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根据企业特点 ,在贯彻ISO90 0 2标准中 ,建立健全设备管理机构及制度体系 ,提高企业设备管理水平 ,建立设备台帐等提出了几点看法 相似文献
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We examined the effects of different cytokine combinations and culture conditions on the expansion and modulation of cell surface antigens of CD34+ derived dendritic cells (DCs), the most efficient antigen-presenting cells capable of stimulating resting T cells in the primary immune response. Cells with a dendritic morphology and expressing HLA-DR, CD1a, S100 and CD83 were maximally expanded under serum-free conditions with the addition of SCF, GM-CSF, TNF-alpha, TGF-beta and Flt-3 ligand (fold increase of CD1a+ cells = 102 +/- 32 after 2 weeks of culture). CD34+ cells were also grown under continuous flow conditions in an artificial capillary system: after 14d of culture, the expansion in the total cell number was lower than that of the static cultures (3.3 +/- 2 v 18.9 +/- 4) but the percentage of CD1a+/CD83+/ CD80+ cells was considerably higher, whereas the CD14+ cells were significantly reduced (8.9 +/- 2 v 26 +/- 13). In continuous perfusion cultures, low levels of DC precursors and of LTC-IC were still present up to day 14. The DCs generated under flow conditions stimulated the mixed leucocyte reaction (MLR) more than the cells grown in static cultures. By electron microscopy, cells grown in the continuous flow system showed an increased number of large cells with numerous dendritic processes and abundant multilamellar complexes. The cells expanded under these conditions were sorted on the basis of their light-scatter properties into two fractions: one containing a predominance of CD1a+/S100+/ CD8 3+/CD80+/CD14- 'large cells' with great internal complexity (mature DCs); the second including 'small cells' either CD33+/CD14+, CD33+/CD15+ or CD33+/CD13-/CD14. The DCs generated and selected with this method are therefore particularly well suited for immunotherapeutic protocols. 相似文献
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Effect of routine screening for Down's syndrome on the significance of isolated fetal hydronephrosis
OBJECTIVE: To determine the risk of Down's syndrome in fetuses with isolated hydronephrosis at 18-23 weeks in an unselected general population after routine screening for Down's syndrome, using first trimester nuchal translucency measurement and second trimester maternal serum biochemistry. POPULATION: All pregnant women undergoing a routine 18-23 week ultrasound scan, from a population who had been offered screening for Down's syndrome. SETTING: A district general hospital serving a low risk obstetric population. METHODS: Prospective study of all routine 18-23 weeks ultrasound scans. The prevalence of isolated hydronephrosis and Down's syndrome was determined and the relative risk for Down's syndrome was calculated for different ultrasound findings. RESULTS: 10,971 women were scanned at 18-23 weeks during the study period. Down's syndrome was diagnosed in 14 of 20 cases before this stage using first trimester nuchal translucency measurement and second trimester maternal serum biochemistry. Isolated fetal hydronephrosis was diagnosed in 423 pregnancies (3.9%); none of these pregnancies were affected by Down's syndrome. The relative risk for Down's syndrome was 0.18 (95% CI 0.06-0.53) for women with a normal scan (n = 9983). When multiple ultrasound markers were found (n = 565), the relative risk for Down's syndrome was 2.00 (95% CI 0.18-22.10) and 9.00 (95% CI 1.14-71.30) for all other aneuploidies. CONCLUSION: The finding of isolated fetal hydronephrosis does not significantly increase the age-related risk for Down's syndrome. The presence of multiple ultrasound markers is associated with an increased risk of aneuploidies other than Down's syndiome. These findings are explained by the reduced prevalence of Down's syndrome as a result of prior screening and diagnosis of this condition. 相似文献
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一种基于几何分布的新支持向量机多分类方法 总被引:1,自引:0,他引:1
二叉树支持向量机是多分类问题的一种有效方法,然而分类的效果与二叉树的结构密切相关。获得更好的分类效果和更高的效率,要使得二叉树高度尽量小而两个子类尽量易分。距离通常用来衡量两个类的分离程度,但不能反映类的分布情况。考虑到多分类中类的分布,文中定义新的分离度和相似度来衡量两个类的分离度,并且提出了一中新的基于几何分布二叉树支持向量机多分类算法,该方法使得二叉树高度尽量小而两个子类尽量易分。实验表明该方法具有较高的分类准确率和效率。 相似文献
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计算机公共课作为非计算机专业本科学生的公共基础课程核心之一,在教学组织、教学内容、教学方法、实践环节等方面还存在很多亟待解决的问题。针对目前存在的问题,结合相关的指导性文件,探讨解决的思路及对策。 相似文献
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针对掏挖与岩石锚杆复合型基础上拔承载机理不明确的问题,基于现场试验工况,利用Plaxis3D有限元软件建立复合型基础抗拔承载模型,研究其上拔承载机理。在此基础上,研究了土体弹性模量、岩体弹性模量、土体黏聚力对复合型基础各部分的承载影响,分析了掏挖基础和岩石群锚基础的承载力发挥情况、上拔变形、塑性区开展,并给出了复合型基础的改进方案。结果表明:上拔变形刚度较大的岩锚基础对复合型基础的承载极限状态起控制作用;掏挖基础和岩石锚杆基础的上拔变形协调性是影响复合型基础承载力发挥的关键;适当增加掏挖基础嵌入岩石的深度可有效提高复合型基础的抗拔承载力。 相似文献
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