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981.
982.
Dewsbury Donald A.; Bauer Stuart M.; Pierce John D.; Shapiro Lawrence E.; Taylor Stephen A. 《Canadian Metallurgical Quarterly》1992,106(4):383
Male prairie and montane voles (Microtus ochrogaster and M. montanus) were permitted 5 thrusts, without ejaculation, with a female at variable times after a 1st male ejaculated. In both prairie and montane voles, there were fewer sperm, in relation to control conditions, in the female's tract 1 hr after ejaculation if the female received thrusts immediately or 15 min after the ejaculate. There was no such effect after a 50-min delay. There was no significant decrease in litter production in prairie voles caused by thrusts delivered either immediately or after a 15-min delay. Sperm transport in these species is susceptible to disruption for a longer period than in deer mice or rats. The proposal that the postejaculatory interval (PEI) protects a male from disrupting its own sperm transport (the PEI matching law) appears not to hold for these species. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
983.
The hypothesis that psychosis-prone students demonstrate a pattern of exaggerated perceptual asymmetry across both left- and right-hemisphere dichotic-listening tasks (consonant-vowel [CV] and tonal contour discrimination) was investigated. Ss who scored high on the Perceptual Aberration or Magical Ideation scale or both (n?=?20) demonstrated a significantly exaggerated right-ear advantage on a CV task in relation to normal control Ss (n?=?27) but showed a reduced left-ear advantage on a tone task. The hypothesis of exaggerated functional lateralization across hemispheres in the psychosis-prone Ss was not supported, but the results are consistent with a hypothesis of left hemisphere overactivation in this sample. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
984.
Summary The problem of a laminar thin-film flow along a vertical wall is examined here. Using a fourth-degree polynomial velocity profile function inside the boundary layer, the momentum integral equation is solved analytically to determine the boundary layer thickness and the corresponding film-thickness. 相似文献
985.
While power quality is basically voltage quality, it is not strictly a voltage issue. Since the supply system has a finite, rather than an infinite, strength, currents outside the direct control of the utility can adversely affect power quality. These are harmonic load currents, lightning currents, and fault currents. How do we quantify voltage aberrations indicative of power-quality problems? One must employ an accurate voltage-measuring device, such as an oscilloscope, to identify which of the voltage aberrations, discussed in the article, is causing the problem. Equipment to mitigate the power quality problems is discussed. The acquisition of new equipment from the power quality perspective is also discussed and a checklist of procedures for design, installation and testing is given. 相似文献
986.
987.
Owing to its inherent antimicrobial effect and positive charge, the expression of human lysozyme in bovine milk could be beneficial by altering the overall microbial level and the functional and physical properties of the milk. We have used transgenic mice as model systems to evaluate the expression of human lysozyme containing fusion gene constructs in the mammary gland. Expression of human lysozyme was targeted to the mammary gland by using the 5' promoter elements of either the bovine beta (line B mice) or alpha s1 (line H mice) casein genes coupled to the cDNA for human lysozyme. Expression of human lysozyme mRNA was not found in mammary tissue from any of line B mice. Tissues were analysed from six lines of H mice and two, H6 and H5, were found to express human lysozyme mRNA in the mammary gland at 42% and 116%, respectively, of the levels of the endogenous mouse whey acidic protein gene. At peak lactation, female mice homozygous for the H5 and H6 transgene have approximately twice the amount of mRNA encoding human lysozyme as hemizygous animals. Expression levels of human lysozyme mRNA in the mammary gland at time points representing late pregnancy, early, peak and late lactation corresponded to the profile of casein gene expression. Human lysozyme mRNA expression was not observed in transgenic males, virgin females or in the kidney, liver, spleen or brain of lactating females. A very low level of expression of human lysozyme mRNA was observed in the salivary gland of line H5. 相似文献
988.
Microbial phosphatases are known or suspected to play a role in the pathogenesis of several intracellular pathogens, including Legionella micdadei. Legionella pneumophila also possess phosphatase activities, but their possible roles in cellular infection are unknown. We generated mutants of a serogroup 1 isolate of L. pneumophila that lack the major phosphatase. Isolation of a Pho- mutant after random mutagenesis with transposon MudII4041 allowed us to dissociate the major alkaline phosphatase (pH optimum approximately 8) from a minor acid phosphatase activity. Both activities were concentrated in the bacterial periplasm. The gene encoding the major alkaline phosphatase (pho) was cloned by expression in E. coli and used to generate a site directed mutation in two L. pneumophila strains. Each parent-mutant pair was compared in a U937 cell tissue culture assay for capacity to infect, lyse, and grow within mammalian cells. Although the parental stains differed in their U937 cell cytopathicity, neither was significantly more infective than its Pho- derivative, suggesting that the alkaline phosphatase activity is not essential for cellular infection. Because they are not attenuated, Pho- mutants can be used to generate gene fusions with E. coli alkaline phosphatase to study and secretion and cellular infectivity in L. pneumophila. 相似文献
989.
990.
JJ Goronzy P Bartz-Bazzanella W Hu MC Jendro DR Walser-Kuntz CM Weyand 《Canadian Metallurgical Quarterly》1994,94(5):2068-2076
Clonal expansion of T cell specificities in the synovial fluid of patients has been taken as evidence for a local stimulation of T cells. By studying the T cell receptor (TCR) repertoire of CD4+ T cells in the synovial and peripheral blood compartments of patients with early rheumatoid arthritis (RA), we have identified clonally expanded CD4+ populations. Expanded clonotypes were present in the peripheral blood and the synovial fluid but were not preferentially accumulated in the joint. Dominant single clonotypes could not be isolated from CD4+ cells of HLA-DRB1*04+ normal individuals. Clonal expansion involved several distinct clonotypes with a preference for V beta 3+, V beta 14+, and V beta 17+CD4+ T cells. A fraction of clonally related T cells expressed IL-2 receptors, indicating recent activation. The frequencies of clonally expanded V beta 17+CD4+ T cells fluctuated widely over a period of one year. Independent variations in the frequencies of two distinct clonotypes in the same patient indicated that different mechanisms, and not stimulation by a single arthritogenic antigen, were involved in clonal proliferation. These data support the concept that RA patients have a grossly imbalanced TCR repertoire. Clonal expansion may result from intrinsic defects in T cell generation and regulation. The dominance of expanded clonotypes in the periphery emphasizes the systemic nature of RA and suggests that T cell proliferation occurs outside of the joint. 相似文献