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961.
P Oroszlan S Wicar G Teshima S L Wu W S Hancock B L Karger 《Analytical chemistry》1992,64(14):1623-1631
This paper examines the retention behavior of recombinant DNA-derived human growth hormone (rhGH) in reversed-phase chromatography and its separation from the closely related N-methionyl variant (Met-hGH). It is first shown that retention for rhGH decreases with increasing column temperature when 1-propanol (1-PrOH) is used as organic modifier. On the other hand, retention increases with temperature when acetonitrile (CH3CN) is employed. The differences in behavior for the two organic modifiers could be related to conformational changes in the protein as determined by solution and adsorption intrinsic fluorescence spectroscopy. Specifically, desorption and elution of rhGH using 1-PrOH could be correlated with a solvent-induced conformational change, with retention decreasing with increasing temperature due to the increasing ease of structural alteration. On the other hand for CH3CN the increase in retention correlated with temperature rise was related to a partial structural change yielding a more hydrophobic species. In this case, a surface-driven process is suggested. The work then turned to the separation of rhGH and Met-hGH where it was found for both organic modifiers optimum separation occurred at 45 degrees C and pH 6.5. Separate studies revealed that during the conformational change Met-hGH appeared more hydrophobic than rhGH since protein-protein aggregation was observed at a lower 1-PrOH concentration. It is suggested that this hydrophobic difference, which was optimized under the conditions cited above, resulted in the separation. The study demonstrates the importance of conformational changes in retention behavior and separation of protein samples. 相似文献
962.
The authors identify the coil parameters that are of importance, when assessing interturn voltages produced by steep-fronted transients. The studies are based on a multiconductor transmission line computer model of a machine winding energized by a simple ramp function. Variations in interturn voltages produced by changes in insulation thickness, insulation permittivity, turn geometry, coil length and number of turns are examined and certain guidelines for avoiding high interturn voltages are evaluated. An application study is included to show how interturn voltages can be significantly reduced by applying these guidelines 相似文献
963.
964.
Corticotropin-releasing factor (CRF) has previously been shown to selectively dilate the mesenteric vascular bed, without affecting other vascular beds. Pancreatic blood flow and islet blood flow were therefore measured separately with a microsphere technique in the two regions of the rat pancreas perfused by the superior mesenteric artery (SMA) or celiac artery (CA) respectively. Intravenous infusion of CRF (0.25 microgram/kg b.w./min) caused an increase in both whole pancreatic blood flow and islet blood flow in the region of the pancreas perfused by the SMA. The fraction of whole pancreatic blood flow diverted through the islets in this part of the pancreas was, however, unaffected by CRF infusion (approximately 10%). CRF did not change either pancreatic or islet blood flow in the CA-perfused part of the pancreas, and did not affect the release of insulin. 相似文献
965.
L. H. Yang Randolph Q. Hood J. E. Pask J. E. Klepeis 《Journal of Computer-Aided Materials Design》2007,14(3):337-347
High-Z metals constitute a particular challenge for large-scale ab initio electronic-structure calculations, as they require high resolution due to the presence of strongly localized states and require
many eigenstates to be computed due to the large number of electrons and need to accurately resolve the Fermi surface. Here,
we report recent findings on high-Z metals, using an efficient massively parallel planewave implementation on some of the largest computational architectures
currently available. We discuss the particular architectures employed and methodological advances required to harness them
effectively. We present a pair-correlation function for U, calculated using quantum molecular dynamics, and discuss relaxations
of Pu atoms in the vicinity of defects in aged and alloyed Pu. We find that the self-irradiation associated with aging has
a negligible effect on the compressibility of Pu relative to other factors such as alloying.
The US Goverment’s right to retain a non-exclusive, royalty-free license in and to any copyright is acknowledged. 相似文献
966.
L. Renaud F. Fouquet J. P. Millet H. Mazitle J. L. Crolet 《Materials and Manufacturing Processes》1991,6(2):315-330
It is possible to realize surface alloys by laser melting an electroless nickel layer containing chromium carbide particles predeposited on a mild steel substrate. By this way the surface alloy is expected to have not only a high nickel content but also an important chromium content in order to improve the corrosion resistance. The presence of chromium in solid solution results from the dissolution or melting of the carbide particles. Typical laser solidification microstructures are obtained. Dendrites consist of an austenitic Fe-Ni-Cr solid solution and interdendritic regions are constituted by an eutectic mixture containing the same austenitic solid solution and complex Fe, Ni, Cr carbides and phosphides. In comparison with a surface alloy obtained by laser melting of an electroless nickel layer without carbide particles, the corrosion resistance was slightly improved in saline aqueous solutions. The limited effect was due to the fact that the final chromium content in the present experimental conditions was not as high as that initially expected. 相似文献
967.
A novel soft-switching full-bridge DC/DC converter: analysis,design considerations, and experimental results at 1.5 kW, 100 kHz 总被引:1,自引:0,他引:1
The addition of an external commutating inductor and two clamp diodes to the phase-shifted PWM (pulsewidth modulated) full-bridge DC/DC converter substantially reduces the switching losses of the transistors and the rectifier diodes, under all loading conditions. The authors give analyses, practical design considerations, and experimental results for a 1.5 kW converter with 60 V, 25 A output, operating at 100 kHz clock frequency and 95% efficiency 相似文献
968.
969.
L. Bonci M. Gattobigio G. Iannaccone M. Macucci 《Journal of Computational Electronics》2002,1(1-2):49-53
We present a Monte Carlo simulation of two implementations of Quantum Cellular Automaton (QCA) circuits: one based on simple ground state relaxation and the other on the clocked cell scheme that has recently been proposed by Tóth and Lent. We focus on the time-dependent behavior of two basic circuits, a binary wire and a majority voting gate, and assess their maximum operating speed and temperature requirements for different sets of fabrication parameters. 相似文献
970.
S. R. Syrtsov V. N. Shut D. A. Il'yushchenko E. L. Gavrilenko 《Journal of Engineering Physics and Thermophysics》2002,75(5):1229-1233
The influence of the parameters of posistor elements and the conditions of their heat exchange on the electrical and dynamic characteristics of a posistor block have been investigated. Good agreement between calculation results and experimental data has been established. 相似文献