The Arabidopsis CHL1 (AtNRT1) gene confers sensitivity to the herbicide chlorate and encodes a nitrate-regulated nitrate transporter. However, how CHL1 participates in nitrate uptake in plants is not yet clear. In this study, we examined the in vivo function of CHL1 with in vivo uptake measurements and in situ hybridization experiments. Under most conditions tested, the amount of nitrate uptake by a chl1 deletion mutant was found to be significantly less than that of the wild type. This uptake deficiency was reversed when a CHL1 cDNA clone driven by the cauliflower mosaic virus 35S promoter was expressed in transgenic chl1 plants. Furthermore, tissue-specific expression patterns showed that near the root tip, CHL1 mRNA is found primarily in the epidermis, but further from the root tip, the mRNA is found in the cortex or endodermis. These results are consistent with the involvement of CHL1 in nitrate uptake at different stages of root cell development. A functional analysis in Xenopus oocytes indicated that CHL1 is a low-affinity nitrate transporter with a K(m) value of approximately 8.5 mM for nitrate. This finding is consistent with the chlorate resistance phenotype of chl1 mutants. However, these results do not fit the current model of a single, constitutive component for the low-affinity uptake system. To reconcile this discrepancy and the complex uptake behavior observed, we propose a "two-gene" model for the low-affinity nitrate uptake system of Arabidopsis. 相似文献
Tests comparing image sets can play a critical role in PET research, providing a yes-no answer to the question "Are two image sets different?" The statistical goal is to determine how often observed differences would occur by chance alone. We examined randomization methods to provide several omnibus test for PET images and compared these tests with two currently used methods. In the first series of analyses, normally distributed image data were simulated fulfilling the requirements of standard statistical tests. These analyses generated power estimates and compared the various test statistics under optimal conditions. Varying whether the standard deviations were local or pooled estimates provided an assessment of a distinguishing feature between the SPM and Montreal methods. In a second series of analyses, we more closely simulated current PET acquisition and analysis techniques. Finally, PET images from normal subjects were used as an example of randomization. Randomization proved to be a highly flexible and powerful statistical procedure. Furthermore, the randomization test does not require extensive and unrealistic statistical assumptions made by standard procedures currently in use. 相似文献
The intersecting path is an important tool path generation method. This paper proposes an approach for the quart-parametric interpolation of intersecting paths. The objective of our approach is that the intersecting paths for surface machining can be directly interpolated within the computer numerical control (CNC) system. This enables the CNC interpolator to process the intersecting paths without geometric approximation as in existing approaches and take into consideration any specific feedrate profiles and further machining dynamical issues along the path.The interpolation of the intersection of two general parametric surfaces is transferred into interpolation of its projection curves and the time trajectories of four parameters along the intersecting curves are obtained. Our strategy is to carry out the quart-parametric interpolation based on the projection interpolation. The feedrate control method is developed, and then the interpolation algorithms for two projection curves are proposed. An error reduction scheme is presented to alleviate point deviation from the drive parametric surface. Simulations of quart-parametric interpolation have been carried out to verify the effectiveness of the proposed algorithm. 相似文献
Augmented reality (AR) has received much attention in the cultural heritage domain as an interactive medium for requesting and accessing information regarding heritage sites. In this study, we developed a mobile AR system based on Semantic Web technology to provide contextual information about cultural heritage sites. Most location-based AR systems are designed to present simple information about a point of interest (POI), but the proposed system offers information related to various aspects of cultural heritage, both tangible and intangible, linked to the POI. This is achieved via an information modeling framework where a cultural heritage ontology is used to aggregate heterogeneous data and semantically connect them with each other. We extracted cultural heritage data from five web databases and modeled contextual information for a target heritage site (Injeongjeon Hall and its vicinity in Changdeokgung Palace in South Korea) using the selected ontology. We then implemented a mobile AR application and conducted a user study to assess the learning and engagement impacts of the proposed system. We found that the application provides an agreeable user experience in terms of its affective, cognitive, and operative features. The results of our analysis showed that specific usage patterns were significant with regard to learning outcomes. Finally, we explored how the study’s key findings can provide practical design guidance for system designers to enhance mobile AR information systems for heritage sites, and to show system designers how to support particular usage patterns in order to accommodate specific user experiences better.
Intelligence has been an object of study for a long time. Different architectures try to capture and reproduce these aspects into artificial systems (or agents), but there is still no agreement on how to integrate them into a general framework. With this objective in mind, we propose an architectural methodology based on the idea of intentional configuration of behaviors. Behavior‐producing modules are used as basic control components that are selected and modified dynamically according to the intentions of the agent. These intentions are influenced by the situation perceived, knowledge about the world, and internal variables that monitor the state of the agent. The architectural methodology preserves the emergence of functionality associated with the behavior‐based paradigm in the more abstract levels involved in configuring the behaviors. Validation of this architecture is done using a simulated world for mobile robots, in which the agent must deal with various goals such as managing its energy and its well‐being, finding targets, and acquiring knowledge about its environment. Fuzzy logic, a topologic map learning algorithm, and activation variables with a propagation mechanism are used to implement the architecture for this agent. 相似文献
Spindle speed variation is a well known technique to suppress regenerative machine tool vibrations, but it is usually considered to be effective only for low spindle speeds. In the current paper, spindle speed variation is applied to the high speed milling process, at the spindle speeds where the constant speed cutting results in period doubling chatter. The stability analysis of triangular and sinusoidal shape variations is made numerically with the semi-discretization method. It is shown that the milling process can be stabilized by increasing the amplitude of the spindle speed variation, while the frequency of the variation has no significant effect on the dynamic behaviour. The results are validated by experiments. Based on the analysis of the machined workpieces, it is shown that the surface roughness can also be decreased by the spindle speed variation technique. 相似文献