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This study measured volumetric liver blood flow and galactose clearance concurrently during orthotopic liver transplant in human subjects. Ultrasound transit time flowmeters measured hepatic artery and portal vein flow 1-3 h after reperfusion. Galactose (100 mg/min) was infused over 45-60 min to steady state for calculation of clearance. Mean +/- S.D. total volumetric flow was 1966 +/- 831 ml/min with portal flow contributing 86%. Mean galactose clearance was 1988 +/- 641 ml/min. There was a significant correlation (p < 0.05, r = 0.61) between volumetric total liver blood flow and galactose clearance. The data show that: (i) the newly transplanted liver is capable of metabolizing galactose within 1-3 h of reperfusion; and (ii) liver blood flow is high in the newly implanted liver. The clinical importance of this observation is that there is increased clearance of high first pass substances by the transplanted liver which may be of importance in patient management. 相似文献
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V Robert F De Giorgi ML Massimino M Cantini T Pozzan 《Canadian Metallurgical Quarterly》1998,273(46):30372-30378
Direct monitoring of the free Ca2+ concentration in the sarcoplasmic reticulum (SR) was carried out in rat skeletal myotubes transfected with a specifically targeted aequorin chimera (srAEQ). Myotubes were also transfected with a chimeric aequorin (erAEQ) that we have demonstrated previously is retained in the endoplasmic reticulum (ER). Immunolocalization analysis showed that although both recombinant proteins are distributed in an endomembrane network identifiable with immature SR, the erAEQ protein was retained also in the perinuclear membrane. The difficulty of measuring [Ca2+] in 100-1000 microM range was overcome with the use of the synthetic coelenterazine analogue, coelenterazine n. We demonstrate that the steady state levels of [Ca2+] measured with srAEQ is around 300 microM, whereas that measured with erAEQ is significantly lower, i.e. around 200 microM. The effects of caffeine, high KCl, and nicotinic receptor stimulation, in the presence or absence of external calcium or after blockade of the Ca-ATPase, were investigated with both chimeras. The kinetics of [Ca2+] changes revealed by the erAEQ were similar, but not identical, neither quantitatively nor qualitatively, to those monitored with the srAEQ, indicating that at this stage of muscle development, differences exist between SR and ER in their mechanisms of Ca2+ handling. The functional implications of these findings are discussed. 相似文献
37.
RM Martín‐Aranda E Ortega‐Cantero ML Rojas‐Cervantes MA Vicente‐Rodríguez MA Baares‐Muoz 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2005,80(2):234-238
α,β‐Unsaturated nitriles have been synthesized by Knoevenagel condensation of a carbonylic compound with malononitrile, assisted by sonochemical irradiation. Two alkaline‐promoted clays (Li+‐ and Cs+‐exchanged saponites) have been employed as catalysts. The influence of the carbonylic compound (benzaldehyde or cyclohexanone) and the use of a solvent on the catalytic activity have been studied. Remarkable increase in the conversion values has been found when the reaction is activated by ultrasound, as compared with the thermal activation. In this green, solvent‐free procedure, α,β‐unsaturated nitriles have been produced in very high yields (97%) when the Cs+‐saponite is used as catalyst. Copyright © 2004 Society of Chemical Industry 相似文献
38.
Hydrogenation of canola oil in the presence of nickel and the methyl benzoate-chrome carbonyl complex 总被引:1,自引:0,他引:1
L. J. Rubin S. S. Köseoglu L. L. Diosady W. F. Graydon 《Journal of the American Oil Chemists' Society》1986,63(12):1551-1557
Canola oil was hydrogenated using a mixture of homogeneous methyl benzoate-Cr(CO)3 and heterogeneous nickel catalysts. The effect of the methyl benzoate-Cr(CO)3_to-nickel ratio on the activity, specific isomerization index, linoleate and linolenate selectivities, and fatty acid composition
was evaluated, and the results compared with those obtained with commercial nickel catalyst and methyl benzoate-Cr(CO)3 used individually. At higher chromium-to-nickel ratios the activity of nickel was inhibited and the system behaved essentially
like the pure chrome complex, while at low chromium-to-nickel ratios the characteristics of the nickel predominated. In a
short transition zone relatively high reaction rates were obtained with significantly reducedtrans-isomer levels in the product. In a broader sense, it may be possible to combine a homogeneous and heterogeneous catalyst
while retaining the advantages of both. We may thus be able to design catalyst systems for specific applications. 相似文献
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Platelet aggregation is known to be inhibited by ethanol, and this has been suggested to be one of the attenuating effects
of ethanol in cardiovascular disease. Recent studies have implicated an inhibition of phospholipase A2 induced arachidonic acid release, since the production of prostanoids that are formed from arachidonic acid and are involved
in the aggregation process has been shown to be diminished by ethanol. Phospholipase A2 is found in platelets in both a cytosolic form, from where it may translocate to the plasma membrane to release arachidonic
acid, and in a secretory form which is released extracellularly upon activition. In the present study, the effect of ethanol
on the secretion of phospholipase A2 and on its activity was determined. It was found that ethanol inhibited trast, the activity of the cytosolic form of phospholipase
A2 was inhibited by ethanol. 相似文献