A feasibility study of Lactobacillus plantarum in fruit powders after processing and storage

The aim of this study was to develop fruit powders (apple, banana and strawberry) enriched with a probiotic strain (Lactobacillus plantarum 299v). Two methodologies were proposed: (i) drying of the fruit with probiotic culture incorporated (by convection) or (ii) drying of fruit (by convection) and addition of spray‐dried probiotic culture. In the first methodology, processing caused a notable reduction in probiotic viable counts in apple, but this reduction was lower during drying of banana and strawberry. A large reduction in viable cells was also recorded during storage. In the second methodology, the survival of L. plantarum 299v was considerably higher during spray‐drying, and fruit powders with a microbial content suitable for a probiotic food (10⁸–10⁹ cfu g^?1) were obtained. The fruit powders incorporating L. plantarum 299v can be stored at 4 °C or at room temperature, for at least 3 months. This preliminary study demonstrated that fruit powders are good carriers of probiotic cultures, but the techniques used to produce them should be carefully considered.     

Socially-Aware Multimedia Dissemination in Personal Mobile Networks

Wireless Personal Communications - Mobile multimedia entertainment is pervasive on mobile devices, leading people to access, store and disseminate a diversity of multimedia contents anytime and...     

High surface area LaNiO<Subscript>3</Subscript> electrodes for oxygen electrocatalysis in alkaline media

LaNiO₃ coatings on nickel-foam supports were prepared by brush painting. The electrochemical properties were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Comparative studies were performed with LaNiO₃-pelleted electrodes. The roughness factors were determined by CV and found to be 5,208 ± 350 and 4,037 ± 250 for the pelleted and coated electrodes, respectively. EIS measurements confirm the results obtained by CV. Values lower than 0.3 were calculated for the morphology factors for both electrodes, indicating low electrochemical porosity. The experimental method used in this work to synthesise the oxide coupled with the use of Ni foam as support has proved to be very effective in producing oxide electrodes with surface areas higher than those referred to in relevant literature.     

Scenario evaluation for the management of household solid waste in small Brazilian municipalities

As achieving Brazil’s National Policy on Solid Waste-based goals effectively is not simple, there are alternatives such as intermunicipal cooperation by consortium or privatization. Therefore, this study aimed to evaluate the Greenhouse Gas emissions and energy use of six scenarios in two landfills contexts (private and consortium) for small municipalities (less than 100,000 inhabitants as per Brazilian standards) located in São Paulo, Brazil. Through the technique of scenario building and method of Waste Reduction Model was possible to obtain the total energy, the amount of carbon dioxide equivalent (CO₂E) and carbon equivalent (CE) emissions. The best scenario integrates composting and recycling, reducing 72 % for CO₂E and CE emissions, and saving about 410 % in energy. The landfill consortium will only take advantage, if the location is the most ideal as far as possible. Small municipalities that do not dispose their waste in landfills compatible with regulatory standards may seek for intermunicipal cooperation and adopt integrated waste-management programmes to reduce their environmental impacts.     

Effect of Vertical Misfit on Screw Joint Stability of Implant-Supported Crowns

The passive fit between prosthesis and implant is a relevant factor for screw joint stability and treatment success. The aim of this study was to evaluate the influence of vertical misfit in abutment-implant interface on preload maintenance of retention screw of implant-supported crowns. The crowns were fabricated with different abutments and veneering materials and divided into 5 groups (n = 12): Gold UCLA abutments cast in gold alloy veneered with ceramic (Group I) and resin (Group II), UCLA abutments cast in titanium veneered with ceramic (Group III) and resin (Group IV), and zirconia abutments with ceramic veneering (Group V). The crowns were attached to implants by gold retention screws with 35-N cm insertion torque. Specimens were submitted to mechanical cycling up to 10⁶ cycles. Measurements of detorque and vertical misfit in abutment-implant interface were performed before and after mechanical cycling. ANOVA revealed statistically significant difference (P < 0.05) among groups for vertical misfit measured before and after mechanical cycling. The abutments cast in titanium exhibited the highest misfit values. Pearson correlation test did not demonstrate significant correlation (P > 0.05) between vertical misfit and detorque value. It was concluded that vertical misfit did not influence torque maintenance and the abutments cast in titanium exhibited the highest misfit values.     

