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61.
RB Belshe LS Richardson TJ Schnitzer DA Prevar E Camargo RM Chanock 《Canadian Metallurgical Quarterly》1977,24(1):8-12
ts-2, a temperature-sensitive and plaque morphology mutant of respiratory syncytial virus and sole representative of complementation group B, was compared with members of the other complementation groups of respiratory syncytial virus (group A [ts-1] and group C [ts-7]). ts-2 was found to be 10- to 1,000-fold more restricted in growth and ability to spread at restrictive temperatures (37, 38, and 39 degrees C) than at the permissive temperature (32 degrees C). In temperature shift-up experiments, the ts defect of ts-1 and other members of complementation group A was found to effect a late function that was required for at least 13 h in the replicative cycle. The ts lesion of ts-7 affected a function early in the replication cycle. In contrast, ts-2 was not temperature sensitive when studied by the shift-up technique. The discrepancy between the ts plaque property and failure to detect temperature sensitivity during the shift-up experiment was resolved when it was shown that ts-2 had a defect in adsorption or penetration or both at the restrictive temperature. Clonal analysis of revertant ts-2 showed a coordinate restoration of ts+ phenotype ans syncytium-forming capacity. It appears that ts-2 has a defect in a protein that is involved in adsorption and/or penetration of virus and is also responsible for cell fusion activity. 相似文献
62.
Plasma-chemical etching of silicon and silicon compounds is reviewed. It is shown that present data indicates that fluorine atoms and molecules are the main particles responsible for etching of silicon and that atomic fluorine is the main active particle for etching of silicon dioxide. Insufficient data exists to establish the contribution of charged and excited states to the etching of silicon and its compounds but oxygen atoms and molecules are seen to have a strong influence and fluorocarbon radicals can passivate the surface by forming polymer films. 相似文献
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LS Cherkasova GG Vereshchako TM Mironova VA Novik MIu Ta?ts GF Tsykhun VG Fomichenko 《Canadian Metallurgical Quarterly》1977,49(4):15-18
Intensity of glycolysis was studied under conditions of immobilization stress and adrenalectomy as well as with administration of adrenaline and hydrocortisone. The processes of aerobic glycolysis, activity of enzymes of the tricarboxylic acid cycle and mitochondria respiratory chain, content of total, free and protein-bound glycogen, glucose-I-phosphate, glucose-6-phosphate, pyruvate, alpha-ketoglutarate, cytrate and macroergs in the brain are shown to depend on the level of adrenal hormones producing a multilateral effect on a series of energy metabolism links. 相似文献
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L Zardi LS Vincent A Barbin R Montesano GP Margison 《Canadian Metallurgical Quarterly》1977,3(3-4):183-188
DNA repair was measured in human fibroblasts, mouse C3H 10 T 1/2 fibroblsts and rat hepatocytes by the non-semi-conservative incorporation of [3H]-TdR during DNA repair synthesis using liquid scintillation techniques. Confluent monolayers of these cells grown on cover slips were exposed to split doses (125 or 250 microgram/ml) of the mutagenic and carcinogenic alkylating agent MNU and DNA repair synthesis compared with that produced by a single dose (500 microgram/ml). No significant difference in DNA repair capacity was detected in the three cell lines treated with a single dose or split doses of MNU. 相似文献
69.
This paper presents a M6800 microprocessor based hardware monitor for measuring certain selected parameters of the EC-1030 computer system. The motivation for this work was to get an insight into the operation of the system under the present work load and use these results towards improvement and design studies. The three main classes of quantitative indices of computer system performance/productivity, responsiveness and use have been measured using hardware and software tools. This paper describes the hardware monitor design and discusses the measurements made. 相似文献
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