Josef Maria Bro?ek (1913-2004).

Presents an obituary and information about the life and accomplishments of Josef Maria Bro?ek. Born August 14, 1913, in the ancient town of Melnik, in central Bohemia, today the Czech Republic, Josef spent his childhood in Poland (Warsaw, 1913- 1915) and in Siberia (1915-1920). His education in Czechoslovakia culminated with a thesis on "Memory, Its Measurement and Structure" and a PhD awarded in June 1937. In the history of psychology, his lifetime project bore the title "Historiography of Psychology Around the World," and it covered about 20 geographical areas. Extensive attention was devoted to institutional and organizational developments as journals, academic settings, archives, museums, research groups, conferences, and institutes. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

塔里木油田水碳酸盐平衡系数与有机酸的意义

根据统计学规律,对塔里木盆地油田水进行了研究,阐明了该盆地油田水的碳酸盐平衡系数(简称为R_C)与有机酸的基本特征。从油田水的无机组分与有机组分的统计分析中发现了油田水的碳酸盐平衡系数与有机酸存在明显的负相关性,进一步论证了碳酸盐平衡系数和有机酸与油气聚集及油气性质的关系。碳酸盐平衡系数越低及低碳有机酸的含量越高,它们与油藏,尤其是与 轻质油或气藏的关系越密切。从两者的关系中初步了解到有机酸的溶蚀作用对油气藏中储层次生孔隙的形成应有较重要的影响。     

加长方钻杆在随钻中的应用

在推广复合导向钻井技术及新型PDC钻头施工过程中,常常出现动力钻具产生的反扭矩带动钻具逆时针旋转现象,使钻机大绳打扭,将水龙带、有线随钻电缆等缠绕在一起,导致电缆损坏,耽误生产以及导致井下复杂情况发生等严重后果。通过在有线随钻中利用方钻杆柱的方法,即在有线随钻中,根据井架类型,选择2根或3根自加工的方钻杆连接在一起,通过方钻杆和转盘的相互作用,控制螺杆钻具产生的反扭矩,从而避免钻具突然逆时针旋转情况的发生,较好地解决了上述难题,通过在冀中地区的推广应用,使得钻井速度大幅度提高,钻井事故相应地减少,实现了安全、高效钻进,获得了可观的经济效益。     

温州市龙湾段标准堤工程软土地基变形原位观测

温州市龙湾段标准堤地基土中普遍存在一层海积淤泥,其强度低,压缩性高,承载力低,不能满足标准堤填筑及水闸运行的要求。采用预压法进行地基处理,取得的地基沉降、水平位移、孔隙水压力等现场观测资料,总结分析了该段海积淤泥地基的变形规律和特点。并提供了有关的原型观测数据,其沉降曲线规律性较好。     

对地域通信网呼损的研究与仿真

本文通过对具有迂回路由的地域通信网呼损的研究与计算机仿真,得出迂回路由的存在使呼损大为降低的结论,从而增加了地域通信网的抗毁性能。     

基于WVD的防空雷达直升机信号特征分析

基于直升机目标旋转部件的调制模型，本文针对防空雷达提出了分析直升机信号特征的一种新方法——WVD法，并对直升机目标旋转部件调制回波信号在不同噪声情况下加以仿真分析，结果表明：用WVD分析雷达回波中直升机信号特征是一种非常有效的方法。     

Effect of alendronate treatment on the osteoclastogenic potential of bone marrow cells in mice

The expression of cytokeratins (CK), involucrin, vimentin, CD34, and alpha-smooth-muscle actin was studied in fetal and adult hair follicles. The first stage of the developing hair follicle is characterized by palisaded, elongated epithelial cells budding from the epidermal basal layer. These cells express CK5/6, CK14, CK17, CK19, and vimentin. During the following weeks of gestation, different structures in the developing hair follicle can be identified and characterized. The matrical cells display only CK19. The keratinocytes of the outer root sheath express CK5/6, CK14, CK17, CK19, and involucrin; those of the inner root sheath, CK4, CK18, and involucrin; those of the isthmus, the same profile as the ORS. In the infundibulum, the basal-layer keratinocytes express CK5/6, CK14, CK17, and CK19, whereas in the suprabasal layers CK1, CK4, CK10, CK14, and CK17 are seen. The adult hair follicle in anagen fails to express CK19 in the matrical cells and isthmus and both CK17 and CK19 in the infundibulum. These profiles of intermediate filaments and other markers appear to be potentially useful in categorizing neoplasms with apparent follicular differentiation.     

Purification of the cardiac sarcoplasmic reticulum membrane protein phospholamban from recombinant Escherichia coli

Phospholamban (PLN) was expressed in Escherichia coli as a protein fusion with glutathione S-transferase (GST). GST-PLN was mostly present in the insoluble protein fraction and accounted for approximately 50% of total insoluble protein. Attempts to suppress inclusion body formation or to use GST as an affinity-purification tag failed. A successful purification method is based on preparative SDS/PAGE and electrodialysis. From 1 g cells we typically purified 13.5 mg fusion protein with a PLN content of 2.8 mg. We genetically inserted an enterokinase (EK) protease site just in front of the PLN sequence and demonstrated the proteolytical liberation of PLN from the carrier protein. The approach described represents a substantial advancement in PLN expression and purification.