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11.
M Adamczyk J Fishpaugh PG Mattingly K Shreder 《Canadian Metallurgical Quarterly》1998,8(24):3595-3598
The synthesis, conjugation, and chemiluminescent evaluation of zero, first, and second order acridinium-based Tracermer signal generators are described. Members of this family of labels have potential use as tracers in diagnostic assays and are structurally similar to arborol dendrimers. Tracermer-BSA conjugates showed up to a sixfold increase in light emission compared to the normal acridinium label. 相似文献
12.
Luzhetskyy A Zhu L Gibson M Fedoryshyn M Dürr C Hofmann C Hoffmeister D Ostash B Mattingly C Adams V Fedorenko V Rohr J Bechthold A 《Chembiochem : a European journal of chemical biology》2005,6(4):675-678
Two genes from Streptomyces cyanogenous S136 that encode the reductase LanZ4 and the hydroxylase LanZ5, which are involved in landomycin A biosynthesis, were characterized by targeted gene inactivation. Analyses of the corresponding mutants as well as complementation experiments have allowed us to show that LanZ4 and LanZ5 are responsible for the unique C-11-hydroxylation that occurs during landomycin biosynthesis. Compounds accumulated by the lanZ4/Z5 mutants are the previously described landomycin F and the new landomycins M and O. 相似文献
13.
Mattingly M. Bailey E.A. Dutton A.W. Roemer R.B. Devasia S. 《IEEE transactions on bio-medical engineering》1998,45(9):1154-1162
Accurate thermal models are needed in hyperthermia cancer treatments for such tasks as actuator and sensor placement design, parameter estimation, and feedback temperature control. The complexity of the human body produces full-order models which are too large for effective execution of these tasks, making use of reduced-order models necessary. However, standard balanced-realization (SBR)-based model reduction techniques require a priori knowledge of the particular placement of actuators and sensors for model reduction. Since placement design is intractable (computationally) on the full-order models, SBR techniques must use ad hoc placements. To alleviate this problem, an extended balanced-realization (EBR)-based model-order reduction approach is presented. The new technique allows model order reduction to be performed over all possible placement designs and does not require ad hoc placement designs. It is shown that models obtained using the EBR method are more robust to intratreatment changes in the placement of the applied power field than those models obtained using the SBR method 相似文献
14.
SJ Mattingly 《Canadian Metallurgical Quarterly》1977,17(3):586-590
The recovery of Streptococcus mutans FA-1 in a complete, chemically defined medium was examined after 1, 3, and 6 h of essential amino acid deprivation. Amino acids could be divided into two groups based on their effect on the relative rates of recovery: those amino acids (leucine and cystine) that are precursors of protein only, and amino acids (glutamate/glutamine or lysine) that are incorporated into both protein and cell wall peptidoglycan. Culture turbidity, deoxyribonucleic acid, ribonucleic acid, protein and cell wall peptidoglycan measurements indicated rapid recovery after leucine/cystine starvation periods. However, a 6-h leucine/cystine deprivation resulted in a slower exponential rate of growth (180-min doubling time compared to the normal doubling time of 85 to 90 min) after recovery. Glutamate/glutamine starvation, on the contrary, resulted in greatly extended recovery periods, especially after 3- and 6-h amino acid deprivations. Macromolecular synthesis was most severely affected by 6-h glutamate/glutamine starvation and required 6 to 10 h for recovery of an exponential rate. A delay in the recovery of deoxyribonucleic acid and cell wall peptidoglycan synthesis beyond that of the other macromolecules was observed after 1 and 3 h of deprivation with either leucine/cystine or glutamate/glutamine. However, after a 6-h amino acid deprivation, deoxyribonucleic acid synthesis recovered more rapidly than that of the other macromolecules studied. The results are discussed in terms of the nutritional environment of the oral cavity and its effect on the growth and survival of S. mutans. 相似文献
15.
J Woodward SM Mattingly M Danson D Hough N Ward M Adams 《Canadian Metallurgical Quarterly》1996,14(7):872-874
A new in vitro enzymatic pathway for the generation of molecular hydrogen from glucose has been demonstrated. The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase. Stoichiometric yields of hydrogen were produced from glucose with the continuous recycling of cofactor. This simple system may provide a method for the biological production of hydrogen from renewable sources. In addition, the other product of this reaction, gluconic acid, is a high-value chemical commodity. 相似文献
16.
