Megasonic agitation for enhanced electrodeposition of copper

In this paper we propose an agitation method based on megasonic acoustic streaming to overcome the limitations in plating rate and uniformity of the metal deposits during the electroplating process. Megasonic agitation at a frequency of 1 MHz allows the reduction of the thickness of the Nernst diffusion layer to less than 600 nm. Two applications that demonstrate the benefits of megasonic acoustic streaming are presented: the formation of uniform ultra-fine pitch flip-chip bumps and the metallisation of high aspect ratio microvias. For the latter application, a multi-physics based numerical simulation is implemented to describe the hydrodynamics introduced by the acoustic waves as they travel inside the deep microvias.     

Durability of fire retardant treated wood products at humid and exterior conditions review of literature

Fire retardants may considerably improve the fire properties of wood products, but the durability, e.g. in exterior applications, has not been addressed fully. This paper reviews the existing knowledge and experience mainly from the USA with the aim of supporting further development in Europe. The review is concentrated on pressure impregnated fire retardant treated wood products which have the best opportunities for increased durability. Copyright © 2001 by John Wiley & Sons, Ltd.     

Europe as Consumer of Exotic Biodiversity: Greek and Roman times

Landscapes at the edge of the city comprise vast areas that ‘lie fallow’, awaiting future urban development. During this time new landscape values evolve. It is argued that the complexity of urban fringe landscapes is not adequately considered either within landscape research or in the practice of spatial planning. A key to understanding landscapes at the inner urban fringe is to focus on landscape dynamics, and on the interactions between spatial plans and everyday activities. The study is divided into three parts. First, theoretical considerations about landscape dynamics and the character of the inner urban fringe are presented. Thereafter, the relationship between spatial planning and everyday places is analysed in a case-study area at the edge of the city of Malmö in southernmost Sweden. The case study demonstrates the complexity of landscape dynamics at the inner urban fringe, as well as problems regarding the handling of ephemeral and transitory aspects within spatial planning. The study concludes with a discussion concerning the importance of studies of landscape dynamics within landscape research.     

Book reviews

RESOURCE TRENDS AND POPULATION POLICY: A TIME FOR REASSESSMENT, by Lester R. Brown. Worldwatch Institute, Washington, D.C., May 1979; Worldwatch Paper 29. (Paperback $2.00)

THE NEXT SEVENTY YEARS: POPULATION, FOOD AND RESOURCES, by B. Gil‐land. Abacus Press, London, 1979. 133 pp. (£9.50)

POPULATION POLICY ANALYSIS, edited by M. E. Kraft and M. Schneider. D. C. Heath, London, 1979. (£0.00)

ENVIRONMENTAL IMPACT ASSESSMENT, by Brian B. Clark, Ronald Bisset and Peter Wath‐ern. Manssell, London and Bowker, New York, 1980. 516 pp. (£0.00)

ADVANCES IN ECOLOGICAL RESEARCH, Vol. 11, edited by Amyan MacFadyen. Academic Press, London, 1980. 428 pp. (£25.00)

MICROBIAL GROWTH AND SURVIVAL IN EXTREMES OF ENVIRONMENT, edited by G. W. Gould and Janet E. Cory. Academic Press, London, 1980. 244 pp. (£16.00)

UNDER NEW MANAGEMENT: Port Growth and Emerging Coastal Management Programs, by Marc Hershman, Robert Goodwin, Andrew Ruot‐sala, Maureen McCrea and Yehuda Hayuth. A Washington Sea Grant Publication, 1978. 212 pp. ($12.50)

HANDBOOK OF INDUSTRIAL ENERGY ANALYSIS, by J. Boustead and G. F. Hancock. Ellis‐Harwood, Chichester, U.K., 1979. (£25.00)

TREE GROWTH AND ENVIRONMENTAL STRESSES, by T. T. Kozlowski. University of Washington Press, Seattle and London, 1979. vii + 192 pp. (£12.50)

ALL GOOD THINGS AROUND US, by Pamela Michael. Illustrated by Christabel King. Ernest Benn Ltd., London, 1980. 240 pp. (£12.95)

HUMAN GEOGRAPHY (2nd Edition), by A. V. Perpillou. Longman Group, London, U.K., 1977. xviii + 570 pp. 37 pls., 27 maps and diagrams, Index. (Paperback £5.50)

INDUSTRIAL AND HAZARDOUS WASTES IMPOUNDMENT, by N. P. Cheremisinoff, P. N. Cheremisinoff, F. Ellerbeck and A. J. Perna. Ann Arbor Science, Michigan, 1979. ix + 475 pp. Index. (£16.25)

PHOSPHORUS IN THE ENVIRONMENT: ITS CHEMISTRY AND BIOCHEMISTRY. (Ciba Foundation No. 57, New Series), by Ruth Porter and David W. Fitzsimons. Elsevier, 1978. (£18.15)

