Effects of Transcendental Meditation versus resting on physiological and subjective arousal.

On 4 successive days, 10 highly trained and experienced meditators (aged 26–54 yrs) were asked to relax for 5 min, meditate for 20 min, and then relax for 5 min. In contrast, 10 other Ss (aged 22–32 yrs) who had no training or experience with meditation were asked to relax for 5 min, rest for 20 min, and then relax for 5 min. Physiological arousal (heart and respiration rates, skin resistance, and blood pressure) and subjective arousal (cognitive, somatic, and relaxation) were measured throughout the experiment. Prior to meditating or resting, meditators tended to have higher heart rates and diastolic blood pressure than did nonmeditators. Meditation was associated with generally reduced arousal; but while meditating, meditators did not evidence lower levels of arousal than nonmeditators did while resting. Results place qualifications on previous studies of the effects of meditation on arousal. Implications for the study of personality functioning, stress management, and psychotherapy are discussed. (27 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Endotoxic activity of cell-free rumen fluid from cattle fed hay or grain

The cell-free rumen fluid from cattle fed hay or grain exhibited the following biological characteristics which strongly suggest the presence of endotoxin or a toxic principle similar to endotoxin of gram-negative bacteria: proved lethal to mice when injected with actinomycin D; proved extremely lethal to chick embryo; induced biphasic pyogenic response in rabbits; enhanced susceptibility to bacterial infection in mice; evoked positive epinephrine skin reaction in rabbits and phenol-water or aqueous ether proved lethal to mice and chick embryos. A quantitative difference in concentrations of endotoxin was observed on LD50 in mice and chick embryos and response to the epinephrine skin test in rabbits. Cell-free rumen fluid of grain-fed cattle contained at least twice as much endotoxin as that of hay-fed cattle. Endotoxin in cell-free rumen fluid and in higher concentration in cattle fed grain than in those fed hay support the hypothesis that rumen bacterial endotoxins may participate in the pathogenesis of diseases associated with high grain feeding such as lactic acidosis and the sudden-death syndrome.     

S-adenosylmethionine metabolism and DNA methylation in hydrazine-treated rats

The treatment of rats with hepatotoxic doses of hydrazine (NH2-NH2) induces the rapid formation of 7-methylguanine and O6-methylguanine in liver DNA. The methyl moiety in these reactions might be derived from the cellular S-adenosylmethionine pool because radioactivity administered to these rats as methionine rapidly appears in the DNA as methylated guanine. An increased incorporation of radioactivity into 5-methylcytosine was previously reported followed by subsequent suppression. This increased radiolabeling of 5-methylcytosine coincided with time of maximal DNA guanine methylation. To determine the nature of S-adenosylmethionine metabolism during the period of DNA methylation induced by hydrazine treatment, and to determine if the increased radiolabeling of 5-methylcytosine at this time reflected an actual increase in 5-methylcytosine synthesis, liver DNA synthesis and S-adenosylmethionine levels and turnover were assayed. Liver S-adenosylmethionine concentrations varied slightly between control rats and hydrazinetreated rats during the first five hours after hydrazine administration, and no difference was detectable in the incorporation of administered [3H]methionine into S-adenosylmethionine. Because S-adenosylmethionine specific radioactivity in hydrazine-treated rats was not different from control rats, the previously observed increased radiolabeling of 5-methylcytosine appeared to represent an actual increase in synthesis. This conclusion was supported by finding that incorporation of radioactive thymidine into DNA was also accelerated immediately following hydrazine administration, again followed by a decrease. 5-Methylcytosine sythesis, therefore, appears to follow DNA synthesis during hydrazine toxicity, and formation of 7-methylguanine and O6-methylguanine in liver DNA of hydrazine-treated rats occurs during a short period of increased DNA sythesis and 5-methylcytosine formation very early in hydrazine toxicity.     

Air caloric test: irrigation technique

The technique of using air to perform the caloric test is discussed. Greater temperature differences and larger volumes of air are needed to achieve stimulations equal to those caused by water irrigations. Special consideration must be given to the design and operation of air irrigation equipment. The effects of irrigating a wet canal and of misdirecting the air jet are discussed. A new air irrigation apparatus is described.     

Field tests of a radon progeny sampler for the determination of effective dose

A two-screen sampler (an effective dosimeter), with a collection efficiency matched to the particle size response of the radon progeny dose conversion factors (DCF), obtained from the ICRP respiratory tract model as implemented in the computer code RADEP, has been developed to assess the inhalation dose from exposure to radon progeny. In order to evaluate the performance of this sampler, the second stage of a six-stage wire screen diffusion battery was designed to operate as an Effective Dosimeter. This hybrid system allowed two methods for the determination of the radon progeny DCF. For the first method, the activity size distributions, measured using the diffusion battery, were used to obtain a size-weighted DCF. A second determination of DCF was obtained directly from the fraction collected by the Effective Dosimeter. The hybrid diffusion battery was used to measure radon progeny in the Fairy Cave, Buchan, Victoria at 20-min intervals over a 30-h period. This cave had radon concentrations exceeding 2000 Bq m(-3), with low aerosol concentration and low ventilation rates. The measurements were analysed for the radon progeny PAEC, the activity size distribution, the size-weighted DCF and the effective dosimeter collected fraction. The Effective Dosimeter DCFs were determined from the collected fraction using firstly a simple linear function and then using a more complex polynomial function to correct for residual errors. For the linear factor alone, the calculated Effective Dosimeter DCFs were on average 11% lower than the equivalent size-weighted DCF values. The agreement using the polynomial function was improved markedly, with a linear regression of the DCF yielding a fitted ratio of 0.965, with an R value of 0.99. For this study, the use of the ICRP conversion convention to estimate the occupational exposure to the tour guides working in the Fairy Cave would under estimate the effective dose by up to a factor of 2.