Comparison of the measurements of total IGE using the CAP-RIA technique and the UNICAP-FEIA technique

The contribution of a new method of measurement of total IgE by the Fluoro-Enzymo-Immuno-assay (FEIA) with the assistance of Pharmacia UNICAP automation is very reliable since, when compared with the measurement by the CAP SYSTEM RIA on 144 patients who were clinically allergic (84 with a raised and 60 with a normal IgE titre) the result of a comparative study between the two techniques is statistically highly significant. The two methods were also compared by age groups and these results were also statistically highly significant.     

Primary structure of the kinase domain region of rabbit skeletal and cardiac muscle titin

A 2.3 kb region of rabbit cardiac and skeletal muscle titin has been cloned. The cDNA sequences of the two tissues are identical and show 91% identity on the nucleotide level with the corresponding region of human cardiac muscle titin. On the amino acid level the identity is 96% and similarity is 98%. Alignment of predicted amino acid sequences of several homologous kinase domains reveals that the rabbit titin kinase has all the necessary elements of an active catalytic domain and carries a potential regulatory region on its C-terminal end. The distance of the 2.3 kb contig from the 3' end of the message was determined to be 5.7 kb in both tissues using oligonucleotide directed RNase H cleavage of titin mRNAs. This is essentially identical with the length of the fully sequenced human cardiac titin C-terminal end. It therefore appears unlikely that there are major tissue specific differences in this 8 kb cDNA region which encodes the C-terminus of rabbit skeletal and cardiac titin.     

The effects of semen dosage and insemination frequency on the fertility of brolier breeder hens

Sexuality mature commerical broiler breeder pullets were housed singly in laying cages and artifically inseminated with one of three different levels of undiluted pooled semen (0.1, 0.05 and 0.025 ml.) at either of two different frequencies (once and twice a week) in three different trials. The best fertility was obtained by inseminating twice a week with 0.05 ml. of semen. Inseminating once a week with 0.1 ml. of semen did not provide maximum fertility in the stock used in this study.     

Second-harmonic locking properties of l.s.a.-mode transferred-electron oscillators

Harmonic injection locking of an l.s.a.-mode oscillator has been studied by computer simulation. The results show that under certain circuit conditions the 2nd-harmonic frequency locking range may exceed that of the fundamemtal.     

Kinetics and catalysis of the reaction of coal char and steam

The kinetics and catalysis of the reaction between steam and two different coal chars were investigated in a small fixed-bed reactor. Atmospheric pressure and temperatures between 600 and 850 °C were used. Various possible catalysts were tested, and the effect of catalyst concentration was examined. Of the catalysts used, potassium carbonate was the most effective. It was followed in order of decreasing effectiveness by sodium carbonate, lithium carbonate, potassium chloride, sodium chloride, and copper (II) oxide. Calcium oxide and iron (III) oxide were totally ineffective. The catalysts were more effective at higher concentration. Also, addition of 10% potassium carbonate to the coal char lowered the apparent activation energy of the reaction from 254.2 to 144.5 kJ/mol and the frequency factor from 2.41 × 10⁸ to 1.32 × 10⁴ s^?1. The water-gas shift reaction reached equilibrium during gasification in the presence of alkali-metal carbonates and the product gas was primarily carbon dioxide and hydrogen. Without catalyst present, the water-gas shift reaction was far from equilibrium.