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991.
Comparing stress ECG enhancement algorithms 总被引:2,自引:0,他引:2
Afonso V.X. Tompkins W.J. Nguyen T.Q. Michler K. Shen Luo 《IEEE engineering in medicine and biology magazine》1996,15(3):37-44
There are two predominant types of noise that contaminate the electrocardiogram (EGG) acquired during a stress test: the baseline wander noise (BW) and electrode motion artifact, and electromyogram-induced noise (EMG). BW noise is at a lower frequency, caused by respiration and motion of the subject or the leads. The frequency components of BW noise are usually below 0.5 Hz, and extend into the frequency range of the ST segment during a stress test. EMG noise, on the other hand, is predominantly at higher frequencies, caused by increased muscle activity and by mechanical forces acting on the electrodes. The frequency spectrum of the EMG noise overlaps that of the ECG signal and extends even higher in the frequency domain. In this article, the authors review some of the published ECG enhancing techniques to overcome the noise problems, and compare their performance on stress ECG signals under adverse noise scenarios. They also describe the filter bank-based ECG enhancing algorithm 相似文献
992.
H Korr V Philippi C Helg J Schiefer MB Graeber GW Kreutzberg 《Canadian Metallurgical Quarterly》1997,94(6):557-566
Unscheduled DNA synthesis (UDS) of nuclear DNA and mitochondrial (mt) DNA synthetic rates were determined autoradiographically in different cell types of the rodent brain 14 days after unilateral facial nerve transection. In addition to an increased synthetic rate of mtDNA in facial motoneurons 12 h after axotomy, a significant increase of UDS, i.e., DNA repair, and mtDNA synthesis were found in the regenerating facial nucleus 4 days after axotomy. Specificity of the observed labeling was confirmed by injection of 3H2O instead of [3H]thymidine. Using electron microscopic autoradiography, it was further shown that cytoplasmic labeling of neurons was mainly due to incorporation of radioactive label into mitochondria, indicating their subsequent multiplication by division. The observation that Northern blot signals for O6-alkylguanine-DNA-alkyltransferase mRNA from homogenized facial nuclei of both the axotomized and normal side remained unchanged over 14 days after axotomy indicated that the observed DNA-repair activity was not caused by endogenously produced alkylating agents. The combined presence of transiently increased UDS, enhanced mtDNA synthesis and elevated protein synthetic rates of regenerating motoneurons (as shown in the literature) suggests that free radicals produced by mitochondria in injured nerve cells could cause unspecific DNA damage followed by immediate repair. 相似文献
993.
IB Kremer CM Hilkens RM Sylva-Steenland CW Koomen ML Kapsenberg JD Bos MB Teunissen 《Canadian Metallurgical Quarterly》1996,157(5):1913-1918
The capacity of APC to stimulate the proliferation of human peripheral blood T cells decreases upon ultraviolet-B (UVB) irradiation. The aim of this study was to investigate whether all T cell subsets are equally sensitive to this reduced APC function. Established human Th1, Th2, and Th0 clones were stimulated with monocytes in a soluble CD3 mAb-mediated assay that is dependent on the presence of APC. Monocytes were exposed to low nonlethal doses of UVB radiation before coculture with T cells. UVB irradiation inhibited the capacity of monocytes to stimulate the proliferation and IFN-gamma production of Th1 cells in a dose-related fashion. In contrast, UVB-treated monocytes induced normal proliferation and IL-4 production in Th2 cells. Stimulation of Th0 cell proliferation by UVB-irradiated monocytes was normal, but a preferential suppression of IFN-gamma production was observed, thus leading to a more Th2-like cytokine response. The loss of Th1 proliferation upon stimulation with UVB-irradiated monocytes could be overcome by rIL-2; however, IFN-gamma production remained suppressed. IFN-gamma production could be completely restored by rIL-12, whereas the addition of IL-1 beta, TNF-alpha, or indomethacin had no such effect, nor did the addition of mAb to CD28, added to compensate for the reduced B7 expression of UVB-irradiated monocytes. Monocytes exposed to UVB radiation exhibited reduced expression of mRNA for the IL-1 2 subunits p35 and p40 and suppressed production of the IL-12 p70 protein. Our results thus indicate that UVB irradiation of APC selectively impairs Th1-like responses, a phenomenon caused by the UVB-induced suppression of monocyte IL-12 production. 相似文献
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T Surrey MB Elowitz PE Wolf F Yang F Nédélec K Shokat S Leibler 《Canadian Metallurgical Quarterly》1998,95(8):4293-4298
Chromophore-assisted light inactivation (CALI) offers the only method capable of modulating specific protein activities in localized regions and at particular times. Here, we generalize CALI so that it can be applied to a wider range of tasks. Specifically, we show that CALI can work with a genetically inserted epitope tag; we investigate the effectiveness of alternative dyes, especially fluorescein, comparing them with the standard CALI dye, malachite green; and we study the relative efficiencies of pulsed and continuous-wave illumination. We then use fluorescein-labeled hemagglutinin antibody fragments, together with relatively low-power continuous-wave illumination to examine the effectiveness of CALI targeted to kinesin. We show that CALI can destroy kinesin activity in at least two ways: it can either result in the apparent loss of motor activity, or it can cause irreversible attachment of the kinesin enzyme to its microtubule substrate. Finally, we apply this implementation of CALI to an in vitro system of motor proteins and microtubules that is capable of self-organized aster formation. In this system, CALI can effectively perturb local structure formation by blocking or reducing the degree of aster formation in chosen regions of the sample, without influencing structure formation elsewhere. 相似文献
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MB Pliss NIa Iatskovskaia MP Gulich GI Solomko VF Solov'eva 《Canadian Metallurgical Quarterly》1996,(10-12):107-111
A testing was done in a chronic experiment on 300 rats and 360 mice of both sexes for carcinogenic potential of a new protein product from Saccharomyces yeast grown in melasse. The production procedures and techniques of the above product have been worked out at the Ukrainian Research Institute of Spirits and Biotechnology of Food Stuffs of Gospishcheprom (State Food Industry) of Ukraine. The studies made showed the new protein product has no carcinogenic effect. 相似文献
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