TOXIC AND HAZARDOUS WASTE DISPOSAL. Vol. II, edited by R. B. Pojasek (Options for Sterilization Solidifications). Ann Arbor Science, Michigan, U.S.A., 1979, 259 pp. (£19.80)

SON OF THE WILDERNESS (The Life of John Muir), by Linnie Marsh Wolfe. The University of Wisconsin Press, Madison, 1978. 364 pp. (Paperback £4.90)

NATURE'S PRICE, by W. van Dieren and M. G. W. Hummelinck. Marion Boyars, London, Boston, 1979. (Paperback £3.50)

PERMACULTURE ONE, by Bill (B. C.) Molli‐son and David Holmgren. Corgi Books, Trans‐world Publishers Ltd., Melbourne, Australia. 128 pp. (A $4.95)

ENVIRONMENTAL AERODYNAMICS, by R. S. Scorer. Ellis Horwood, Chichester, U.K., 1978. 488 pp. (Hardback £20.00; paperback £7.50)     

Computer-based engineering of thermostabilized antibody fragments

We used the molecular modeling program Rosetta to identify clusters of amino acid substitutions in antibody fragments (scFvs and scAbs) that improve global protein stability and resistance to thermal deactivation. Using this methodology, we increased the melting temperature (T_m) and resistance to heat treatment of an antibody fragment that binds to the Clostridium botulinum hemagglutinin protein (anti-HA33). Two designed antibody fragment variants with two amino acid replacement clusters, designed to stabilize local regions, were shown to have both higher T_m compared to the parental scFv and importantly to retain full antigen binding activity after 2 hr of incubation at 70°C. The crystal structure of one thermostabilized scFv variants was solved at 1.6 Å and shown to be in close agreement with the RosettaAntibody model prediction.     

Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice

Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs). PGs are essential for correct collagen fibrillogenesis and maintaining corneal homeostasis. We investigated the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs after a chemical injury. The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas. The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed. We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS. Expression of the CSPGs biglycan and versican was increased after AB, while decorin expression was decreased. We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression. No significant changes in HS composition were noted after AB. Taken together, our study reveals significant changes in the composition of the extracellular matrix following a corneal chemical injury.     

A structural approach to understanding the interactions between colour,water-holding capacity and tenderness

The colour, water-holding capacity (WHC) and tenderness of meat are primary determinants of visual and sensory appeal. Although there are many factors which influence these quality traits, the end-results of their influence is often through key changes to the structure of muscle proteins and their spatial arrangement. Water acts as a plasticiser of muscle proteins and water is lost from the myofibrillar lattice structure as a result of protein denaturation and consequent reductions in the muscle fibre volume with increasing cooking temperature. Changes in the myofilament lattice arrangement also impact the light scattering properties and the perceived paleness of the meat. Causes of variation in the quality traits of raw meat do not generally correspond to variations in cooked meat and the differences observed between the raw muscle and cooked or further processed meat are discussed. The review will also identify the gaps in our knowledge and where further investigation would beneficial.     

Relative abundance,age structure,and body size in mudpuppy populations in southwestern Ontario

Little is known of mudpuppy (Necturus maculosus) population structure and ecology; some populations in the Great Lakes are thought to be in decline. Mudpuppies are the obligate hosts for the mudpuppy mussel (Simpsonaias ambigua), a species that is endangered in Canada and in many Great Lakes states. We surveyed mudpuppies from the Sydenham River, the only known Canadian locality of the mudpuppy mussel, in order to generate information on relative density, deformity rates and population age/size structure and used this information to compare them to known mudpuppy populations from Great Lakes sites in the Detroit River, Lake St. Clair and Long Point in Lake Erie. Deformity rates were elevated at some sites in the Sydenham River. The relative density of mudpuppies in the Sydenham River was lower than that of other Great Lakes sites and their age was skewed towards younger individuals. Although at lower densities than at other Great Lakes sites, the mudpuppy population in the Sydenham River appears stable and is showing signs of recruitment which bodes well for the future of the mudpuppy mussel population of the river.     

An Innovative Distributed Project Control Approach: A Case Study of the Cassini Management Information System (CMIS)

ABSTRACT

The Cassini Project, a NASA-funded exploration mission to Saturn that is being managed by the California Institute of Technology's Jet Propulsion Laboratory for NASA, has developed a distributed project schedule control system for complex development programs. The system is called the Cassini Management Information System (CMIS). The system was built to make schedule control simple, user-friendly, and low-cost. CMIS is distributed across a local area network of PCs and Macintosh computers and consists of four major modules: schedule control, action item tracking, work package implementation plan, and budget tracking. This article focuses on the schedule control module of CMIS. CMIS was one of the innovations introduced in response to the decrees of the U.S. Congress, and subsequently NASA, to conduct space exploration on a fixed-price basis. This article summarizes CMIS development history as a schedule control tool, its key features, and its use by more than 130 engineers and managers.