Aqueous photoelectrochemistry of hematite nanorod array

A unique nanostructured rod-like morphology of hematite (α-Fe₂O₃), designed with no grain boundaries, has been investigated for the aim of a direct splitting of water at the hematite/electrolyte interface. Photoelectrochemical properties were studied by steady-state measurements on electrodes with controlled morphology and film thickness in aqueous electrolyte. The hematite electrodes were able to generate incident photon-to-current efficiencies (IPCEs) of 8% by illumination through the substrate with a wavelength of 350 nm and a light intensity of 0.1 mW cm⁻² without any applied voltage.On the basis of light intensity studies, it is concluded that charge carrier recombinations due to the poor semiconductor properties in combination with slow oxidation kinetics at the hematite nanorods/electrolyte interface are the dominating problems. However, the high IPCE values obtained indicates that purpose-built nanorods of hematite is one significant way to strikingly lower the recombination rate of hematite material.     

Thermotolerance in Saccharomyces cerevisiae: the Yin and Yang of trehalose

What is neuroinformatics? What is the Human Brain Project? Why should you care? Supported by a consortium of US funding agencies, the Human Brain Project aims to bring to the analysis of brain function the same advantages of Internet-accessible databases and database tools that have been crucial to the development of molecular biology and the Human Genome Project. The much greater complexity of neural data, however, makes this a far more challenging task. As a pilot project in this new initiative, we review some of the progress that has been made and indicate some of the problems, challenges and opportunities that lie ahead.     

Optimal data acquisition in fMRI using prolate spheroidal wave functions

Functional magnetic resonance imaging (fMRI) is a technique that can be used to noninvasively study mental activity in a persons brain. fMRI has the potential to answer many interesting questions regarding the way the brain functions. Unfortunately, the drawback to fMRI studies, as they are traditionally performed, is that the temporal resolution is too low to effectively answer questions regarding what happens in an active region of the brain immediately following stimulation. Shepp and Zhang ( 2000 ) introduced a new method that could potentially lead to a significant increase in temporal resolution. Their method suggests a way to improve the time resolution in fMRI studies by sampling only a fraction of the points needed to recreate a full image. Instead of full image reconstruction, an optimal prolate spheroidal wave function filter is used to obtain a measurement over the total activity in a predefined region in the brain, B , at successive time points. The sampling region and filter are chosen in order to minimize the energy loss over the region of interest (ROI). The region they suggested to sample was chosen heuristically and corresponds to the scaled polar set of B . It was shown to be near optimal through extensive computer searches. In this article the optimal sampling region is found for the case when the ROI is circular or spherical and the sampling size is small. Based on this result, a new heuristic is introduced for other ROIs, which improves upon the results obtained using the polar set of B . © 2003 Wiley Periodicals, Inc. Int J Imaging Syst Technol 13, 126–132, 2003; Published online in Wiley Inter‐Science (www.interscience.wiley.com). DOI 10.1002/ima.10051     

Amyloid fibres of Sup35 support a prion-like mechanism of inheritance in yeast

Hydrogels were prepared from poly(vinyl alcohol) and chitosan in various blend ratios. The water contents of the hydrogels were in the range of 65 to 75 wt %. The attachment and growth of fibroblast cells (L-929) on the hydrogels were studied with a cell culture method. On the hydrogels with more than 15 wt % chitosan content, the attached cells were able not only to remain viable but also to proliferate. The relative cell attachment after incubation for 30 h increased with increasing chitosan content in the hydrogels. Cell attachment and growth on the hydrogel with 40 wt % chitosan content exceeded those on collagen, a widely-used mammalian cell culture substrate. The morphology of the cells attached onto the hydrogels with a lower chitosan content was spherical, but in hydrogels with more than 15 wt % chitosan content, the number of spindle-shaped cells increased with increasing chitosan content.     

Identification of SSF1, CNS1, and HCH1 as multicopy suppressors of a Saccharomyces cerevisiae Hsp90 loss-of-function mutation

Hsp90 functions in a multicomponent chaperone system to promote the maturation and maintenance of a diverse, but specific, set of target proteins that play key roles in the regulation of cell growth and development. To identify additional components of the Hsp90 chaperone system and its targets, we searched for multicopy suppressors of various temperature-sensitive mutations in the yeast Hsp90 gene, HSP82. Three suppressors were isolated for one Hsp90 mutant (glutamate --> lysine at amino acid 381). Each exhibited a unique, allele-specific pattern of suppression with other Hsp90 mutants and had unique structural and biological properties. SSF1 is a member of an essential gene family and functions in the response to mating pheromones. CNS1 is an essential gene that encodes a component of the Hsp90 chaperone machinery. The role of HCH1 is unknown; its sequence has no strong homology to any protein of known function. SSF1 and CNS1 were weak suppressors, whereas HCH1 restored wild-type growth rates at all temperatures tested to cells expressing the E381K mutant. Overexpression of CNS1 or HCH1, but not SSF1, enhanced the maturation of a heterologous Hsp90 target protein, p60(v-src). These results suggest that like Cns1p, Hch1p is a general modulator of Hsp90 chaperone functions, whereas Ssf1p likely is either an Hsp90 target protein or functions in the same pathway as an Hsp90 target protein.     

Remarks on control design with degree constraint

The purpose of this note is to highlight similarities and differences between two alternative methodologies for feedback control design under constraints on the McMillan degree of the feedback system. Both sets of techniques focus on uniformly optimal designs. The first is based on the work of Gahinet and Apkarian and that of Skelton , while the other is based on earlier joint work of the authors with C. I. Byrnes.     

Design and Performance of a 46-m-High MSE Wall

This paper focuses on the design and performance of a very tall mechanically stabilized earth (MSE) wall. Expansion of Seattle-Tacoma International Airport called for the construction of a third runway west of the two existing runways. A significant volume of compacted earth fill was required to raise the grade as much as 50 m to meet the level of the existing airfield. Nominal 2H:1V fill slopes were used where possible, but MSE retaining walls were used where fill slopes would have encroached into existing wetlands. Consequently a four-tier 46-m-tall MSE wall was constructed along a portion of the western edge of the embankment. Performance monitoring included strain gauge-instrumented reinforcing strips, inclinometer installations with sondex settlement rings, optical survey of the wall facing for vertical and lateral movements, and piezometers. This paper describes wall design issues, aspects associated with the instrumentation of the wall, and the observed performance. Monitoring indicates satisfactory performance of the MSE wall and compares reasonably well with predicted performance.