Constitutive expression of terminal deoxynucleotidyl transferase in transgenic mice is sufficient for N region diversity to occur at any Ig locus throughout B cell differentiation

N region diversity in Ag receptors is a developmentally regulated process in B and T cells that correlates with the differential expression of terminal deoxynucleotidyl transferase (TdT). Absent in fetal and newborn mice, TdT expression is restricted to early T and pro-B cells in adults. To extend the TdT expression pattern throughout B cell ontogenesis, we generated transgenic mice carrying a TdT cDNA under the regulatory elements of the N-myc gene and the IgH enhancer. High expression was observed in secondary lymphoid organs consistent with TdT activity beyond the pre-B cell stage. This suggests that TdT transgene expression is not down-regulated as is the endogenous gene. Unlike normal mice, extensive N region diversity was found in rearranged lambda light chain genes of adult transgenic animals. Therefore, expression of TdT appears sufficient for N region diversity to occur at any Ig locus. More importantly, expression of the transgene takes place during fetal development. As a consequence, the potential fetal B cell repertoire is modified as both rearranged heavy and light chain genes now show N region additions. Constitutive expression of TdT throughout B cell differentiation does not therefore appear deleterious and suggests that TdT is recruited only to participate in the V(D)J recombination process.     

不同压力下的热压加工对含Mn块体纳米晶Fe3Al组织和性能的影响

对铝热反应熔化制备的含10wt%Mn块体纳米晶Fe3Al材料在800℃和1000℃温度下,采用不同压力进行热压加工,利用XRD研究热压加工前后纳米晶粒尺寸的变化,并测试硬度变化和压缩率。结果显示,不同温度、压力下热压加工后,晶粒尺寸变化不大;热压加工后,材料的硬度有所上升,但温度和压力对硬度影响不大;热压加工中,材料表现出较好的塑性变形,并随着热压压力的增大,材料压缩率逐渐增大,但温度对材料的压缩率影响不明显。热压加工后,块体纳米晶Fe3Al材料几何尺寸得到了增大。     

关于定量界定设备修理周期若干问题的研究

本文通过对确定设备修理周期经验公式的讨论,指出了两点不足。对此笔者提出了:在生产过程中运用单位产品维修成本与产量的量化模型,从经济效益的角度动态界定最佳修理周期的设想,以期在设备维修管理方面,为企业提供最佳经济效益。并由此得出了几点有益的启示。     

利用开关卡尔曼滤波器的目标跟踪技术研究

为了使机器人视觉伺服控制系统的目标跟踪精度得到进一步提高,构建一种基于开关卡尔曼滤波器的视觉伺服控制系统。研究视觉伺服的目标跟踪原理,推导相关的数学模型,并分析跟踪误差产生的原因;针对图像采集和处理引入的延时问题,通过卡尔曼滤波估计得到目标运动的速度信息,以此作为前馈量输入视觉伺服控制器,补偿由于目标运动和延时造成的跟踪误差;为了解决卡尔曼滤波器由于目标运动的突然变化而降低估计性能的问题,引入运动监视器以在目标运动突然变化时发出开关信号并重置卡尔曼滤波器;最后对该算法进行实验与仿真。结果表明:基于卡尔曼滤波器的视觉伺服控制器能把跟踪误差控制在1 mm以内,而开关卡尔曼滤波器能有效地减少因目标运动状态突然变化而产生的跟踪误差。     

蛋白质、蛋白酶以及短肽类的分离提纯技术及应用

介绍了蛋白质、蛋白酶以及短肽类研究的一些传统方法和新兴方法。主要对分离、提纯、鉴定及分析方法的使用条件、适用范围、操作步骤、各自特点和应用价值作了简要阐述,并对有关方法进行了比较与评价。     

EMULSION COATING TO EXTEND POSTHARVEST LIFE OF MANGO (MANGIFERA INDICA CV. MANILA)

A coating emulsion was prepared using maltodextrins, carboxymethylcellulose, propileneglycol and a mixture of s orbit an fatty acid esters with an HLB of 6. The emulsion was sprayed on fully mature, unripened manila mangoes, which were the stored at 15 and 25C and 80–85% R.H. At 25C it was observed that rate CO² production increased and lost in control fruits after 12 days of storage. Coated mangoes kept their rate CO² production and suffered only 8% weight loss after 21 days of storage. After storage, the coating was washed out and fruits were allowed to ripen naturally, which occurred in 3–4 days. The results obtained from this study indicate that application of the hydrophobic coating extended the postharvest storage of mangoes for at least 20 days more than uncoated fruits.     

Gastric trichobezoar in a banner-tailed kangaroo rat (Dipodomys spectabilis)

A male, wild-caught kangaroo rat developed anorexia and wasting. The animal was euthanized and a gastric trichobezoar found at necropsy. The paucity of information regarding the clinical medicine of this species is a hindrance to those charged with the care of kangaroo rats. Gastric trichobezoar should be considered as a differential diagnosis in cases of anorexia in kangaroo rats.     

The toxicological problems of disaster medicine in the Navy Fleet]

From position of the uniform concept of the accident medicine the problems of toxicological supply of the ships of MNF are considered. Peculiarities of chemical accidents in the ship conditions are marked. The question about principles of harmful chemical substances regulating, forming air environment of ship living quarters is discussed. The prospects of practical use of antidotal means, intended for protection of personnel from toxic action of carbon dioxide and chemical combinations, causing development of toxic edema of lungs are given.