Sugarcane Serine Peptidase Inhibitors,Serine Peptidases,and Clp Protease System Subunits Associated with Sugarcane Borer (Diatraea saccharalis) Herbivory and Wounding

Sugarcane’s (Saccharum spp.) response to Diatraea saccharalis (F.) (Lepidoptera: (Crambidae) herbivory was investigated using a macroarray spotted with 248 sugarcane Expressed Sequence Tags (ESTs) encoding serine peptidase inhibitors, serine peptidases. and Clp protease system subunits. Our results showed that after nine hours of herbivory, 13 sugarcane genes were upregulated and nine were downregulated. Among the upregulated genes, nine were similar to serine peptidase inhibitors and four were similar to Bowman-Birk Inhibitors (BBIs). Phylogenetic analysis revealed that these sequences belong to a phylogenetic group of sugarcane BBIs that are potentially involved in plant defense against insect predation. The remaining four upregulated genes included serine peptidases and one homolog to the Arabidopsis AAA+ chaperone subunit ClpD, which is a member of the Clp protease system. Among the downregulated genes, five were homologous to serine peptidases and four were homologous to Arabidopsis Clp subunits (three homologous to Clp AAA+ chaperones and one to a ClpP-related ClpR subunit). Although the roles of serine peptidase inhibitors in plant defenses against herbivory have been extensively investigated, the roles of plant serine peptidases and the Clp protease system represent a new and underexplored field of study. The up- and downregulated D. saccharalis genes presented in this study may be candidate genes for the further investigation of the sugarcane response to herbivory.     

Fasting and post-methionine load homocyst(e)ine values are correlated with microalbuminuria and could contribute to worsening vascular damage in non-insulin-dependent diabetes mellitus patients

The study aim was to assess the relationship between homocyst(e)inemia and microalbuminuria in non-insulin-dependent diabetes mellitus (NIDDM) patients. The study was performed on 33 NIDDM patients (16 males and 17 females), and 16 healthy control subjects (seven males and nine females). Plasma fasting and post-methionine load homocyst(e)ine (tHcy), together with other parameters that could modify tHcy levels, were assessed. There were no significant differences between NIDDM patients and controls for fasting tHcy (8.12 +/- 3.17 v 7.19 +/- 2.40 micromol/L) and post-methionine load tHcy (26.51 +/- 11.50 v 25.06 +/- 10.76 micromol/L). Moreover, there was a significant correlation between urinary albumin excretion (UAE) and fasting tHcy (r = .340, P = .05) and post-methionine load tHcy (r = .502, P = .004) in NIDDM patients. Fasting tHcy was correlated both with post-methionine load tHcy (r = .429, P = .01) and with vitamin B12 (r = -.349, P = .04) in NIDDM patients. Microalbuminuric NIDDM patients had higher fasting tHcy (9.05 +/- 3.83 micromol/L) than normoalbuminurics (7.12 +/- 1.95 micromol/L). In addition, NIDDM patients with complications presented higher fasting tHcy values than the group without complications (9.61 +/- 3.34 v 6.53 +/- 2.09 micromol/L, Kolmogorov-Smirnov two-sample test for nonparametric data [KS] = 1.794, P = .003), without any other significant differences in the parameters considered. tHcy could be an important risk factor worsening the prognosis in NIDDM patients, especially microalbuminuric patients. Microalbuminuric NIDDM patients could be particularly prone to hyperhomocyst(e)inemia, probably due to endothelial or renal dysfunction with a reduction in the scavenging of tHcy.     

Kinetic,thermodynamic parameters and in vitro digestion of tannase from Aspergillus tamarii URM 7115

Tannase is an enzyme used in various industries and produced by a large number of microorganisms. The aim of this study was to evaluate tannase production to determine the biochemical, kinetic, and thermodynamic properties and to simulate tannase in vitro digestion. The tannase-producing fungal strain was isolated from “jamun” leaves and identified as Aspergillus tamarii. Temperature at 26°C for 67?h was the best combination for maximum tannase activity (6.35-fold; initial activity in Plackett–Burman design—15.53?U/mL and average final activity in Doehlert design—98.68?U/mL). The crude extract of tannase was optimally active at 40°C, pH 5.5 and 6.5. Moreover, tannase was stimulated by Na⁺, Ca²⁺, Mg²⁺, and Mn²⁺. The half-life at 40°C lasted 247.55?min. The free energy of Gibbs, enthalpy, and entropy, at 40°C, was 81.47, 16.85, and ?0.21?kJ/mol?·?K, respectively. After total digestion, 123.95% of the original activity was retained. Results suggested that tannase from A. tamarii URM 7115 is an enzyme of interest for industrial applications, such as gallic acid production, additive for feed industry, and for beverage manufacturing, due to its catalytic and thermodynamic properties.     

