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排序方式: 共有790条查询结果,搜索用时 46 毫秒
71.
Ivan Rehor Jitka Slegerova Jan Kucka Vladimir Proks Vladimira Petrakova Marie‐Pierre Adam François Treussart Stuart Turner Sara Bals Pavel Sacha Miroslav Ledvina Amy M. Wen Nicole F. Steinmetz Petr Cigler 《Small (Weinheim an der Bergstrasse, Germany)》2014,10(6):1106-1115
High pressure high temperature (HPHT) nanodiamonds (NDs) represent extremely promising materials for construction of fluorescent nanoprobes and nanosensors. However, some properties of bare NDs limit their direct use in these applications: they precipitate in biological solutions, only a limited set of bio‐orthogonal conjugation techniques is available and the accessible material is greatly polydisperse in shape. In this work, we encapsulate bright 30‐nm fluorescent nanodiamonds (FNDs) in 10–20‐nm thick translucent (i.e., not altering FND fluorescence) silica shells, yielding monodisperse near‐spherical particles of mean diameter 66 nm. High yield modification of the shells with PEG chains stabilizes the particles in ionic solutions, making them applicable in biological environments. We further modify the opposite ends of PEG chains with fluorescent dyes or vectoring peptide using click chemistry. High conversion of this bio‐orthogonal coupling yielded circa 2000 dye or peptide molecules on a single FND. We demonstrate the superior properties of these particles by in vitro interaction with human prostate cancer cells: while bare nanodiamonds strongly aggregate in the buffer and adsorb onto the cell membrane, the shell encapsulated NDs do not adsorb nonspecifically and they penetrate inside the cells. 相似文献
72.
Data from total synchronous fluorescence spectroscopy (TSFS) measurements of normal and malignant breast tissue samples are introduced in supervised self-organizing maps, a type of artificial neural network (ANN), to obtain diagnosis. Three spectral regions in both TSFS patterns and first-derivative TSFS patterns exhibited clear differences between normal and malignant tissue groups, and intensities measured from these regions served as inputs to neural networks. Histology findings are used as the gold standard to train self-organizing maps in a supervised way. Diagnostic accuracy of this procedure is evaluated with sample test groups for two cases, when the neural network uses TSFS data and when the neural network uses data from first-derivative TSFS. In the first case diagnostic sensitivity of 87.1% and specificity of 91.7% are found, while in the second case sensitivity of 100% and specificity of 94.4% are achieved. 相似文献
73.
The present investigation deals with the effect of Al2O3 particle reinforcement on the lubricated sliding behavior of ZA-27 alloy. The composites with 3, 5, and 10 wt% of Al2O3 particles were produced by the compocasting procedure. Tribological properties of alloy and composites were studied, using
block-on-disk tribometer at different specific loads and sliding speeds. The test results revealed that composite specimens
exhibited significantly lower wear rate, but higher coefficient of friction than the matrix alloy specimens in all the combinations
of applied loads and sliding speeds. The improved antiwear characteristics of the composites were influenced by positive effects
of higher frictional heating on compatibility of the composite phases and suppressing micro-cracking tendency. Due to that,
effects of reinforcing hard particles were manifested through the reduced wear rate of composites, especially in conditions
of higher load, lower sliding speeds and higher Al2O3 particle content. In present wear tests, the significant forming of mechanically mixed layers was not noticed, what is confirmed
by the SEM microphotographs. 相似文献
74.
75.
Miroslav Chomat Daniela Berkova Vlastimil Matejec Ivan Kasik Gabriela Kuncova 《Sensors and actuators. B, Chemical》2003,90(1-3):151-156
This paper deals with the effect of hydrodynamic conditions on the detection of toluene dissolved in water by means of fibers with a truncated parabolic inverted-graded index (IGI) profile. The detection is based on refractive-index changes of a detection layer of polydimethylsiloxane (PDMS) applied on the fiber which are induced by penetration of toluene into the layer. The dependence of the response of the sensing fiber placed in a flow cell on the flow velocity of the detected aqueous solution of toluene has been investigated. The sensing fiber was successively exposed to flows of distilled water and aqueous solutions of toluene. The fiber responses were measured for three chosen concentrations of the toluene solutions and for five flow velocities of the solutions. From these results the dependence of the relative decrease of the output signal on the flow velocity was determined. It has been found that the magnitude of output signal changes is approximately proportional to the solution concentration and the rate of the signal changes in the tested concentration range depends approximately linearly on the solution flow velocity. 相似文献
76.
Towards fully probabilistic control design 总被引:2,自引:0,他引:2
Miroslav Kárný 《Automatica》1996,32(12):1719-1722
77.
Bituminous coals produced in the Ostrava—Karviná coal basin show considerable variation in their maceral composition and rank. In order to explain the significance of the petrographic composition, structure and chemical parameters in coal degradation processes, a large number of coal samples was investigated. From the relationships obtained, the behaviour of macerals during these processes is discussed. It is found that isometamorphic vitrinites are characterized by a distinctive behaviour affected to a first approximation by the atomic ratios C/H and C/O. Inertinite, despite its occasional significantly higher volatile matter, does not yield an appreciable amount of fused material. 相似文献
78.
For the problem of stabilization of nonlinear systems linear in unknown constant parameters, we introduce the concept of an adaptive control Lyapunov function (aclf) and use Sontag's constructive proof of Artstein's theorem to design an adaptive controller. In this framework the problem of adaptive stabilization of a nonlinear system is reduced to the problem of nonadaptive stabilization of a modified system. To illustrate the construction of aclf's we give an adaptive backstepping lemma which recovers our earlier design. 相似文献
79.
Trimethylammonium-2-hydroxypropyl (TMAHP) derivatives of spruce wood meal (SWM) and holocellulose of this specia were prepared by the reaction of wood meal with 3-chlor-2-hydroxy-propyltrimethylammoniumchloride (CHMAC) in alkaline medium. The TMAHP samples were fractionated and yields and exchange capacity (Q) of individual fractions were compared with beech and aspen fractions obtained under the same conditions. As it is evident from 13C-NMR spectroscopy and GPC analysis the water soluble fraction from TMAHP–SWM consists only of lignin–saccharide degradation products. The NaOH extracts of TMAHP–SWM and TMAHP–holocellulose as well as the water-soluble fraction from TMAHP–holocellulose are polymeric materials. From TMAHP–SWM only 3.1% of alkali-soluble material could be extracted, while from TMAHP–holocellulose 15.7% of water-soluble and 7.9% of alkali-soluble materials were obtained. 相似文献
80.