Cholinergic blockade, septal lesions, and DRL performance in the rat.

Reports 4 experiments with a total of 20 male Long-Evans hooded rats describing the effects of cholinergic blockade produced by systemic injection of either atropine sulfate or atropine methyl nitrate on Ss' differential reinforcement of low rate (DRL) responding. It was shown that atropine sulfate injected either chronically or at high dosages suppressed DRL responding. Injected acutely, atropine sulfate produced disinhibitory effects. When atropine was injected either chronically or acutely into Ss with septal lesions, there was suppression of responding. It is suggested that the specific behavioral outcome resulting from cholinergic blockade depends on the balance resulting from the competing peripheral and central effects of such blockade. (27 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Pressure Effect on Space-Charge-Limited Current Transmitted across a Cylindrical Cavity

Experimental data on space-charge dissipation due to gas effects has been obtained using electron pulses from an accelerator injected directly through a thin film and a metal screen into a small experiment chamber. The current transmitted across the chamber was measured as a function of gas pressure, gas composition, chamber depth, and electron collector impedance. The measurements generally confirm the theory developed by dePlomb in a companion paper at this conference. Several problem areas are noted that require further investigation.     

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Etudes magnetiques et cristallographiques depuis 4, 2K de la solution solide (Ga1−xZnx)Mn3C

The magnetic and crystallographic measurements were carried on the (Ga_1?xZn_x)Mn₃C solid solutions as a function of temperature from 4, 2K to 320K. The first order and second order magnetic transformations have been observed as a function of the composition in this metallic perovskites. The phase diagram shows four different ordered magnetic structures.     

Chromosomal anomalies in stage D1 prostate adenocarcinoma primary tumors and lymph node metastases detected by fluorescence in situ hybridization

We previously reported a HPLC assay method using fluorimetric detection for the simultaneous determination of urinary N2-(3-aminopropyl)biopterin (oncopterin, a natural pteridine newly found in urine from cancer patients), biopterin and neopterin. We now have observed that an unknown substance, which may be derived from methotrexate, in urine from a patient with stomach cancer interfered with the assay of oncopterin and demonstrated that oncopterin could be completely separated from the unidentified substance by HPLC using a Nucleosil 100-5SA strong cation-exchange column. Furthermore, oncopterin was not detectable by this HPLC-fluorimetric method in urine samples from patients with stomach cancer who were not treated with methotrexate. The content of urinary oncopterin from cancer patients is supposed to be very low, with less than 1 mumol/mol creatinine. The present results indicate that the peak found with elution from the C18 column was a methotrexate-derived compound and co-eluted with the analyte oncopterin.     

Cholesterol-independent targeting of Golgi membrane proteins in insect cells

Distinct lipid compositions of intracellular organelles could provide a physical basis for targeting of membrane proteins, particularly where transmembrane domains have been shown to play a role. We tested the possibility that cholesterol is required for targeting of membrane proteins to the Golgi complex. We used insect cells for our studies because they are cholesterol auxotrophs and can be depleted of cholesterol by growth in delipidated serum. We found that two well-characterized mammalian Golgi proteins were targeted to the Golgi region of Aedes albopictus cells, both in the presence and absence of cellular cholesterol. Our results imply that a cholesterol gradient through the secretory pathway is not required for membrane protein targeting to the Golgi complex, at least in insect cells.