District Metered Areas Design Under Different Decision Makers’ Options: Cost Analysis

Water loss is a big challenge for water supply companies worldwide, and the Water Network Partitioning (WNP) is an excellent tool for water loss management–particularly in the current difficult economic and financial conditions. WNP is a recent research line and consists in dividing the water distribution network into smaller zones called District Metered Areas (DMAs) with one (or more, in exceptional cases) supply point, to reduce the network complexity and/or allow pressure management. Since there are several possible future scenarios, such as the water demand and/or the infrastructure degradation forecasts, which may have different impacts on the hydraulic behaviour, in this paper a computational application, based on an optimization model, is proposed to achieve a compromise between robustness required for the DMAs design (using a baseline scenario) and different decision makers’ options (using other scenarios with a lower “probability” of occurrence), to reduce the total cost. The objective function reflects the minimization of the squared deviations between the total cost of the DMAs design and the minimum cost for each scenario forecasted for the project plan, multiplied by the weight or “probability” of occurrence for each of the scenarios. The performance of the computational application is illustrated with a case study, and the results are encouraging.     

The Difficulties of Designing Future Coastlines in the Face of Climate Change

This paper adopts two perspectives. The first is a framing process aimed at defining and examining the conditions for adopting adaptive coastal governance. The second applies to relevant themes of changing coastal policy, central to the testing of adaptive coastal governance, namely cooperative science, risk-sensitive planning, socially fair insurance cover and effective ways to design, finance and engage with local communities over actual coastal change. We illuminate both missions through case studies in North Norfolk (England) and Portugal, all notably affected by coastal change. In England and Portugal, there is a broad understanding and acceptance of the likely effects of climate change. This recognition encourages debates over risk-averse planning, the design of proactive insurance cover, creative relocation of endangered property and new ways of predicting and paying for coastal adjustment. Yet, moving from a basic willingness to engage with coastal change to actual practices of landscape adjustment through such policy shifts is proving very difficult. In this research, we find that coastal landscapes are lived experiences, resigned acceptances of inevitable change and hopeful imaginings. Coastal management institutions are not geared to resolving this incompatibility and this paper explains why.     

Effect of self-etching dual-curing universal adhesive system application on bond strength of dentin resinous liners dentin

Purpose: The aim of this study was to evaluate the influence of previous application of an adhesive system on bond strength of resinous liner materials to dentin. Methods: Ninety bovine incisors crowns had a 6 × 6 mm area of dentin exposed, with minimum of 2 mm thickness. They were embedded in acrylic resin, and the dentin was polished with P600 SiC sandpaper for 30 s to standardize the smear layer. The specimens were divided into 6 groups (n = 15) according to the application or not of a self-etching system (Futurabond U – Voco) and the type of resinous liner used: A+Ionoseal (adhesive and Ionoseal – Voco); Ionoseal (Ionoseal only); A+Vitrebond (adhesive and Vitrebond – 3M/ESPE); Vitrebond (Vitrebond only); A+Ionosit (adhesive and Ionosit – DMG) and Ionosit (Ionosit only). Adhesives were used following manufacturer’s instructions, and the liner materials were applied inside a 2-mm-depth matrix and light-cured for 20 s. The bond strength was measured by microtensile test, using a universal testing machine with a cross-speed of 1 mm/min. Data were analyzed using one-way ANOVA and Tukey’s test (p < 0.05). Results: The adhesive system application increased bond strength of all liners tested. Ionoseal presented the highest bond strength when the adhesive system was used and exhibited similar performance to Vitrebond without adhesive. Ionosit without adhesive showed the smallest bond strength compared with the other liners tested. Conclusion: The application of an adhesive system prior to the use of the resinous liners improved the bond strength to dentin and should be preconized.     

A Quinacrine Analogue Selective Against Gastric Cancer Cells: Insight from Biochemical and Biophysical Studies

One of the earliest synthetic antimalarial drugs, quinacrine, was recently reported as interesting for the treatment of acute myeloid leukemia. Inspired by this and similar findings, we evaluated a set of quinacrine analogues against gastric (MKN‐28), colon (Caco‐2), and breast (MFC‐7) cancer cell lines and one normal human fibroblast cell line (HFF‐1). All the compounds, previously developed by us as dual‐stage antimalarial leads, displayed antiproliferative activity, and one of the set stood out as selective toward the gastric cancer cell line, MKN‐28. Interestingly, this compound was transported across an in vitro MKN‐28 model cell line in low amounts, and approximately 80 % was trapped inside those cells. Nuclear targeting of the same compound and its interactions with calf thymus DNA were assessed through combined fluorescence microscopy, spectroscopy, and calorimetry studies, which provided evidence for the compound's ability to reach the nucleus and to interact with DNA.