Pseudo-chronological simulation for composite reliability analysiswith time varying loads

This paper presents a new methodology to evaluate loss of load indices, with particular emphasis on LOLC (loss of load cost) assessment, for composite generation and transmission systems considering time varying loads for different areas or buses. The proposed approach, named pseudo-chronological simulation, retains the computational efficiency of nonsequential Monte Carlo simulation and the ability to model chronological load curves in sequential simulation. It considers the actual blocks of unsupplied energy per consumer class, per bus, and the respective duration, to accurately characterize the interruption process. Case studies on the IEEE-MRTS (Modified Reliability Test System) and the BSS (Brazilian South-Southeastern System) are presented and discussed     

Image analysis and rule-based reasoning for a traffic monitoringsystem

The paper presents an approach for detecting vehicles in urban traffic scenes by means of rule-based reasoning on visual data. The strength of the approach is its formal separation between the low-level image processing modules and the high-level module, which provides a general-purpose knowledge-based framework for tracking vehicles in the scene. The image-processing modules extract visual data from the scene by spatio-temporal analysis during daytime, and by morphological analysis of headlights at night. The high-level module is designed as a forward chaining production rule system, working on symbolic data, i.e., vehicles and their attributes (area, pattern, direction, and others) and exploiting a set of heuristic rules tuned to urban traffic conditions. The synergy between the artificial intelligence techniques of the high-level and the low-level image analysis techniques provides the system with flexibility and robustness     

Correlates of motorcycle helmet use among recent graduates of a motorcycle training course

Helmets significantly decrease morbidity and mortality from motorcycle crashes, but many areas of the world lack universal helmet laws. To educate motorcyclists in areas without helmet laws, more knowledge of motorcyclists’ helmet beliefs is needed.A web-based survey was therefore designed to assess motorcyclists’ attitudes, norms and behaviors towards helmets in a U.S. state with a limited helmet law. Of 445 survey respondents, 68.4% of respondents reported always wearing a helmet. The not-always-helmeted riders were more likely than the always-helmeted to be male; to bave less education; and to have a history of previous motorcycle crashes and injuries. Although both groups had taken rider training classes, fewer of the not-always-helmeted had learned how to ride in a class. The strongest correlates of being not-always-helmeted (vs. always-helmeted) were attitudes that helmets were not protective and impaired sight/hearing; and the normative belief that they would only wear helmets if forced by law. Because attitudes are often more easily changed than normative beliefs, education may increase helmet use. However, less than half of riders in this state with a mandatory education program learned how to ride from a rider education course, and 44% of non-helmeted said they would only wear a helmet if forced by law. Legislation may therefore be a more efficient and effective strategy than education to increase helmet use.     

Surface spin slips in thin dysprosium films

We report a theoretical investigation of new magnetic phases of thin dysprosium films. At the surfaces, the balance between exchange and anisotropy energies favors the alignment of spins along the basal plane easy axis directions. Near the Curie temperature, the helimagnetic phase is strongly modified, allowing surface spins to lock into the basal plane anisotropy easy directions. As the temperature raises, we find transitions to other surface lockin patterns, which are tunable by external field strengths of a few hundred milli-Oersteds.     

Mechanical countermeasures to subsynchronous torsional instability[in turbogenerators]

A mechanical countermeasure is presented that uses a fluid coupling to drive a flywheel at the end of a turbogenerator shaft, resulting in increased torsional damping for the shaft. The effect of varying the flywheel inertia and the fluid coupling's characteristics on the mechanical damping is presented. The application of such a countermeasure to subsynchronous resonance requires studies to determine the size of the coupling and flywheel that would be required to offset any negative electrical damping that may exist due to series compensation on the transmission system     

Impact of maternal alcoholism on separation of children from their mothers: findings from a sample of incarcerated women

Research on achievement goal orientation in sport has relied primarily on traditional statistical methodology to examine group mean differences. Unfortunately, examination of the measurement model is generally ignored prior to means testing. This study reports an application of structural equation modeling (SEM) in testing measurement invariance and latent mean structure of the Task and Ego Orientation in Sport Questionnaire (TEOSQ; Duda & Nicholls, 1989) using male and female college students. A confirmatory factor analysis for testing invariance revealed invariant measurement properties and factor structures across gender, indicating that task and ego orientation are similarly conceptualized by male and female students. Subsequent testing of latent mean structures, however, showed significant gender differences with respect to ego orientation, but no difference in task orientation. The SEM procedures used in the present study demonstrate additional construct validity and internal consistency reliability for the TEOSQ and, by confirming its factor structure, provide a sound psychometric basis for its continued use in substantive studies focusing on the comparison of achievement goal orientation across gender.     

Dwell-Fatigue of Ni-Based Superalloys with Serrated and Planar Grain Boundary Morphologies: The Role of the γ′ Phase on Strain Accumulation and Cavitation

The effect of grain boundary (GB) morphology on the cavitation behavior in a Ni-based superalloy, RR1000, was studied during elevated temperature dwell-fatigue at 700 °C. Following a solution heat treatment, the material was control cooled at two different rates, resulting in high angle GB morphologies that were tailored as either serrated or planar. The resulting γ′ precipitate structures were characterized near GBs and within grains. Along serrated GBs coarsened and elongated γ′ precipitates formed and consequently created adjacent regions that were denuded of γ′ precipitates. Cyclic dwell-fatigue experiments were performed at low and high stress amplitudes to vary the amount of imparted strain on the specimens. A combination of electron backscatter diffraction and digital image correlation were used to resolve strain localization relative to the GBs, in which strain accumulation was found to precede cavity formation. Additionally, the regions denuded of the γ′ precipitates were observed to localize strain and to be initial sites of cavitation. These results present a quantitative strain analysis between two variants of an RR1000 alloy, which provides the micromechanical rationale to assess the increased proclivity for serrated GBs to form cavities.