全文获取类型
收费全文 | 2386篇 |
免费 | 88篇 |
国内免费 | 61篇 |
专业分类
电工技术 | 62篇 |
综合类 | 138篇 |
化学工业 | 122篇 |
金属工艺 | 58篇 |
机械仪表 | 51篇 |
建筑科学 | 46篇 |
矿业工程 | 15篇 |
能源动力 | 28篇 |
轻工业 | 112篇 |
水利工程 | 25篇 |
石油天然气 | 9篇 |
武器工业 | 4篇 |
无线电 | 76篇 |
一般工业技术 | 78篇 |
冶金工业 | 1586篇 |
原子能技术 | 8篇 |
自动化技术 | 117篇 |
出版年
2024年 | 5篇 |
2023年 | 17篇 |
2022年 | 36篇 |
2021年 | 39篇 |
2020年 | 33篇 |
2019年 | 17篇 |
2018年 | 13篇 |
2017年 | 22篇 |
2016年 | 25篇 |
2015年 | 27篇 |
2014年 | 37篇 |
2013年 | 43篇 |
2012年 | 67篇 |
2011年 | 70篇 |
2010年 | 70篇 |
2009年 | 74篇 |
2008年 | 70篇 |
2007年 | 80篇 |
2006年 | 73篇 |
2005年 | 43篇 |
2004年 | 36篇 |
2003年 | 30篇 |
2002年 | 24篇 |
2001年 | 31篇 |
2000年 | 16篇 |
1999年 | 50篇 |
1998年 | 464篇 |
1997年 | 294篇 |
1996年 | 169篇 |
1995年 | 97篇 |
1994年 | 71篇 |
1993年 | 91篇 |
1992年 | 16篇 |
1991年 | 7篇 |
1990年 | 13篇 |
1989年 | 7篇 |
1988年 | 10篇 |
1987年 | 6篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1981年 | 13篇 |
1980年 | 16篇 |
1979年 | 1篇 |
1978年 | 9篇 |
1977年 | 48篇 |
1976年 | 131篇 |
1975年 | 6篇 |
1955年 | 2篇 |
排序方式: 共有2535条查询结果,搜索用时 15 毫秒
11.
12.
TK Cherniaeva NA Matveeva IuG Kuzmichev MP Gracheva 《Canadian Metallurgical Quarterly》1997,36(3):26-28
Nuchal screening has the following advantages: Non invasive. First trimester procedure. Results are obtained quickly. 80-90% detection rate for Down's Syndrome. 相似文献
13.
SF Ding JD Delhanty JS Dooley L Bowles CB Wood NA Habib 《Canadian Metallurgical Quarterly》1993,17(3):405-409
We have previously shown that the tumor suppressor gene for hepatocellular carcinoma (HCC) without cirrhosis may be located on chromosome 5q35-qter. In this study, we analyzed nine cases of primary HCC without cirrhosis using probes from the MCC and APC genes, which are in the region 5q21-22. None of the informative cases had allele loss detected by these probes, whereas the probe lambda MS8 for the region 5q35-qter showed allele loss in six out of six informative cases. The results confirm that the putative tumor suppressor gene for HCC without cirrhosis on chromosome 5q is distinct from the MCC and APC genes. 相似文献
14.
15.
DJ Benos BH Hahn JK Bubien SK Ghosh NA Mashburn MA Chaikin GM Shaw EN Benveniste 《Canadian Metallurgical Quarterly》1994,91(2):494-498
Infection by human immunodeficiency virus type 1 (HIV-1) is often complicated by a variety of neurological abnormalities. The most common clinical syndrome, termed acquired immunodeficiency syndrome (AIDS) dementia complex, presents as a subcortical dementia with cognitive, motor, and behavioral disturbances and is unique to HIV-1 infection. The pathogenesis of this syndrome is poorly understood but is believed to involve interactions among virally infected macrophages/microglia, astrocytes, and neurons. In this study, we show that exposure of primary rat and human astrocytes to heat-activated HIV-1 virions, or to eukaryotically expressed HIV-1 and HIV-2 envelope glycoproteins (gp120) stimulates amiloride-sensitive Na+/H+ antiport, potassium conductance, and glutamate efflux. These effects are blocked specifically by amiloride, an inhibitor of Na+/H+ antiport and by the selective removal of gp120 with immobilized monoclonal antibody. As a result of modulation of astrocytic function by gp120, the ensuing neuronal depolarization and glutamate exposure could activate both voltage-gated and N-methyl-D-aspartate-regulated Ca2+ channels, leading to increases in intraneuronal Ca2+ and neuronal death. These findings implicate the astrocyte directly in the pathogenesis of AIDS dementia complex. 相似文献
16.
17.
Insulin-like growth factor I activates the invasion suppressor function of E-cadherin in MCF-7 human mammary carcinoma cells in vitro 总被引:1,自引:0,他引:1
ME Bracke BM Vyncke EA Bruyneel SJ Vermeulen GK De Bruyne NA Van Larebeke K Vleminckx FM Van Roy MM Mareel 《Canadian Metallurgical Quarterly》1993,68(2):282-289
The calcium-dependent cell-cell adhesion molecule E-cadherin has been shown to counteract invasion of epithelial neoplastic cells. Using three monoclonal antibodies, we have demonstrated the presence of E-cadherin at the surface of human MCF-7/6 mammary carcinoma cells by indirect immunofluorescence coupled to flow cytometry and by immunocytochemistry. Nevertheless, MCF-7/6 cells failed to aggregate in a medium containing 1.25 mM CaCl2, and they were invasive after confrontation with embryonic chick heart fragments in organ culture. Treatment of MCF-7/6 cells with 0.5 microgram ml-1 insulin-like growth factor I (IGF-I) led to homotypic aggregation within 5 to 10 min and inhibited invasion in vitro during at least 8 days. The effect of IGF-I on cellular aggregation was insensitive to cycloheximide. However, monoclonal antibodies that interfered with the function of either the IGF-I receptor (alpha IR3) or E-cadherin (HECD-1, MB2) blocked the effect of IGF-I on aggregation. The effects of IGF-I on aggregation and on invasion could be mimicked by 1 microgram ml-1 insulin, but not by 0.5 microgram ml-1 IGF-II. The insulin effects were presumably not mediated by the IGF-I receptor, since they could not be blocked by an antibody against this receptor (alpha IR3). Our results indicate that IGF-I activates the invasion suppressor role of E-cadherin in MCF-7/6 cells. 相似文献
18.
19.
NA Nathan 《Canadian Metallurgical Quarterly》1993,99(6):721-725
The application of fine-needle aspiration cytology for the diagnosis of solid lesions is well-established. Its sensitivity as a diagnostic tool for both benign and malignant lesions can vary significantly, however, and depends on the quality of the smears submitted. In particular, air-drying artifact can render wet alcohol-fixed smears unsatisfactory for diagnosis. This study examines the basic cause of air-drying artifacts, which is directly related to the moisture of the aspirate that varies among tissues and specimens from any tumor type. A simple modification to the standard smearing technique incorporating precoated slides that introduces moisture into the aspirate is recommended. The technique is applicable to aspirates from all sites without compromising accepted diagnostic cytologic criteria, and offers excellent cytologic clarity with routine stains as well as being flexible enough for other investigative staining methods. 相似文献
20.