Pharmacological analysis of intracellular Ca2+ signalling: problems and pitfalls

Sexual coercion, a topic of relevance to school health personnel, may be as common in high school populations as in university populations. Twenty-one sexuality education curricula were examined for information on the topics of date rape, stranger rape, pressure, incest, sexual harassment, unwanted/inappropriate touch, and exploitation/victimization. Curricula scoring highest in total coverage also were the most comprehensive with six of the seven sexual coercion topics covered. Overall, pressure and exploitation/victimization received the greatest attention, while sexual harassment was not covered in any of the curricula. Common themes occurring within the coercion topic areas included guilt, communication/assertiveness skills, blame, drug use, premeditation, fear, sources of help. Results suggest sexuality education curricula have not responded to the increased concern regarding sexual harassment in schools.     

The effect of dark and equiluminant occlusion on the interocular transfer of visual aftereffects

Because changes in intracellular Ca2+ affect progression through the mitotic cell cycle, we investigated the role of Ca2+-binding proteins in regulating cell cycle progression. Evidence was found demonstrating that the activation of Ca2+/calmodulin-dependent protein kinase (CaM kinase) inhibits cell cycle progression in small cell lung carcinoma (SCLC) cells. We also demonstrated that SCLC cells express both CaM kinase type II (CaMKII) and CaM kinase type IV (CaMKIV). Five independent SCLC cell lines expressed proteins reactive with antibody to the CaMKII beta subunit, but none expressed detectable proteins reactive with antibody to the CaMKII alpha subunit. All SCLC cell lines tested expressed both the alpha and beta isoforms of CaMKIV. Immunoprecipitation of CaMKII from SCLC cells yielded multiple proteins that autophosphorylated in the presence of Ca2+ / calmodulin. Autophosphorylation was inhibited by the CaMKII(281-302) peptide, which corresponds to the CaMKII autoinhibitory domain, and by 1-[N,O-bis(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4- phenylpiperazine (KN-62), a specific CaM kinase antagonist. Influx of Ca2+ through voltage-gated Ca2+ channels stimulated phosphorylation of CaMKII in SCLC cells, and this was inhibited by KN-62. Incubation of SCLC cells of KN-62 potently inhibited DNA synthesis, and slowed progression through S phase. Similar anti-proliferative effects of KN-62 occurred in SK-N-SH human neuroblastoma cells, which express both CaMKII and CaMKIV, and in K562 human chronic myelogenous leukemia cells, which express CaMKII but not CaMKIV. The expression of both CaMKII and CaMKIV by SCLC cells, and the sensitivity of these cells to the anti-proliferative effects of KN-62, suggest a role for CaM kinase in regulating SCLC proliferation.     

What exactly is information warfare? — Part 1

This series of articles, based on the author's book Information Warfare, investigates the issues concerning businesses wishing to protect themselves from the very real possibility of information warfare. We consider what the problems are and how they can be combatted over the course of the next few issues of the newsletter. This particular article outlines the details of Class 1 Information Warfare.     

Bilayer segmentation of webcam videos using tree-based classifiers

This paper presents an automatic segmentation algorithm for video frames captured by a (monocular) webcam that closely approximates depth segmentation from a stereo camera. The frames are segmented into foreground and background layers that comprise a subject (participant) and other objects and individuals. The algorithm produces correct segmentations even in the presence of large background motion with a nearly stationary foreground. This research makes three key contributions: First, we introduce a novel motion representation, referred to as "motons," inspired by research in object recognition. Second, we propose estimating the segmentation likelihood from the spatial context of motion. The estimation is efficiently learned by random forests. Third, we introduce a general taxonomy of tree-based classifiers that facilitates both theoretical and experimental comparisons of several known classification algorithms and generates new ones. In our bilayer segmentation algorithm, diverse visual cues such as motion, motion context, color, contrast, and spatial priors are fused by means of a conditional random field (CRF) model. Segmentation is then achieved by binary min-cut. Experiments on many sequences of our videochat application demonstrate that our algorithm, which requires no initialization, is effective in a variety of scenes, and the segmentation results are comparable to those obtained by stereo systems.     

