The transformation of acute viral hepatitis B into chronic

500 patients with hepatitis B have been followed up from the acute onset to long-term outcome. As for chronic transformation of the disease, at higher risk are patients with mild form, progredient run and inadequate immune response. Of the highest value for identification and prognostication was a dynamic quantitative control over the system HBeAg-anti-HBe providing separate prognosis of establishment of replicative or integrative variants of chronic hepatitis B. In progredient infection an early administration of reaferon in combination with thymogen is thought valid which in many patients is sufficient to prevent the disease transformation into a chronic form.     

Successful ablation of atrioventricular nodal reentry tachycardia in a patient with persistent left superior vena cava

We report the case of a patient with atrioventricular nodal reentry tachycardia associated with persistent left superior vena cava draining to a large coronary sinus. Successful ablation was performed at the level of the superior lip of the coronary sinus ostium.     

Comparative modeling of substrate binding in the S1' subsite of serine carboxypeptidases from yeast, wheat, and human

Human cathepsin A ("lysosomal protective protein"; E.C.3.4.16.5) is a multifunctional lysosomal protein which forms a high-molecular-weight complex with beta-galactosidase and alpha-neuraminidase, protecting them against intralysosomal proteolysis. In addition to this protective function, cathepsin A is a serine carboxypeptidase and the understanding of its catalytic function requires a definition of its substrate specificity. For this purpose, we used a combined experimental [Pshezhetsky, A. V., Vinogradova, M. V., Elsliger, M.-A., El-Zein, F., Svedas, V.K., & Potier, M. (1995) Anal. Biochem. 230, 303-307] and theoretical approach comparing cathepsin A to two different homologous carboxypeptidases of the same family: yeast carboxypeptidase Y and wheat carboxypeptidase II. We computed the energies involved in substrate binding to the S1' subsite (C-terminal) of cathepsin A using a structural model based on the X-ray structure of the homologous wheat carboxypeptidase II. The binding energies of N-blocked Phe-Xaa dipeptide substrates to the active sites of cathepsin A, wheat carboxypeptidase II, and yeast carboxypeptidase Y were estimated using a molecular mechanics force field supplemented with a solvation energy term. This theoretical analysis showed a good correlation with the experimentally determined free energies of substrate binding. This result validates the use of this approach to analyze the energetics of substrate binding to the S1' subsite and provides a rational interpretation of serine carboxypeptidase-substrate interactions in molecular terms. We conclude that the three serine carboxypeptidases have similar affinities for substrates with hydrophobic P1' amino acid residues but that the wheat enzyme has an additional capacity for binding positively charged P1' residues. Finally, the substrate specificity of human cathepsin A is very similar to that of carboxypeptidase Y, with a high binding affinity for substrates with hydrophobic P1' residues, but the affinity of cathepsin A for P1; Phe residue is higher than for the Leu residue.     

Detecting feigned depression and schizophrenia on the MMPI-2

Increasingly, investigations evaluating the effectiveness of the MMPI-2 in the assessment of malingering employ methodologies whereby research participants are asked to feigned specific disorders rather than just to "fake bad." Yet there is little research addressing the issue of whether different validity scales and indicators work differently in the detection of different feigned disorders. In this study the comparative effectiveness of a number of validity scales and indicators on the MMPI-2 to assess feigned depression and feigned schizophrenia were evaluated. Overall, the validity scales and indicators were better at detecting feigned schizophrenia than they were in detecting feigned depression, attributable, most likely, to closer familiarity with depressive experiences. The validity scales F, Fb, and F(p) best distinguish patients with schizophrenia from participants feigning schizophrenia, and F and Fb best distinguish patients with depression from participants feigning depression.     

Direct and residual effect of fertilizer zinc application on the yield and chemical composition of rice-wheat crops in an alkali soil

A field experiment was conducted on an alkali soil to evaluate the direct and the residual effect of six levels of zinc i.e. 0, 2.25, 4.5, 9.0, 18.0 and 27.0 kg Zn ha^–1 added either once to the first crop only or continuously to each crop on the growth, yield and chemical composition of plants grown in a rice-wheat cropping sequence. The soils were amended with gypsum applied at the uniform rate of 14 t ha^–1. Zinc was supplied as zinc sulphate. Application of zinc at the rate of 2.25 kg ha^–1 to both rice and wheat crops or an annual application of 4.5 kg Zn ha^–1 only to rice was found optimum for rice-wheat sequence. Higher zinc applications increased the availability of zinc in the soil and its content in the plants but did not increase crop yield. DTPA extractable zinc build up was more for zinc applied at the rate of 2.25 kg ha^–1 to each crop compared to a single zinc application of equivalent amount. Results of these studies have shown that continuous Zn application up to 27 kg Zn ha^–1 to each crop did not induce nutrient imbalances and had no adverse effect on crop yield.