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81.
H Sawert E Girardi G Antonucci MC Raviglione P Viale G Ippolito 《Canadian Metallurgical Quarterly》1998,158(19):2112-2121
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The objective of this study was to determine the disturbances of urea in serum and urine of endurance trained volunteers during prolonged exposure to hypokinesia (decreased number of km/day). The studies during hypokinesia (HK) were performed for 364 days on twenty long-distance runners in the age of 23 to 26 years, with an average peak oxygen uptake, of 66 ml.kg.min-1. All volunteers were divided into two equal groups: Ten volunteers were placed on a continuous regime of exercise 14.8 km/day and served as control subjects (CS). The remaining volunteers were subjected to continuous HK without the use of any preventive measures and were considered as the hypokinetic subjects (HS). For the simulation of the hypokinetic effect, the HS were kept continuously under an average of 2.7 km/day for the duration of the study. Prior to exposure to HK, the HS were on the same exercise regime as the CS. During a 60 day pre-HK period and during the experimental period, urinary excretion of urea, creatinine, sodium, potassium and calcium and concentrations thereof in serum were measured. In the HS the concentrations of urea, creatinine, sodium, potassium and calcium in serum and the rate of excretion thereof in urine increased significantly when compared to the CS. It was concluded that prolonged restriction of muscular activity induces significant disturbances of urea in serum and urine of endurance trained volunteers. 相似文献
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A Meneghetti WV Cardoso JS Brody MC Williams 《Canadian Metallurgical Quarterly》1996,44(10):1173-1182
Explants of embryonic lung are often used to characterize lung growth, bronchial tree pattern, and cell differentiation. Most investigators culture lungs for 3-7 days in defined media lacking, e.g., added growth factors or hormones. If growth and differentiation are comparable to that in vivo, these cultures show considerable promise for identifying developmental regulatory molecules and target genes, and for elucidating molecular responses. We used in situ hybridization and RT-PCR to compare times and sites of expression of mRNAs of six epithelial genes in cultured and uncultured fetal rat lungs. These genes, expressed in distal lung of adult rats, are surfactant proteins (SP) A, B, and C; LAR, a receptor-type tyrosine phosphatase; Clara cell secretory protein (CC10, CCSP); and T1alpha. SP-A, SF-B, LAR, and CC10 are expressed by both Clara and Type II cells in adult animals. SP-C and T1alpha are unique markers for Type II and Type I cells, respectively. SP-C, LAR, and T1alpha are expressed before the lung is explanted (Day 13.5); SP-A, -B, and CC10 mRNAs are first detected later. The onset of expression is similar in vivo and in vitro. Although the patterns of expression differ for each mRNA, their sites of expression in culture match those in vivo relative to the bronchial tree. The explanted embryonic lung appears to be an excellent experimental model. 相似文献
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Frédéric Gaschet 《Papers in Regional Science》2002,81(1):63-81
This article examines the relationships between the employment suburbanisation from central cities towards their suburbs,
and the process of intra-urban specialisation that occurred simultaneously in the fifty largest French metropolitan areas.
A methodology is proposed to identify urban subcentres and to analyse the effects of the intra-urban specialisations on suburbanisation
patterns. We conclude that the specialisation of both subcentres and central cities has a significant effect on suburbanisation
rates. Lastly, an intra-metropolitan shift/share analysis provides additional insights into the employment dynamics of central
cities and suburbs during the last twenty years.
Received: 25 July 2000 / Accepted: 29 May 2001 相似文献
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