Inhibition of reaper-induced apoptosis by interaction with inhibitor of apoptosis proteins (IAPs)

IAPs comprise a family of inhibitors of apoptosis found in viruses and animals. In vivo binding studies demonstrated that both baculovirus and Drosophila IAPs physically interact with an apoptosis-inducing protein of Drosophila, Reaper (RPR), through their baculovirus IAP repeat (BIR) region. Expression of IAPs blocked RPR-induced apoptosis and resulted in the accumulation of RPR in punctate perinuclear locations which coincided with IAP localization. When expressed alone, RPR rapidly disappeared from the cells undergoing RPR-induced apoptosis. Expression of P35, a caspase inhibitor, also blocked RPR-induced apoptosis and delayed RPR decline, but RPR remained cytoplasmic in its location. Mutational analysis of RPR demonstrated that caspases were not directly responsible for RPR disappearance. The physical interaction of IAPs with RPR provides a molecular mechanism for IAP inhibition of RPR's apoptotic activity.     

Interactions of spatial frequency and unequal monocular contrasts in stereopsis

Increasing the contrast of just one eye's image degrades stereothresholds; this phenomenon is referred to as the stereo contrast paradox. In experiment one, this paradox was found to be absent in dynamic random-element stereograms; thresholds were simply limited by the lower of the two eyes' contrasts. In experiment two, in which narrowband Gabor targets were used, the paradox was found to be strongest at relatively low spatial frequencies (1 cycle deg-1). As spatial frequency was increased, the paradox gradually disappeared. At relatively high spatial frequencies (5 cycles deg-1), thresholds were generally limited by the lower of the two eyes' contrasts, as was found for the dynamic noise targets. These results demonstrate the interactions of spatial frequency and contrast in binocular image combination and yield clues as to the different roles which high and low spatial frequencies may play in stereopsis.     

Phase equilibria and surface tension in castor oil-based polyols-water–methanol mixture: Thermodynamic basis

Considering the current environmental issues with petro-polymers, castor oil is a promising green alternative to the polyols to be used as the main raw material for polyurethane. However, the basic information regarding the processability of castor oil-based polyol (CP) such as its miscibility in different solvents is still elusive. Herein we have investigated the phase equilibria and surface tension of various CP solutions using thermo-optical analysis and pendent drop method, respectively. The modified double lattice (MDL) model was employed to theoretically describe those phase equilibria and surface behaviors. The phase diagrams of CPs/methanol binary systems represent upper critical solution temperature (UCST) behaviors, and CPs/methanol/water ternary systems show Treybal Type 2 behaviors. The surface tension results exhibit preferential solvent adsorption, which means affinity of the solvent to be present in surface is higher than that of the polymer. The calculated results showed good agreement with the experimental observations.     

Safety Evaluation for Campus Parking Garage Performance Using Fuzzy Logic

Crime has been plaguing parking garages in the United States. At The Ohio State University, (OSU) crime on campus, in general, has decreased in recent years; however, crime in OSU parking garages has remained stable in spite of campus-wide efforts to reduce crime. When assessing and evaluating parking garages, occupants employ experience-based judgment that is subjective and imprecise, yet extremely useful. When designers employ the principles of crime prevention through environmental design, they also use subjective variables. An approach that captures the imprecise information in noncrisp expressions reflecting subjective and experience-based variables is the fuzzy logic concept. This concept allows the use of mathematical operations to manipulate and operate on imprecise information or knowledge. Variables such as garage illumination and wall height were collected, fuzzified, and treated as the knowledge base of a model presented in this paper. In addition, numerous experience-based implication rules were developed and employed to evaluate subjective variables. A computer program was developed to illustrate implementation of the model.     

Age-specific reference intervals for plasma vitamins A, E and beta-carotene and for serum zinc, retinol-binding protein and prealbumin for Sydney children aged 9-62 months

Paediatric reference intervals for blood concentrations of certain nutrients are often based on either adult data or are derived from small samples of young children. Biochemical data were obtained from 467 randomly selected, healthy preschool children aged 9-62 months in Sydney, Australia. Data were obtained for plasma vitamins A, E and beta-carotene and for serum zinc, retinol-binding protein and prealbumin. Reference intervals based on the 2.5 and 97.5 centiles for age groups 9-23, 24-35, 36-47, 48-62 months and for the total group (9-62 months) were calculated. The 2.5-97.5 centiles for the whole group were: vitamin A, 0.7-1.8 mumol/l (20.05-51.56 micrograms/dl); vitamin E, 8-30 mumol/l (0.34-1.29 mg/dl); beta-carotene, 0.1-1.1 mumol/l (5.4-59.0 micrograms/dl); zinc, 9-19 mumol/l (58.8-124.2 micrograms/dl); retinol-binding protein, 14-36 mg/l; prealbumin, 104-264 mg/l. The reference intervals reported are consistent with the findings of a number of smaller studies and are likely to be an accurate reflection of the true intervals for healthy preschool children in western developed countries.