Realization of electroplating molds with thick positive SPR 220-7 photoresist

This paper reports on progress on the feasibility of fabricating molds for electroplating of medium-to-high aspect ratio structures (up to 5) using SPR 220-7 positive photoresist, commercialized by Shipley [1, 2]. We used this photoresist as an alternative to SU-8 negative epoxy-based photoresist, which is very difficult to process and remove after electroplating [3, 4]. SPR 220-7 is easy to work with and can be used as a sacrificial layer if removed after processing. A single layer of SPR 220-7 was deposited by spin coating up to 18 m thickness. Thicker layers (34–54 m) can be achieved with multi-layer coating/baking steps using a manual spin coater and oven. The motivation of this work is to realize micromachined high-density current-carrying wires for atom guides [5, 6]. However, the process can be used for a variety of applications such as on-chip inductors and microtransformers.     

Risk stratifying patients who survive an acute myocardial infarction

While androgens have important skeletal effects, the mechanism(s) of androgen action on bone remain unclear. Current osteoblast models to study androgen effects have several limitations, including the presence of heterogeneous cell populations. In this study, we examined the effects of androgens on the proliferation and differentiation of a novel human fetal osteoblastic cell line (hFOB/AR-6) that expresses a mature osteoblast phenotype and a physiological number (approximately 4,000/nucleus) of androgen receptors (AR). Treatment with 5alpha-dihydrotestosterone (5alpha-DHT) inhibited the proliferation of hFOB/AR-6 cells in a dose-dependent fashion, while it had no effect on the proliferation of hFOB cells, which express low levels of AR (<200/nucleus). In hFOB/AR-6 cells, co-treatment with the specific AR antagonist, hydroxyflutamide abolished 5alpha-DHT-induced growth inhibition. Steady-state levels of transforming growth factor-beta1 (TGF-beta1) and TGF-beta-induced early gene (TIEG) mRNA decreased after treatment of hFOB/AR-6 cells with 5alpha-DHT, suggesting a role for the TGF-beta1-TIEG pathway in mediating 5alpha-DHT-induced growth inhibition of hFOB/AR-6 cells. In support of this, co-treatment of hFOB/AR-6 cells with TGF-beta1 (40 pg/ml) reversed the 5alpha-DHT-induced growth inhibition, whereas TGF-beta1 alone at this dose had no effect on hFOB/AR-6 cell proliferation. Furthermore, treatment of hFOB/AR-6 cells with 5alpha-DHT and testosterone (10(-8) M) inhibited basal and 1,25-(OH)2D3-induced alkaline phosphatase (ALP) activity and type I collagen synthesis without affecting osteocalcin production. Thus, in this fetal osteoblast cell line expressing a physiological number of AR, androgens decrease proliferation and the expression of markers associated with osteoblast differentiation. These studies suggest that the potential anabolic effect of androgens on bone may not be mediated at the level of the mature osteoblast.     

Amino acid and energy interrelationships in growing beef steers: II. Effects of energy intake and metabolizable lysine supply on growth

We conducted three experiments to determine the optimal metabolizable Lys:net energy ratio for growth of beef calves. The single basal diet fed contained corn (56.1%), soybean hulls (18%), cottonseed hulls (15%), animal fat (4.25%), and corn gluten meal (5.6%). In Exp. 1, 54 steers were individually fed the basal diet at 1.5, 2.25, and 3.0 times NEm requirement; rations were top-dressed with 3.4 g of rumen-stable (RS) Met and either 0, 2, 4, 6, 8, or 12 g of RS-Lys daily. An additional 18 steers were fed the same three levels of energy and supplemented with 125 g of blood meal per steer. In Exp. 2, 68 crossbred steers were subjected to the same experimental protocol, with the exception that only the two highest levels of energy were used. Of these steers, 48 were fed individually and received the RS-Lys treatments; the remaining 20 steers received 125 g of blood meal per steer. No interaction (P > .10) was detected between level of supplemental Lys and energy intake in Exp. 1 or 2. Supplementation with RS-Lys improved (P < .01) ADG in Exp. 1, but it had no effect (P > .10) on growth in Exp. 2. The Lys requirement estimates were 44.3 and 51.3 g/d, corresponding to maximal growth rates of 1.21 and 1.64 kg/d for the 2.25 and 3.0 times maintenance treatments, respectively. Comparing the growth rates of steers fed supplemental Lys with those of steers fed blood meal in Exp. 1 and 2 revealed an ADG advantage (P < .03) with blood meal supplementation. To confirm the blood meal response, Exp. 3 used 75 crossbred steers fed the basal diet at 3.0 times NEm requirement plus either 3.4 g RS-Met, 3.4 g RS-Met and 12 g RS-Lys, or 125 g of blood meal per steer. Blood meal supplementation improved (P < .01) growth of steers over those fed supplemental Met or Met plus Lys. Although a distinct relationship between amino acid requirements and energy supply may exist, Lys and Met were not first-limiting in these experiments, or selective supplementation with undegradable protein may have provided some factor that enhanced performance beyond that detected with Lys and Met alone.     

