Isolation and identification of a sulfate reducing bacteria and sequence analysis of its dissimilatory sulfite reductase gene

A sulfate reducing bacteria was isolated from mining sewage of Daqing Oilfield by Hungate anaerobic technology. Physiological-biochemical analysis showed that the strain could utilize polyacrylamide as sole carbon and nitrogen source. The sequence analysis of 16S rDNA illustrated that the similarity of F8 and Desulfovibrio desulfuricans (AF192153) was 99%, and the similarity sequence of dissimilatory sulfite reductase gene (DSR) cloned from the strain and Desulfovibrio desulfuricans (AF273034) was 98%. Their phylogenitic analysis was basically anastomosed, and thus temporarily named as Desulfovibrio desulfuricans F8. The DSR cloned from F8 strain was 2740 bp in length consisting of three ORF, DSRA, DSRB and DSRD as a single operon (DSRABD) regulated by the same operator. DSRA contained typical conservative box of sulfate—sulfite reducing enzyme (SiteⅠand SiteⅡ), which could bind siroheme and [Fe4S4]. DSRB retained a [Fe4S4] binding site, with an uncomplimentary structure for siroheme binding. There was no conservative box in DSRD. Sequence analysis of DSR will provide a theoretical basis for quantitative detection, metabolic pathway modification through gene engineering, and sulfate reducing bacteria (SRB) suppression.     

Geological factors associated with unsuccessful exploration wells in the northern margin of the Qaidam basin

We investigated the geological factors associated with unsuccessful exploration wells in the northern margin of the Qaidam basin to better understand their cause. The structural situation, the hydrocarbon accumulation mechanism and unsuccessful well data collected from 1996 to 2005 were studied. The results show that the main geological factors associated with unsuccessful exploration wells are a lack of effective source rocks and a lack of effective traps, as well as the migration-accumulation conditions that exist in this area. The basin was reformed by Meso-Cenozoic tectonic evolution. Multi-stage tectonic activities have both positive and negative effects on hydrocarbon accumulation. Source rocks distribution, effective migration channels, effective traps and the tectonic evolution effects on hydrocarbons should be the key objects for further studies.     

Surface wear resistance properties of Ta＋N implanted W18Cr4V high speed steel

High-speed steel W18Cr4V is commonly used in industries such as blade and mould manufacturers because of its high level of hardness and toughness, red-hardness and resistance. Ion implantation is an effective method to improve the wear resis-tance of W18Cr4V. In our investigation, Ta and Ta+N ion implantation was performed on W18Cr4V high-speed steel. The surface properties after implantation were evaluated by measuring friction coefficients while the carbonyl phase of the surface was ana-lyzed by X-ray diffraction analysis. It was found that the friction coefficients of the treated samples were much lower. Samples implanted with Ta+N had a lower friction coefficients than samples implanted only with Ta. This can be attributed to the formation of a new chemical compound, Fe7Ta3, on both surfaces. An even harder chemical compound, Fe2N, was formed on both sttrfaces of Ta+N implanted samples.     

一种决策树算法的研究

SURPASS是一种增量式的处理数字数据的决策树算法,常被用于入侵检测和模式识别.它在准确率方面超过了一些决策树算法,它的另一个优越性表现在能够处理大小超过计算机内存的数据集.但是由于SURPASS要处理大数据集,它生成决策树耗费的时间比较长.针对这一问题,提出了一种指标并指出了指标的两种形式,使得在SURPASS生成的决策树生长的每一步,可对属性的指标值排序,保留指标值较小的属性,算法从被保留的属性集中选择最佳属性进行分割.这样每一步需要评价的属性的个数减少,或者说在每一步,少量的内存操作可代替大量的外存操作.     