Behavior of perpendicular recording materials in Cl and S containing atmospheres

The corrosion behavior of Co-Cr and Ni-Fe alloys is reported after exposure to Cl2, SO2, and O2containing test atmospheres at various relative (RH) humidities. Surface Chemistry, physical features, and magnetic properties were characterized. The Ni-Fe alloys exhibited excellent corrosion resistance in elevated RH atmospheres. Co-Cr alloys showed little change in magnetic properties even when significant chemical penetration of the film occurred. Substrate chemical composition proved to be an important factor in sputtered film corrosion behavior.     

Lecture series — unconfined gas explosions,von Karman Institute for Fluid Dynamics,Rhode-Saint-Genèse,Belgium, February 7–11, 1983

The literature on the spread and vaporisation of cryogenic liquids on water is reviewed and a new model proposed. The model incorporates the features o

T001. List of symbols

     

57.

Limitations of data compiled from police reports on pediatric pedestrian and bicycle motor vehicle events   总被引：4，自引：1，他引：4  

P F Agran  D N Castillo  D G Winn 《Accident; analysis and prevention》1990,22(4):361-370

Police reports were compared to the information provided by a hospital monitoring system for children under 15 years old injured as pedestrians and bicyclists by moving motor vehicles in Orange County, California. The analysis was limited to identifying caveats in the police report database. Underreporting by police was conservatively estimated at 20% for pedestrians and 10% for bicyclists. Comparison of the pedestrian databases suggested underreporting by police of incidents involving 0-4-year-olds, nontraffic incidents, incidents in which the vehicle was backing up, and cases not involving a child crossing a street. Comparison of the bicyclist databases indicated an underreporting by police of nontraffic cases. These caveats, in part, are related to police agency reporting requirements. The police injury severity scale was found to correlate poorly with a scale based on medical diagnoses, and substantial underreporting by police of serious injuries was demonstrated. We suggest that utilization of police injury severity scales be limited to categories of fatal, injured, and not injured (when available).     

58.

Index of pretreatment intensity predicts outcome of high-dose chemotherapy and autologous progenitor cell transplantation in chemosensitive relapse of Hodgkin's disease     

LM Jost  L Widmer  HP Honegger  RA Stahel 《Canadian Metallurgical Quarterly》1997,8(8):785-790

PURPOSE: To identify prognostic factors in patients with chemosensitive relapsed Hodgkin's disease treated by high-dose chemotherapy with autologous progenitor cell transplantation (HDC) and to compare the duration of treatment-free remission prior to HDC with the progression-free survival after HDC in individual patients. PATIENTS AND METHODS: Forty-five consecutive patients were analyzed retrospectively. We devised an index of pretreatment intensity (IPTI) based number of different chemo- and radiotherapy regimens given between diagnosis and HDC and on the duration of disease. RESULTS: With a median follow-up of 47 months the post-transplant event-free survival (EFS) was 44% and the overall survival (OAS) was 62% at four years. The IPTI allowed to discriminate between a low and a high-risk group with a four-year post-transplant EFS of 66% and 11% and a OAS of 87% and 28%, respectively (P = 0.0001). Of the 39 patients with sufficient follow-up after HDC, post-transplant EFS lasted on average > or = 18.5 months longer than the pretransplant treatment-free remission. CONCLUSIONS: HDC with the CBV regimen confers significant benefit to patients with chemosensitive relapsed Hodgkin's disease. The IPTI may help to select patients with a good response to HDC and to identify poor prognosis patients suitable for experimental protocols or palliative care only.     

59.