A novel method for DEAE-dextran mediated transfection of adherent primary cultured human macrophages

The effects of regional and global ischemia on cellular electrical activity and on arrhythmias induced by reperfusion were studied at different Mg2+ concentrations (Mg2+o, 0, 1.2, and 4.8 mM) in perfused rat hearts. Surface electrograms and transmembrane potentials were recorded during control, 10 min of ischemia (perfusion arrest or coronary ligation), and reperfusion. Increasing Mg2+o from 0-4.8 mM decreased heart rate, did not alter action potential morphology, and had a strong antiarrhythmic action on reperfusion following coronary ligation. At low and normal Mg2+o, the incidence of tachyarrhythmias was between 70 and 80%. Global ischemia led to progressive atrioventricular block and the final ventricular beating rate was similar at all Mg2+o despite unequal initial values. The severity of arrhythmias was similar to that found after regional ischemia in Mg2+o = 0, but much lower at normal and high Mg2+o. The resting depolarization induced by coronary ligation decreased as Mg2+o was raised, but such a relation was not seen during global ischemia where the depolarization was less marked. The action potential duration did not vary with the ventricular rate between 160 and 380 beats per min but increased considerably when sinus rate was markedly slowed (40 to 80 bpm) by raising Mg2+o to 9.6 mM. Our data show that a high Mg2+o exerts a strong protection against reperfusion arrhythmias regardless of the type of ischemia. Modulation of the sinus rhythm by Mg2+ may contribute to its protective effect by decreasing K+o accumulation and Na+i loading during ischemia.     

Incorporating experimental uncertainties into multivariate granulation modelling

A methodology that carries experimental uncertainties into model predictions is studied and applied to a multidimensional population balance model for granulation processes. This complex model contains 27 parameters. A portion of them such as material constants can be measured or estimated, whereas some of the model parameters need to be established through granulation experiments and subsequent fitting to the model. As uncertainties are associated with every measurement, these are used in the presented methodology for the computation of uncertainties in the model predictions. This allows one to assess the quality of a model and to identify outliers in the experimental observations. As the evaluation of the complex model framework is computationally expensive, the granulation process is approximated with response surfaces in the studied example, allowing the quick computation of the model response in the optimisation procedure. Using eight sets of experimental observations, model-specific rate constants for particle coalescence, compaction, breakage, and reaction are calculated. Additionally, uncertainties of these parameters are estimated, allowing for the calculation of the model prediction and its uncertainty. Whereas the a priori uncertainties are relatively large, the uncertainties are significantly reduced by the method proposed. In addition to this, a possible mismatch between the model and the experimental observations is identified, giving hints for further investigations.     

Antithrombotic potency of hirudin is increased in nonhuman primates by fibrin targeting

BACKGROUND: Inhibition of thrombin by either the indirect thrombin inhibitor heparin or by more potent direct thrombin inhibitors such as hirudin reduces thrombus formation after arterial injury. The present study was designed to determine if a fibrin-specific thrombin inhibitor could, by local thrombin inhibition, prevent thrombosis more effectively. METHODS AND RESULTS: We first studied antithrombotic potency in vitro, comparing fibrin-targeted hirudin (recombinant hirudin covalently linked to the Fab' fragment of the anti-fibrin monoclonal antibody 59D8) to recombinant hirudin in baboon plasma. Fibrin-targeted hirudin was nine times more effective than recombinant hirudin in inhibiting fibrin deposition on experimental clot surfaces in baboon plasma (P < .01). The potency of fibrin-targeted hirudin was then compared with that of recombinant hirudin in a baboon model of thrombus formation. 111In-labeled platelet deposition was measured in a synthetic graft segment of an extracorporeal arteriovenous shunt in control animals and in animals receiving either fibrin-targeted hirudin or hirudin. In these experiments, fibrin-targeted hirudin was 10-fold more potent than hirudin in inhibiting platelet deposition and thrombus formation (P < .05). CONCLUSIONS: These data indicate that targeting a thrombin inhibitors such as hirudin to an epitope present in thrombi results in increased antithrombotic potency.     

A temporal cross section specification of the demand for gasoline using a random coefficient regression model

This paper uses a random coefficient regression approach to estimate the demand for gasoline by pooling cross-sectional (state level) and time series data. The analysis proceeds by estimating two alternative models, namely a stock adjustment model and a flow adjustment model. The two models are estimated using state level data on gasoline consumption, gasoline price, income and the stock of automobiles. The random coefficient specification of each demand model is estimated assuming heteroskedastic disturbances across states, autocorrelated disturbances over time and variable intercept and slope coefficients across states. The resultant price and income elasticities are compared and inferences concerning the ability of the flow adjustment model to approximate the underlying demand function are made.     

System-specific static code analyses: a case study in the complex embedded systems domain

In this paper, we are exploring the approach to utilize system-specific static analyses of code with the goal to improve software quality for specific software systems. Specialized analyses, tailored for a particular system, make it possible to take advantage of system/domain knowledge that is not available to more generic analyses. Furthermore, analyses can be selected and/or developed in order to best meet the challenges and specific issues of the system at hand. As a result, such analyses can be used as a complement to more generic code analysis tools because they are likely to have a better impact on (business) concerns such as improving certain software quality attributes and reducing certain classes of failures. We present a case study of a large, industrial embedded system, giving examples of what kinds of analyses could be realized and demonstrate the feasibility of implementing such analyses. We synthesize lessons learned based on our case study and provide recommendations on how to realize system-specific analyses and how to get them adopted by industry.