爪形大分子CBC—NO的合成、表征及降滤性能研究

以1,3-丁二醇、柠檬酸为原料、甲苯为溶剂,在120℃油浴中反应,合成了柠檬酸-1,3-丁二醇-柠檬酸（CBC）爪状物小分子;然后,以甲基苯磺酸为催化剂,使CBC依次与环烷酸、十八醇分别在140℃和180℃油浴下接枝,合成了新型的爪形大分子柠檬酸-1,3-丁二醇-柠檬酸-环烷酸-十八醇（CBC—NO）。采用^1H—NMR及IR对合成的两种化合物进行了结构表征。结果表明,合成的CBC和CBC—NO与所设计的分子结构相吻合。用元素分析确定了CBC的化学组成为C16H22O14。合成的CBC—NO溶于有机溶剂,不溶于水。将CBC—NO按600μg／g的加剂量添加到不同的轻柴油中,柴油的冷滤点最大可降低8℃。     

Damage and deterioration mechanism and curing technique of concrete structure in main coal cleaning plants

Concrete structures in main coal cleaning plants have been rebuilt and reinforced in the coal mines of the Shanghai Da-tun Energy Sources Co. Ltd., the first colliery of the Pingdingshan Coal Co. Ltd. and the Sanhejian mine of the Xuzhou Mining Group Co. Ltd. In these projects, the operating environment and reliability of concrete structures in the main plants of the three companies were investigated and the safety of the structures inspected. Qualitative and quantitative analyses were made on the spe-cial natural, technological and mechanical environments around the structures. On the basis of these analyses, we discuss the long-term, combined actions of the harsh natural (corrosive gases, liquids and solids) and mechanical environments on concrete structures and further investigated the damage and deteriorating mechanisms and curing techniques of concrete structures in the main coal cleaning plants. Our study can provide a theoretical basis for ensuring the reliability of concrete structures in main coal cleaning plants.     

周期阵列压痕奇异应力场与开裂（PartⅡ：压痕开裂临界载荷）

周期阵列压痕方法是研究微观摩擦学的工具之一。由于周期阵列刚性压头压在半平面弹性体的表面时,会产生周期的Ⅰ型奇异压应力场,该奇异应力场的应力集中达到一定程度时,即达到临界条件时,该奇异应力场所在的边界将开裂。压痕边界的开裂问题是一个新型的断裂力学问题,通过边界移动的能量释放率的分析给出了压痕边界开裂能量释放率数学表征,并给出边界开裂的临界载荷和临界开裂角。     

刘庄煤矿11-2煤层顶、底板砂岩水特征与防治技术

初步探讨了刘庄煤矿11-2煤层顶、底板砂岩裂隙水的特征及其综合防治技术,为其它煤层及相邻矿井煤层顶、底板砂岩水的防治提供参考。     

奥运中心区超标降雨积水风险分析及对策研究

随着城市的发展,城市积水造成了越来越严重的问题.解决问题的关键就是分析超标降雨下的城市积水风险,以指导近期应急排水预案以及远景城市雨水系统改造方案的制定.由于北京市降雨量年内分配很不均匀,汛期暴雨又多表现为局地短历时的大暴雨甚至特大暴雨,造成城市道路（特别是立交桥区）积水问题频发,严重影响城市交通.在此背景下,从保障奥运安全召开的角度出发,以奥运中心区为研究对象,收集整理奥运中心区雨水系统各组成部分相关资料,建立奥运中心区雨水系统数据库,建立奥运中心区雨水系统模型,对超标降雨条件下奥运中心区的可能积水风险进行了分析,并以此为基础进行对策研究和实施效果评价.     

基于MATLAB语言的地震波动力特性分析方法

地震波频谱组成的研究主要是利用Fourier分析或小波理论,而地震波对建筑物动力反应的研究则是通过拟加速度反应谱来进行．现行分析方法的数值化过程必须通过计算机编程来实现,而采用常规的编程语言实现起来往往较为复杂．基于此,作者提出了基于MAT—LAB平台的分析方法,可以大大提高地震波动力特性分析的效率,便于分析结果的图形化输出,为工程结构抗震计算提供有益的参考．