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91.
92.
Hyperglycemia before ischemia worsens cerebral outcome. The aim of this study was to determine the cerebral effects of giving glucose with or without insulin after asphyxial cardiac arrest. Rats underwent 8 min of asphyxial cardiac arrest. After arrest, Group 1 received NaCl; Group 2, insulin; Group 3, glucose; and Group 4, glucose plus insulin, all intravenously. Neurological deficit (ND) scores were 14+/-10%, 22+/-12%, 12+/-10% and 2+/-2% in Groups 1-4, respectively, 72 h after reperfusion. Overall histological damage (HD) scores were 4, 2, 3 and 1, respectively. Group 4 fared significantly better than group 1 on both scores. Glucose after asphyxial cardiac arrest in rats produces no increased brain damage while glucose plus insulin improves cerebral outcome. 相似文献
93.
We report two patients where the finding of isolated fetal hydrocephalus led to the detection of severe fetal thrombocytopenia, using fetal blood sampling. Serological investigation led to the diagnosis of fetomaternal alloimmune thrombocytopenia (FMAIT) due to anti-HPA-1a. Both women had had previous unsuccessful pregnancies probably due to FMAIT; one had had four miscarriages at 17-18 weeks' gestation. The other had had one previous pregnancy complicated by severe fetal anaemia, and eventually hydrocephalus developed and the fetus died without the diagnosis of FMAIT being considered. Subsequent pregnancies in the two women were also affected by FMAIT, but prenatal treatment, predominantly with serial fetal platelet transfusions, resulted in a successful outcome in both cases. These observations suggest that FMAIT should be suspected if there is isolated fetal hydrocephalus, unexplained fetal anaemia, or recurrent miscarriages. The accurate diagnosis of FMAIT is important because recent advances in prenatal management can improve the outcome of subsequently affected pregnancies. 相似文献
94.
GP Xue JS Johnson DJ Smyth LM Dierens X Wang GD Simpson KS Gobius JH Aylward 《Canadian Metallurgical Quarterly》1996,45(1-2):120-126
Temperature-regulated expression of recombinant proteins in the tac promoter (Ptac) system was investigated. Expression levels of fungal xylanase and cellulase from N. patriciarum in E. coli strains containing the natural lacI gene under the control of the Ptac markedly increased with increasing cultivation temperature in the absence of a chemical inducer. The specific activities (units per milligram protein of crude enzyme) of the fungal xylanase and cellulase produced from recombinant E. coli strain pop2136 grown at 42 degrees C were about 4.5 times higher than those of the cells grown at 23 degrees C and were even slightly higher when compared with cells grown in the presence of the inducer isopropyl beta-D-thiogalactopyranoside. The xylanase expression level in the temperature-regulated Ptac system was about 35% of total cellular protein. However, this system can not be applied to E. coli strains containing lacIq, which confers over production of the lac repressor, for high-level expression of recombinant proteins. In comparison with the lambda PL system, the Ptac-based xylanase plasmid in E. coli pop2136 gave a considerably higher specific activity of the xylanase than did the best lambda PL-based construct using the same thermal induction procedure. The high-level expression of the xylanase using the temperature-regulated Ptac system was also obtained in 10-litre fermentation studies using a fed-batch process. These results unambiguously demonstrated that the temperature-modulated Ptac system can be used for overproduction of some non-toxic recombinant proteins. 相似文献
95.
Timing and causality in process algebra 总被引:4,自引:0,他引:4
There has been considerable controversy in concurrency theory between the ‘interleaving’ and ‘true concurrency’ schools.
The former school advocates associating a transition system with a process which captures concurrent execution via the interleaving
of occurrences; the latter adopts more complex semantic structures to avoid reducing concurrency to interleaving.
In this paper we show that the two approaches are not irreconcilable. We define a timed process algebra where occurrences
are associated with intervals of time, and give it a transition system semantics. This semantics has many of the advantages
of the interleaving approach; the algebra admits an expansion theorem, and bisimulation semantics can be used as usual. Our
transition systems, however, incorporate timing information, and this enables us to express concurrency: merely adding timing
appropriately generalises transition systems to asynchronous transition systems, showing that time gives a link between true
concurrency and interleaving. Moreover, we can provide a complete axiomatisation of bisimulation for our algebra; a result
that is often problematic in a timed setting.
