Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae

The widely used pESC vector series (Stratagene, La Jolla, CA, USA) with the bidirectional GAL1/GAL10 promoter provides the possibility of simultaneously expressing two different genes from a single vector in Saccharomyces cerevisiae. This system can be induced by galactose and is repressed by glucose. Since S. cerevisiae prefers glucose as a carbon source, and since its growth rate is higher in glucose than in galactose‐containing media, we compared and evaluated seven different promoters expressed during growth on glucose (pTEF1, pADH1, pTPI1, pHXT7, pTDH3, pPGK1 and pPYK1) with two strong galactose‐induced promoters (pGAL1 and pGAL10), using lacZ as a reporter gene and measuring LacZ activity in batch and continuous cultivation. TEF1 and PGK1 promoters showed the most constant activity pattern at different glucose concentrations. Based on these results, we designed and constructed two new expression vectors which contain the two constitutive promoters, TEF1 and PGK1, in opposite orientation to each other. These new vectors retain all the features from the pESC–URA plasmid except that gene expression is mediated by constitutive promoters. Copyright © 2010 John Wiley & Sons, Ltd.     

Determination and evaluation of acceptable force limits in single-digit tasks

Acceptable limits derived from psychophysical methodologies have been proposed, measured, and employed in a range of applications. There is little existing work, however, on such limits for single-digit exertions and relatively limited evidence on several fundamental issues related to data collection and processing of a sequence of self-regulated exertion levels. An experimental study was conducted using 14 male and 10 female participants (age range 18-31 years) from whom maximal voluntary exertions and maximal acceptable limits (MALs) were obtained using the index finger and thumb. Moderate to high levels of consistency were found for both measures between sessions separated by one day. Single MAL values, determined from a time series of exertions, were equivalent across three divergent processing methods and between values obtained from 5- and 25-min samples. A critical interpretation of these and earlier results supports continued use of acceptable limits but also suggests that they should be used with some caution and not equated with safe limits. This research can be applied toward future development of exertion limits based on perceived acceptability.     

Evaluation of circumferential pressure as an intervention to mitigate postural instability induced by localized muscle fatigue at the ankle

Pressure application via taping reportedly improves proprioception, and localized muscle fatigue (LMF) increases postural sway, which may be due to a loss of position sense acuity (PSA). This exploratory study investigated the effects of circumferential pressure (CP) and induced ankle LMF on sway. Fourteen young participants performed fatiguing sub-maximal isotonic plantarflexion exercises. Ankle PSA was determined, and used to categorize participants (i.e. high and low groups). Postural sway during quiet standing was assessed using a force platform, both pre- and post-LMF. CP was applied unilaterally to the dominant leg above the ankle joint. Both CP and LMF resulted in greater sway in the low PSA group. CP did not mitigate LMF effects on sway in the low PSA group, but reduced sway among the high PSA group. Reduced PSA may thus indicate less reliance on somatosensation for balance maintenance.

Relevance to industry

Potential benefits of circumferential pressure were indicated. Specifically, CP at the ankle led to improved postural control in certain individuals, which suggests that pressure application might thereby improve balance maintenance and help reduce occupational fall incidents. Results might help to design occupational interventions (specifically CP or taping) differently for individuals with low vs. high PSA.     

Fatigue during prolonged intermittent overhead work: reliability of measures and effects of working height

Shoulder pain is prevalent among industrial workers and existing evidence supports that overhead work is an important specific risk factor. Existing guidelines are limited, with overhead work typically recommended to be avoided, and research on overhead work has been mixed in terms of the effects of increasing arm reach. A laboratory-based simulation of overhead work was conducted, at three working heights, in order to facilitate improved guidelines and to identify potential non-linear effects of overhead work height. Several indicators of shoulder fatigue served as outcome measures and a preliminary study was performed to assess the reliability of several of these measures. Fatigue measures based on electromyography (EMG) generally had low reliability, whereas excellent reliability was exhibited for ratings of perceived discomfort (RPD). Consistent with this, no effects of overhead work height were found on EMG-based measures, yet clear non-linear effects were found on RPD and task performance. The source of the effects of work height appeared to be related to a combination of muscle activation levels and demands on precision/control at the highest location. These results support the utility of subjective measures for relatively low-level intermittent exertions and demonstrate increasingly detrimental fatigue and performance effects at extremes in reach during overhead work.     

Estimation of forces exerted by the fingers using standardised surface electromyography from the forearm

Determination and integration of human force capabilities and requirements is an essential component of ergonomic evaluation. With regard to hand-intensive tasks, direct force measurements can be cumbersome and intrusive. Here, the use of surface electromyography (EMG) was evaluated. EMG was obtained from three standardised electrode sites on the forearms of 30 individuals. Linear regression models were generated to estimate finger force levels from normalised electromyographic measures, while forces were generated in several finger couplings. The results suggest that standardised procedures for obtaining electromyographic data and simple linear models can be used to accurately estimate finger forces during a variety of finger exertions in fixed postures, although the level of accuracy depends on the type of model. Such models begin to overcome the limitations of direct finger strength measurements of individuals.     

The use of urea carbon 14 in breath tests as diagnostic method for Helicobacter pylori infections

The Helicobacter Pylori has been indicated as a pathologic agent in the pathogenesis of antral gastritis, gastric and duodenal ulcers and probably in gastric cancer. Due to the high incidence of this infection in our place, we decided to look for a diagnostic method quick to perform, sensitive, reliable and no invasive. The breath test of 14C urea, based in the production of urea by the H. Pylori, represents this alternative. We have found, using does of 1 microCi, a high correlation with the diagnosis of H. Pylori by biopsies. We determined as negative less than 100 DPM, doubtful between 100 and 200 DPM and positive more than 200 DPM. The high pick of the curve keeps tight relation with the degree of infection. We checked this when we suppressed the H. Pylori with bismuth subsalicylate. The breath 14C Urea test showed high sensitivity (100%) and specificity (85.71%). We conclude that this breath test is an alternative way of diagnosing and follow up for H. Pylori infections. It is highly sensitive, reproducible and no invasive.     

“高赌注”的伙伴关系：底牌在谁手里？

最近有一些科技伙伴关系,似乎很难想出哪位合作伙伴是从强势地位出牌,哪位也许是在虚张声势。如同扑克玩家们一样,科技公司为各自利益而施展全力。但是,伙伴关系经常使这种“不惜一切代价获胜”的思想变得模糊。以2005年11月底宣布的高通(Qualcomm)公司     

Reducing injuries among Native Americans: five cost-outcome analyses

This paper presents cost-outcome analyses of five injury prevention efforts in Native American jurisdictions: a safety-belt program, a streetlight project, a livestock control project, a drowning prevention program, and a suicide prevention and intervention program. Pre- and post-intervention data were analyzed to estimate projects' impact on injury reduction. Projects' costs were amortized over the time period covered by the evaluation or over the useful life of physical capital invested. Projects' savings were calculated based on estimated reduction in medical and public program expenses, on estimated decrease in lost productivity, and on estimated quality adjusted life years saved.All projects yielded positive benefit-cost ratios. The net cost per quality adjusted life years was less than zero (i.e. the monetary savings exceeded project costs) for all but one of the projects.     

摩尔定律的恩惠

半导体工业进入了利用摩尔定律所述效应--IC中集成的晶体管数每1 8个月左右翻一番--的第三个十年.事实上,元件密度更大的IC和单片系统(SoC)设计技术正在与因特网和新兴的统一网站相结合,使第三个十年成为这一丰富多彩的技术工业最激动人心的时期.