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MW Byrne 《Canadian Metallurgical Quarterly》1997,2(4):27-34
Preventive services to assist parents to understand their children's developmental needs and to devise positive, culturally appropriate strategies for managing them are not recognized as important by most third party health service payers, especially managed care programs. Room-To-Grow is an intervention designed to meet identified community needs in a culturally appropriate and sensitive way. This article discusses the evolution of this intervention and the problems encountered in finding appropriate reimbursement streams. 相似文献
73.
Probing the interactions of putidaredoxin with redox partners in camphor P450 5-monooxygenase by mutagenesis of surface residues 总被引:1,自引:0,他引:1
M Holden M Mayhew D Bunk A Roitberg V Vilker 《Canadian Metallurgical Quarterly》1997,272(35):21720-21725
The role of surface amino acid residues in the interaction of putidaredoxin (Pdx) with its redox partners in the cytochrome P450cam (CYP101) system was investigated by site-directed mutagenesis. The mutated Pdx genes were expressed in Escherichia coli, and the proteins were purified and studied in vitro. Activity of the complete reconstituted P450cam system was measured, and kinetic parameters were determined. Partial assays were also conducted to determine the effect of the mutations on interactions with each redox partner. Some mutations altered interactions of Pdx with one redox partner but not the other. Other mutations affected interactions with both redox partners, suggesting some overlap in the binding sites on Pdx for putidaredoxin reductase and CYP101. Cysteine 73 of Pdx was identified as important in the interaction of Pdx with putidaredoxin reductase, whereas aspartate 38 serves a critical role in the subunit binding and electron transfer to CYP101. 相似文献
74.
HC Kwaan M Levin S Sakurai O Kucuk MW Rooney LJ Lis JW Kauffman 《Canadian Metallurgical Quarterly》1997,26(6):1061-1068
Digital gangrene was observed in a patient who had angiographic findings of digital arterial occlusion. The patient's blood showed a marked red blood cell aggregation with rouleaux formation in long chains, which could not be dispersed at shear rates up to 200 sec-1. Studies of the patient's blood revealed the presence of an abnormal fibrinogen capable of aggregating normal red blood cells. This fibrinogen was found by Raman spectroscopy to have an increased alpha-helical content, whereas the beta-sheet content was decreased. Defibrinogenation therapy with ancrod resulted in a dramatic symptomatic relief. The disappearance of the abnormal fibrinogen 6 months later and an absence of a family history indicate that this dysfibrinogenemia was acquired. 相似文献
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T Goldman AL Hallin CM Hoffman LE Piilonen D Preston RD Bolton MD Cooper JS Frank PA Heusi GE Hogan FG Mariam HS Matis RE Mischke VD Sandberg GH Sanders U Sennhauser R Werbeck RA Williams D Grosnick SC Wright SL Wilson R Hofstadter EB Hughes MW Ritter VL Highland J McDonough 《Canadian Metallurgical Quarterly》1987,36(5):1543-1546
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