Prediction of response during interferon alfa 2b therapy in chronic hepatitis C patients using viral and biochemical characteristics: a comparison   总被引：1，自引：0，他引：1  

MJ Tong  LM Blatt  JG McHutchison  RL Co  A Conrad 《Canadian Metallurgical Quarterly》1997,26(6):1640-1645

Patients with chronic hepatitis C (n = 103) were treated for 24 weeks with interferon alfa 2b and followed up for 24 weeks after cessation of therapy (week 48). When hepatitis C virus (HCV) RNA at week 48 was used to assess interferon response, 15 (14.6%) were virological complete responders, and all have remained HCV RNA negative for a mean of 3 years. At week 48, 3 of 15 virological complete responders had elevated alanine transaminase (ALT) values. When serum ALT level was used at week 48 to determine response to interferon, 20 (19.4%) were biochemical complete responders. However, 8 of the 20 patients with normal ALT levels were HCV RNA positive at week 48, and 7 of these individuals have had a recurrence of elevated ALT levels within 3 years after cessation of treatment. These findings indicate that measurement of HCV RNA was more accurate than ALT in determining true responses to interferon therapy. Identification of nonresponders early during the course of interferon treatment showed that an elevated ALT level at week 12 was 92% predictive (odds ratio 3.7) but misidentified 33% (5 of 15) of the patients who were virological complete responders at week 48. In contrast, a positive HCV RNA at week 12 of treatment was 98% predictive (odds ratio 35.5) and misidentified only 6.7% (1 of 15) of the virological complete responders. Thus, positive HCV RNA at week 12 of therapy was more accurate in identifying eventual virological nonresponders than measurement of ALT at this time. Termination of interferon therapy in patients who were HCV RNA positive at week 12 would result in a 27% reduction in the direct medical costs and keep patients from undergoing unnecessary treatment. Therefore, testing for HCV RNA at week 12 to identify nonresponders and then discontinuing their treatment is practical, cost-efficient and beneficial both to patients and to third-party payers.     

60.

Problems of differential diagnosis and prognosis of diabetes mellitus in adults     

GS Turiev  AS Ametov  VI Smirnova  LP Ivanova  VZ Topchiashvili  VV Nosikov  LM Demurov  II Kondratév  AV Timofeev 《Canadian Metallurgical Quarterly》1997,75(3):26-29

     

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Symbols
Cp	Heat capacity.
g	Acceleration due to gravity.
Gm	Molar vaporisation rate.
h	Latent heat with two suffices denoting phase change.
h	Pool thickness (no suffices).
i	Enthalpy.
k,K	Constants.
KA,KB	Functions of pool temperature and composition.
kw	Thermal diffusivity of water.
l	Depth in water where temperature is constant.
L	Length scale of an oil spill.
Lm	Molar liquid spill rate.
Lwi	Latent heat of fusion of ice.
M	Pool mass, M0 initially.
Ms,Mv	Mass spilled, mass vaporised.
q	Heat flux to cryogenic liquid.
r	Radial co-ordinate
R,R*	Radius of pool.
t	Time.
T	Temperature.
U	Velocity.
Wm	Number of moles of material in the pool.
xA	Molar methane portion of liquid pool (xB, ethane).
xAS	Molar methane portion of spilling liquid.
yA	Molar methane portion of vapour (yB, ethane).
z	Vertical coordinate.
δ	Vapour layer thickness.
ΔT	Temperature difference.
?	Ice layer thickness.
θ	Temperature relative to freezing point of water.
λ	Effective thermal conductivity.
μ	Viscosity.
?	Density.
σ	Surface tension.
ø	Heat flux to liquid pool = q.
Suffices
A	Average (or methane fraction with x,y).
B	Boiling point of cryogen (or ethane fraction with x,y).
fc	Film collapse.
fg	Liquid to vapour phase change.
F	Freezing of water.
i	Ice.
l, L	Liquid.
lv	Liquid to vapour phase change.
LE	Leading edge.
m	Molar.
O	Initial
o	Oil.
p	Pool.
s	Spilling liquid.
v	Vapour.
w	Water.