Overnight specimens of urine were collected from control, obese, and hirsute women and from 11 women with Cushing's syndrome. Urinary 11-hydroxycorticosteroid levels in the group with Cushing's syndrome were significantly higher than in the other three groups. This simple test has proved invaluable when screening for Cushing's syndrome in a busy outpatient clinic. 相似文献
17.
Hybrid optimization and Bayesian inference techniques for a non‐smooth radiation detection problem 下载免费PDF全文
Răzvan Ştefănescu Kathleen Schmidt Jason Hite Ralph C. Smith John Mattingly 《International journal for numerical methods in engineering》2017,111(10):955-982
We propose several algorithms to recover the location and intensity of a radiation source located in a simulated 250 × 180 m block of an urban center based on synthetic measurements. Radioactive decay and detection are Poisson random processes, so we employ likelihood functions based on this distribution. Owing to the domain geometry and the proposed response model, the negative logarithm of the likelihood is only piecewise continuous differentiable, and it has multiple local minima. To address these difficulties, we investigate three hybrid algorithms composed of mixed optimization techniques. For global optimization, we consider simulated annealing, particle swarm, and genetic algorithm, which rely solely on objective function evaluations; that is, they do not evaluate the gradient in the objective function. By employing early stopping criteria for the global optimization methods, a pseudo‐optimum point is obtained. This is subsequently utilized as the initial value by the deterministic implicit filtering method, which is able to find local extrema in non‐smooth functions, to finish the search in a narrow domain. These new hybrid techniques, combining global optimization and implicit filtering address, difficulties associated with the non‐smooth response, and their performances, are shown to significantly decrease the computational time over the global optimization methods. To quantify uncertainties associated with the source location and intensity, we employ the delayed rejection adaptive Metropolis and DiffeRential Evolution Adaptive Metropolis algorithms. Marginal densities of the source properties are obtained, and the means of the chains compare accurately with the estimates produced by the hybrid algorithms. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
18.
GJ Van Flandern BE Bierbaum AH Newberg SL Gomes DA Mattingly PA Karpos 《Canadian Metallurgical Quarterly》1998,13(7):804-811
In this study, 92 primary total hip arthroplasties were performed in 83 patients using a porous-coated, dual-radius, cementless, acetabular component. All hips underwent line-to-line dome reaming with press-fit implantation that was judged to have complete bone contact. This acetabular shell provides a 1-mm oversized peripheral rim, which adds excellent initial stability while allowing complete bone contact in all hips. No fractures occurred. In 83% of hips, adjunctive screw fixation was not necessary. At a minimum of 4 years, follow-up, there were no revisions, no acetabular migration, one case of acetabular erosion consistent with osteolysis, and the average Harris Hip Score was 95. The design features of this new acetabular component have provided excellent fixation with complete initial bone contact, resulting in satisfactory intermediate clinical and radiographic results. The design provides excellent peripheral stability and complete bone contact. 相似文献
19.
A series of wind tunnel tests have been conducted to examine the ways in which wind influences air infiltration energy losses in residential housing. A quantitative model for air infiltration has been developed that is based upon a linear relationship between air flow and pressure difference across walls and roof surfaces. With the model, a variety of windhouse orientations are tested, and the sheltering effects provided by solid fences, adjacent houses, and tall evergreen trees are assessed and compared. 相似文献
20.
Studied cholinergic mediation of the age-dependent improvement in response suppression of the young chick by determining the performance of 144 Vantress?×?Arbor Acre 4-day-old chicks, pretreated with scopolamine (SCO), during passive avoidance (PA) and extinction testing. In Exp I, Ss were trained to keypeck for heat reward (prepunishment training), and then tested for PA learning under immediate, 2-sec-delayed, or no shock condition. Half of the Ss in each condition received saline injections before prepunishment training and .5 mg/kg SCO injections after training. The rest received .5 mg/kg SCO injections both before and after training. For Ss in both SCO groups, delaying shock onset resulted in significantly less response suppression than immediate response-contingent shock. In Exp II, 4-day-old Ss injected with saline or SCO were trained to keypeck for heat reward and then tested for resistance to extinction under response-contingent shock or nonshock conditions. Punishment decreased the number of extinction responses for both saline and SCO groups. Results indicte that the age-dependent improvement in response suppression of the young chick cannot be explained solely by a significant increase in central cholinergic functioning. (24 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献