Another advantage of incorporating timing information into the calculus is that it allows a particularly simple definition
of action refinement; this we present. The paper concludes with a comparison of the equivalence we present with those in the
literature, and an example system specification in our formalism.
Received December 20, 1993/February 23, 1995 相似文献
96.
97.
The effective thermal conductivity eff has been measured for 1.6 K for mixtures with 10–6–2. Both eff and the derived impurity mass diffusion coefficient Diso show an unexpected dependence on the height h of the fluid layer. A scaled representation of Diso leads to a purely phenomenological model involving an effective length scale, proportional to X–1 and temperature independent. The relations so obtained are consistent with the observations as well as with the observed transition curve QC(X) to non-linear behavior. 相似文献
98.
Jan Haas Karen S. Frese Farbod Sedaghat-Hamedani Elham Kayvanpour Rewati Tappu Rouven Nietsch Oguz Firat Tugrul Michael Wisdom Carsten Dietrich Ali Amr Tanja Weis Torsten Niederdrnk Michael P. Murphy Thomas Krieg Marcus Drr Uwe Vlker Jens Fielitz Norbert Frey Stephan B. Felix Andreas Keller Hugo A. Katus Benjamin Meder 《International journal of molecular sciences》2021,22(4)
With more than 25 million people affected, heart failure (HF) is a global threat. As energy production pathways are known to play a pivotal role in HF, we sought here to identify key metabolic changes in ischemic- and non-ischemic HF by using a multi-OMICS approach. Serum metabolites and mRNAseq and epigenetic DNA methylation profiles were analyzed from blood and left ventricular heart biopsy specimens of the same individuals. In total we collected serum from n = 82 patients with Dilated Cardiomyopathy (DCM) and n = 51 controls in the screening stage. We identified several metabolites involved in glycolysis and citric acid cycle to be elevated up to 5.7-fold in DCM (p = 1.7 × 10−6). Interestingly, cardiac mRNA and epigenetic changes of genes encoding rate-limiting enzymes of these pathways could also be found and validated in our second stage of metabolite assessment in n = 52 DCM, n = 39 ischemic HF and n = 57 controls. In conclusion, we identified a new set of metabolomic biomarkers for HF. We were able to identify underlying biological cascades that potentially represent suitable intervention targets. 相似文献
99.
High resolution XPS analysis of chemical functionalised multi-wall carbon nanotubes (MWCNTs) and single wall carbon nanotubes (SWCNT) was done with ESCA300 (overall instrument resolution of 0.35 eV). Information to the degree of functionalisation was ascertained by argon ion bombardment of the samples followed by XPS analysis to detect the functional groups, the percentage atomic concentration of various elements present and whether or not the detected functional groups imposed a chemical shift on the CNT atoms. The results show that true chemical functionalisation was achieved and by argon ion bombardment these functional groups can be altered relative to the C 1s carbon atoms of the CNT. The choice of chemicals used for functionalisation, the techniques employed and the types of nanotubes treated are important factors in chemical characterisation. The carbon atom on the nanotube ring to which the functional group (atom) is bonded, the chirality of the CNT, the electronegativity of the functional group, the bond type and whether the CNT is single-wall or multi-wall, or cut (short) could play a role in determining the chemical shift on the CNTs atoms. These investigations are relevant to chemical functionalisation of carbon nanotubes for various applications for example DNA sensors and other biomedical sensors. 相似文献
100.
T.I.T. Okpalugo P. Papakonstantinou H. Murphy J. Mclaughlin N.M.D Brown 《Carbon》2005,43(14):2951-2959
The surface compositional and any structural changes that occur on carbon nanotubes using air-atmospheric pressure dielectric barrier discharge (APDBD) for functionalization are investigated employing Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), thermal gravimetric analysis (TGA), X-ray diffraction (XRD), and neutron diffraction techniques. Atmospheric pressure plasmas (APP) are suggested to be particularly suitable for functionalization of aligned nanotubes, where wet chemical manipulation could damage or even destroy the highly desirable vertical alignment. In this work a detailed experimental study elucidating the effects of APDBD plasma treatment parameters (e.g. power density, discharge composition, inter-electrode gap and treatment time) on the electronic structure, physical, and chemical behaviour of carbon nanotubes has been conducted. In an atmospheric air we find an optimal oxidative functionalization of CNTs in our DBD system within few seconds (<5 s) at a discharge power of ∼0.5 kW. This investigation may find useful application as functionalization technique for CNT engineered devices and sensors. 相似文献