as fly ash and silica fume which instead decrease temperature sensitivity. The increased temperature sensitivity could thus be used to modify and optimize the reaction mechanism and kinetics of cement hydration, especially to increase the rate of cement hydration, to decrease setting time, and to achieve faster strength gain accounting for higher or lower temperatures during curing.     

Combined Phosphatase and Tensin Homolog (PTEN) Loss and Fatty Acid Synthase (FAS) Overexpression Worsens the Prognosis of Chinese Patients with Hepatocellular Carcinoma

We aimed to investigate the expression pattern of phosphatase and tensin homolog (PTEN), to evaluate the relationship between PTEN expression and clinicopathological characteristics, including fatty acid synthase (FAS) expression, and to determine the correlations of PTEN and FAS expression with survival in Chinese patients with hepatocellular carcinoma (HCC). The expression patterns of PTEN and FAS were determined using tissue microarrays and immunohistochemistry. The expression of PTEN was compared with the clinicopathological characteristics of HCC, including FAS expression. Receiver operator characteristic curves were used to calculate the clinical sensitivity and specificity of PTEN expression. Kaplan-Meier survival curves were constructed to evaluate the correlations of PTEN loss and FAS overexpression with overall survival. We found that the loss of PTEN expression occurred predominantly in the cytoplasm, while FAS was mainly localized to the cytoplasm. Cytoplasmic and total PTEN expression levels were significantly decreased in HCC compared with adjacent non-neoplastic tissue (both, p < 0.0001). Decreased cytoplasmic and total PTEN expression showed significant clinical sensitivity and specificity for HCC (both, p < 0.0001). Downregulation of PTEN in HCC relative to non-neoplastic tissue was significantly correlated with histological grade (p = 0.043 for histological grades I-II versus grade III). Loss of total PTEN was significantly correlated with FAS overexpression (p = 0.014). Loss of PTEN was also associated with poor prognosis of patients with poorly differentiated HCC (p = 0.049). Moreover, loss of PTEN combined with FAS overexpression was associated with significantly worse prognosis compared with other HCC cases (p = 0.011). Our data indicate that PTEN may serve as a potential diagnostic and prognostic marker of HCC. Upregulating PTEN expression and inhibiting FAS expression may offer a novel therapeutic approach for HCC.