Limitations of data compiled from police reports on pediatric pedestrian and bicycle motor vehicle events

Police reports were compared to the information provided by a hospital monitoring system for children under 15 years old injured as pedestrians and bicyclists by moving motor vehicles in Orange County, California. The analysis was limited to identifying caveats in the police report database. Underreporting by police was conservatively estimated at 20% for pedestrians and 10% for bicyclists. Comparison of the pedestrian databases suggested underreporting by police of incidents involving 0-4-year-olds, nontraffic incidents, incidents in which the vehicle was backing up, and cases not involving a child crossing a street. Comparison of the bicyclist databases indicated an underreporting by police of nontraffic cases. These caveats, in part, are related to police agency reporting requirements. The police injury severity scale was found to correlate poorly with a scale based on medical diagnoses, and substantial underreporting by police of serious injuries was demonstrated. We suggest that utilization of police injury severity scales be limited to categories of fatal, injured, and not injured (when available).     

Index of pretreatment intensity predicts outcome of high-dose chemotherapy and autologous progenitor cell transplantation in chemosensitive relapse of Hodgkin's disease

PURPOSE: To identify prognostic factors in patients with chemosensitive relapsed Hodgkin's disease treated by high-dose chemotherapy with autologous progenitor cell transplantation (HDC) and to compare the duration of treatment-free remission prior to HDC with the progression-free survival after HDC in individual patients. PATIENTS AND METHODS: Forty-five consecutive patients were analyzed retrospectively. We devised an index of pretreatment intensity (IPTI) based number of different chemo- and radiotherapy regimens given between diagnosis and HDC and on the duration of disease. RESULTS: With a median follow-up of 47 months the post-transplant event-free survival (EFS) was 44% and the overall survival (OAS) was 62% at four years. The IPTI allowed to discriminate between a low and a high-risk group with a four-year post-transplant EFS of 66% and 11% and a OAS of 87% and 28%, respectively (P = 0.0001). Of the 39 patients with sufficient follow-up after HDC, post-transplant EFS lasted on average > or = 18.5 months longer than the pretransplant treatment-free remission. CONCLUSIONS: HDC with the CBV regimen confers significant benefit to patients with chemosensitive relapsed Hodgkin's disease. The IPTI may help to select patients with a good response to HDC and to identify poor prognosis patients suitable for experimental protocols or palliative care only.     

Prediction of response during interferon alfa 2b therapy in chronic hepatitis C patients using viral and biochemical characteristics: a comparison

Patients with chronic hepatitis C (n = 103) were treated for 24 weeks with interferon alfa 2b and followed up for 24 weeks after cessation of therapy (week 48). When hepatitis C virus (HCV) RNA at week 48 was used to assess interferon response, 15 (14.6%) were virological complete responders, and all have remained HCV RNA negative for a mean of 3 years. At week 48, 3 of 15 virological complete responders had elevated alanine transaminase (ALT) values. When serum ALT level was used at week 48 to determine response to interferon, 20 (19.4%) were biochemical complete responders. However, 8 of the 20 patients with normal ALT levels were HCV RNA positive at week 48, and 7 of these individuals have had a recurrence of elevated ALT levels within 3 years after cessation of treatment. These findings indicate that measurement of HCV RNA was more accurate than ALT in determining true responses to interferon therapy. Identification of nonresponders early during the course of interferon treatment showed that an elevated ALT level at week 12 was 92% predictive (odds ratio 3.7) but misidentified 33% (5 of 15) of the patients who were virological complete responders at week 48. In contrast, a positive HCV RNA at week 12 of treatment was 98% predictive (odds ratio 35.5) and misidentified only 6.7% (1 of 15) of the virological complete responders. Thus, positive HCV RNA at week 12 of therapy was more accurate in identifying eventual virological nonresponders than measurement of ALT at this time. Termination of interferon therapy in patients who were HCV RNA positive at week 12 would result in a 27% reduction in the direct medical costs and keep patients from undergoing unnecessary treatment. Therefore, testing for HCV RNA at week 12 to identify nonresponders and then discontinuing their treatment is practical, cost-efficient and beneficial both to patients and to third-party payers.