Impact of Rantes and MCP-1 chemokines on in vivo basophilic cell recruitment in rat skin injection model and their role in modifying the protein and mRNA levels for histidine decarboxylase

RANTES and related molecules, constitute the C-C class of chemokine supergene family and a group of cytokines produced by hematopoietic cells constitute the MCP-1 or C-X-C class. The roles of most of these chemokines are not well known, although members of the C-X-C family are inflammatory agents. Here, we report that intradermal injection of RANTES 10 ng/50 microL subcutaneously in the abdominal skin produced a strong inflammatory reaction, as evidenced by Evans blue dye, greater than FMLP (10(-6) mol/L) (approximately 57%); while MCP-1, 10 ng/50 microL was less effective than FMLP (10(-6) mol/L) (approximately 54%). Moreover, the histologic analysis of the cells stained with Toluidine blue (0.1%) were analyzed at a magnification of x40). RANTES 10 ng/50 microL and LPS produced higher numbers (142 +/- 11 and 193 +/- 21 of cells/200 mm2, respectively) of basophilic cell accumulation in the skin injection sites compared with FMLP (10(-6) mol/L) (127 +/- 14/200 mm2), while MCP-1 10 ng/50 microL was less effective (88 +/- 10/200 mm2). Electron microscopy (x13,800) studies of skin injection sites revealed that RANTES was chemoattractant for mast cells. In a Northern blot analysis from homogeneous tissue biopsy from the intradermal injection sites, RANTES was more potent than MCP-1 in increasing histidine decarboxylase (HDC) mRNA, the sole enzyme responsible for the production of histamine from histidine. Since PGD2 is formed by mast cells on cell activation, we also studied the effect of RANTES and MCP-1 on PGD2 production in inflamed tissue in vivo. RANTES (20, 10, and 5 ng) and MCP-1 (20, 10, and 5 ng) strongly stimulated PGD2, in a dose-dependent manner, with a potency rank order of RANTES (10 ng/mL) approximately two times greater than MCP-1 (10 ng/mL).     

Dust emission by powder handling: Influence of the hopper outlet on the dust plume

Dust generation in solids handling involving free fall of bulk materials and impacts on a stockpile can cause many problems in industry and be a great danger for operators health. This paper describes an experimental set up to characterize the dust plume formed in free fall of powders from a hopper and investigates the influence of various outlet geometries on the dust plume. For this purpose an image analysis technique was developed to quantify the characteristics of the dust plume.     

Mediators of change for multisystemic therapy with juvenile sexual offenders.

The mediators of favorable multisystemic therapy (MST) outcomes achieved at 12 months postrecruitment were examined within the context of a randomized effectiveness trial with 127 juvenile sexual offenders and their caregivers. Outcome measures assessed youth delinquency, substance use, externalizing symptoms, and deviant sexual interest/risk behaviors; hypothesized mediators included measures of parenting and peer relations. Data were collected at pretreatment, 6 months postrecruitment, and 12 months postrecruitment. Consistent with the MST theory of change and the small extant literature in this area of research, analyses showed that favorable MST effects on youth antisocial behavior and deviant sexual interest/risk behaviors were mediated by increased caregiver follow-through on discipline practices as well as decreased caregiver disapproval of and concern about the youth’s bad friends during the follow-up. These findings have important implications for the community-based treatment of juvenile sexual offenders. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Thermal Properties and Phase Transition of 2ZrO2●P2O5 Studied by In Situ Synchrotron X‐ray Diffraction

High‐temperature in situ synchrotron X‐ray powder diffraction experiments were carried out to investigate the phase transition mechanism of Zr₂P₂O₉ (2ZrO₂·P₂O₅ or “Z₂P”). Linear thermal expansion coefficients were calculated for the low‐temperature phase (α‐Z₂P) and the high‐temperature phase (β‐Z₂P) from temperature‐dependent changes in lattice parameters. The crystal structures of α‐ and β‐Z₂P were determined as a function of temperature by performing Rietveld crystal structure refinements. The structural changes at the phase transition are accompanied by an increase in the average atomic distance between neighboring Zr atoms. The occurrence of shorter metal–metal distances in α‐Z₂P is interpreted to result from stronger interactions between partly occupied valence orbitals of the d⁰ metal atoms. The bond valence method was used to calculate the valence sums of the atoms of α‐ and β‐Z₂P, respectively, considering also contributions resulting from covalently bonded atoms. As the bond strength between the metal atoms in Z₂P decreases with the transition into the high‐temperature phase, notably, the metal–metal interactions are regarded to constitute a prerequisite for the stabilization of the α‐phase over the energetically favored β‐phase.     

Controlling the structure of a porous polymer by coupling supercritical CO2 and single screw extrusion process

A study on the extrusion of Eudragit E100 was carried out using supercritical carbon dioxide (scCO₂) as plasticizer and foaming agent. ScCO₂ modifies the rheological properties of the material in the barrel of the extruder and acts as a blowing agent during the relaxation when flowing through the die. For experiments, a single‐screw extruder was modified to be able to inject scCO₂ within the extruded material. The aim is to determine a correlation between operating conditions and foam structure. The effect of three parameters was studied: the temperature in the die and in the metering zone, the screw speed, and the volumetric flow rate of CO₂. An increase in temperature enhances the expansion rate and the average pore diameter and appears to be the most significant parameter. The effect of CO₂ concentration is significant at small concentrations only: the higher the CO₂ concentration, the lower the pore density and the higher both the pore diameter and the expansion rate. The effect of the screw speed is tricky because a variation of this speed involves a decrease of CO₂ weight ratio. This study shows that the structure of the extrudates does not evolve with a coupling of screw speed increase and a subsequent CO₂ weight ratio decrease. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010