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91.
Inhibition of HeLa cell messenger RNA translation by 7-methylguanosine 5'-monophosphate 总被引:2,自引:0,他引:2
LA Weber ER Feman ED Hickey MC Williams C Baglioni 《Canadian Metallurgical Quarterly》1976,251(18):5657-5662
Translation of HeLa cell RNA containing poly(A) in a wheat germ cell-free system is markedly but incompletely inhibited by 7-methylguanosine 5'-monophosphate (m7G5'p). We have analyzed the translation products synthesized in the presence of different concentrations of m7G5'p and find that translation of all mRNAs is equally inhibited. To demonstrate the specificity of the inhibitor for RNAs with 5'-terminal m7G5' ppp... we show that specific translation products of satellite tobacco necrosis virus RNA, which does not have this 5' terminus, are synthesized in the presence of m7G5' p. Protein synthesis programmed by endogenous mRNA in a HeLa cell-free system is inhibited after a 10-min lag by m7G5' p. Other guanosine nucleotides without the 7-methyl group or with the phosphate in a different position are not inhibitor. We show that translation of all mRNAs is inhibited to a similar extent by m7G5'p in the HeLa cell-free system, by synthesizing 35S-labeled proteins in the presence of different inhibitory concentrations of this nucleotide and analyzing the translation products by electrophoresis and autoradiography. Translation of encephalomyocarditis virus RNA added to the HeLa cell-free system is not inhibited by m7"g5p; this viral RNA does not have this nucleotide at the 5' terminus. This indicates that m7G5'p specifically inhibits translation of mRNAs with the 5' terminus m7G5'ppp... and suggests that initiation of translation of picornavirus RNA may proceed via a mechanism different from that of cellular mRNAs. 相似文献
92.
The smoking habits of 56 families who lost babies to the sudden infant death syndrome (SIDS) were compared to those of 86 control families. A higher proportion of SIDS mothers smoked both during pregnancy (61% vs. 42%) and after their babies were born (59% vs. 37%). SIDS mother also smoked a significantly greater number of cigarettes than controls. Exposure to cigarette smoke ("passive smoking") appears to enhance the risk of SIDS for reasons not known. 相似文献
93.
Because of the unresolved controversy regarding the effect of epidural anesthesia upon uterine contractility, it was decided to study its effect on a small number of patients. Intrauterine and intra-arterial continuous pressure, continuous fetal heart rate, and maternal heart rate recordings were obtained from at least 20 minutes before administration of the epidural anesthic until complete dilatation in these patients. Nineteen patients were in spontaneous labor, and 18 had labor stimulated with oxytocin. Plain lidocaine, 1 or 1.5%, was used in 12 patients (30 observations), and lidocaine with epinephrine, 1:200,000 was used in 26 patients (51 observations). Uterine contractions were calculated in Montevideo units for 60 minutes following the epidural anesthetic. The changes, if any, were compared in both groups. There was a significant decrease in uterine activity when epinephrine was added to the anesthetic solution, mainly a lessening of intensity. There were comparable decreases in systolic/diastolic blood pressure in both groups and compensatory tachycardia. In one case, severe hypertension was observed following administration of lidocaine epinephrine. It was concluded that the addition of epinephrine to the anesthetic solution predictably produces diminution of uterine activity, and it does not give "cardiovascular support" to the laboring patient. 相似文献
94.
VA Belitser TV Varetskaia SN Tsynkalovskaia LA Tsariuk LI Shevchenko 《Canadian Metallurgical Quarterly》1976,48(4):521-532
A new diagnostic method to determine fibrinogen and fibrin splitting products is based on the fact that high-molecular fragments acting as specific inhibitors of fibrin self-assembly compose these products. Fibrinogen and fibrin splitting products are detected and determined quantitatively by prolongation of coagulation time in the test with the standard monomeric fibrin. The number of units of anticoagulating activity in the studied urine are the results of the determinations. Sensitivity of the method if necessary may be increased by adding of a certain amount of the fibrinogen and fibrin splitting products to the test that makes a favourable background for the action of the products. The method is rather sensitive, reliable and simple. It may be used in ordinary clinical laboratories. The method proved to be quite satisfactory when testing renal diseases at the clinic. It is of great significance for early detection of the transplanted kidney rejection. The new method is compared with the known immunological method which consist in determination of the inhibitory effect of the fibrinogen and fibrin splitting products on agglutination of erythrocytes of specific antibodies; the erythrocytes being subjected to "tanning" and "loading" with fibrinogen. A satisfactory correlation is found between the results of two methods. 相似文献
95.
Stability analysis is of great significance in those feedback control systems in which the power amplifier is operated as a pulse-modulator device, since under these circumstances the whole control system is highly non-linear. Stability in PWM feedback control systems with a proportional type regulator has been amply described in the literature. Only recently, however, have such studies been extended to include systems with a proportional-plus-integral regulator. In this paper the problem is considered for the case involving a PWM control system, where the regulator is a proportional-plus-integral-plus-derivative, the PWM modulator is of a very general type and the controlled process is of arbitrary order. The stability of the system is analysed by means of a discrete version of the second Lyapunov method ; this method in turn leads to an investigation of the positivity region of a quadratic form defined in the parameter space of the regulator. To improve the stability region obtained, a procedure is investigated whereby only a few parameters of the quadratic Lyapunov function need to be varied. The method proposed has proved extremely simple and flexible to apply, even in cases where the system is of arbitrary order. 相似文献
96.
M. DE LA SEN 《控制论与系统》2013,44(2-3):125-150
Abstract A perturbation theory for evaluation algorithms of arithmetic expressions is applied to the generalized least-squares identification of the continuous parameters by using nonperiodic discrete data. The sampling instants are distributed in such a way that a sequence of the elemental arithmetic expressions of the identification algorithm are suboptimized according to bang-bang rules on the extrema of appropriate variation intervals. The weighting scalars in the generalized least-squares method are updated with the same purpose. 相似文献
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99.
Progress towards a methodology for high throughput 3D reconstruction of soot nanoparticles via electron tomography
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The aim of this work is to make progress towards the development of 3D reconstruction as a legitimate alternative to traditional 2D characterization of soot. Time constraints are the greatest opposition to its implementation, as currently reconstruction of a single soot particle takes around 5–6 h to complete. As such, the accuracy and detail gains are currently insufficient to challenge 2D characterization of a representative sample (e.g. 200 particles). This work is a consideration of the optimization of the steps included within the computational reconstruction and manual segmentation of soot particles. Our optimal process reduced the time required by over 70% in comparison to a typical procedure, whilst producing models with no appreciable decrease in quality. 相似文献
100.
针对机器人关节柔性引起的轨迹跟踪误差问题和末端执行器残余振动现象,在PD反馈控制律基础上,提出一种基于柔体动力学模型的前馈力矩补偿控制算法和后置多模态自适应输入整形算法前馈力/位混合控制策略。建立六轴工业机器人柔体动力学简化模型,在不附加关节编码器和外设的情况下进行柔体动力学参数辨识,再将动力学模型改写为力矩计算方程,经计算得到前馈力矩并加入控制律中进行补偿;计算前馈力矩所用的期望轨迹需经过输入整形器处理,考虑机器人系统的时变性问题,采用后置多模态自适应输入整形算法对期望输入位移信号进行命令整形,从而抑制末端的残余振动现象。结果表明:所提出的前馈力/位混合控制策略能有效减少轨迹跟踪误差,抑制机器人末端的残余振动现象。 相似文献