Thermostable archaeal O6-alkylguanine-DNA alkyltransferases

Archaea represent some of the most ancient organisms on earth, and they have relatively uncharacterized DNA repair processes. We now show, using an in vitro assay, that extracts of two Crenarchaeota (Sulfolobus acidocaldarius and Pyrobaculum islandicum) and two Euryarchaeota (Pyrococcus furiosus and Thermococcus litoralis) contain the DNA repair protein O6-alkylguanine-DNA alkyltransferase (ATase). The ATase activities found in the archaea were extremely thermostable, with half-lives at 80 degreesC ranging from 0.5 hr (S. acidocaldarius) to 13 hr (T. litoralis). The temperature optima of the four proteins ranged from approximately 75 to approximately 100 degreesC, although activity was seen at 37 degreesC, the temperature optimum of the Escherichia coli and human ATases. In all cases, preincubaton of extracts with a short oligonucleotide containing a single O6-methylguanine residue caused essentially complete loss of ATase activity, suggesting that the alkylphosphotriester-DNA alkyltransferase activity seen in some prokaryotes is not present in Archaea. The ATase from Pyrobaculum islandicum had an apparent molecular mass of 15 kDa, making it the smallest of these proteins so far described. In higher organisms, ATase is responsible for the repair of toxic and mutagenic O6-alkylguanine lesions in alkylated DNA. The presence of ATase in these primitive organisms therefore suggests that endogenous or exogenous exposure to agents that generate appropriate substrates in DNA may be an early event in evolution.     

A study of the mechanical and electrical properties of a polymer/carbon black binder system used in battery electrodes

The effects of carbon black content and crosslinking on the mechanical and electrical properties of a fluorinated elastomer, FC2178 (Dyneon Corp., Oakdale, MN), poly(vinylidene fluoride‐co‐hexafluoropropylene), were investigated and compared to those of poly(vinylidene fluoride) (PVDF). Attention was given to changes in mechanical and electrical properties of the polymers when under cyclic deformation. To describe the mechanical properties of the carbon‐filled polymers in a way that is independent of the chemical details, two mechanical models were used to fit cyclic stress–strain experiments. The linear model was used to determine the effect of crosslinking on the mechanical properties of crosslinked FC2178 films. However, when carbon black was added to the polymer films, the linear model no longer fit the data well. In particular, the cyclic stress–strain curves for carbon‐filled polymers showed non‐linear regions and displayed the characteristic of ‘memory.’ A non‐linear element was added in parallel with the existing elements of the linear model to successfully describe the effects of the added carbon black. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 90: 1891–1899, 2003     

Effect of Drying Conditions and Level of Condensed Distillers Solubles on Protein Quality of Wheat Distillers Dried Grain with Solubles

Reduced protein quality is one of the concerns currently confronting the supply and utilization of wheat distillers dried grain with solubles (DDGS) as an animal feed ingredient. This study assessed the protein quality of wheat DDGS, expressed as acid detergent insoluble crude protein (ADICP) and lysine content, by blending wet distillers grain (WDG) with varying condensed distillers solubles (CDS) levels and drying using forced air convection, microwave, and microwave–convection methods. As the CDS level was increased, the protein content of wheat DDGS generated from the three drying methods increased. Interactions of CDS level with drying air temperature, microwave power, and microwave–convection settings had a significant effect (p < 0.05) on average ADICP and lysine contents. Higher ADICP and lower lysine contents were observed in samples dried at higher temperature, microwave power, and microwave convection settings. Further, the CDS level significantly affected the color parameters of microwave- and microwave–convection-dried samples and the drying air temperature–CDS level interaction significantly affected the color of forced air convection–dried samples. Significant lysine content–redness, ADICP–lightness color parameter, and ADICP–total color difference correlations were found in forced air convection–, microwave-, and microwave–convection-dried samples, respectively. Microwave and microwave–convection drying achieved desirable protein quality associated with low-temperature drying at much shorter times.     

Application of Multivariate Data Analysis for Assessing the Early Fate of Petrogenic Compounds in the Marine Environment Following the Baltic Carrier Oil Spill

Multivariate techniques have only rarely been used when considering the fate of complex chemical mixtures in the environment. In this article, a multivariate decomposition method (principal component analysis) has been applied together with univariate methods to assess the early fate of petrogenic compounds in the marine environment following the Baltic Carrier oil spill, Grønsund, Denmark, March 29, 2001. The chemical composition of oil on the beach has changed considerably 138 days after the accident. Both evaporation and degradation are found and the introduction of hydrocarbon degrading bacteria in a laboratory experiment increased the degradation of both PACs and aliphatic compounds. The application of principal component analysis shows promising results, calling for further use of multivariate decomposition or even regression techniques in fate studies.     

Crosslinking of polybenzimidazole membranes by divinylsulfone post‐treatment for high‐temperature proton exchange membrane fuel cell applications

Phosphoric acid‐doped polybenzimidazole (PBI) has been suggested as a promising electrolyte for proton exchange membrane fuel cells operating at temperatures up to 200 °C. This paper describes the development of a crosslinking procedure for PBI membranes by post‐treatment with divinylsulfone. The crosslinking chemistry was studied and optimized on a low‐molecular‐weight model system and the results were used to optimize the crosslinking conditions of PBI membranes. The crosslinked membranes were characterized with respect to chemical and physiochemical properties, showing improved mechanical strength and oxidative stability compared with their linear analogues. Fuel cell tests were further conducted in order to demonstrate the feasibility of the crosslinked membranes. Copyright © 2011 Society of Chemical Industry     

New dendrimer-peptide host-guest complexes: towards dendrimers as peptide carriers

Adamantyl urea and adamantyl thiourea modified poly(propylene imine) dendrimers act as hosts for N-terminal tert-butoxycarbonyl (Boc)-protected peptides and form chloroform-soluble complexes. Investigations with NMR spectroscopy show that the peptide is bound to the dendrimer by ionic interactions between the dendrimer outer shell tertiary amines and the C-terminal carboxylic acid of the peptide, and also through host-urea to peptide-amide hydrogen bonding. The hydrogen-bonding nature of the peptide-dendrimer interactions was further confirmed by using Fourier transform IR spectroscopy, for which the NH- and CO-stretch signals of the peptide amide moieties shift towards lower wavenumbers upon complexation with the dendrimer. Spatial analysis of the complexes with NOESY spectroscopy generally shows close proximity of the N-terminal Boc group of the peptide to the peripheral adamantyl groups on the dendrimer host. The influence of side-chain motif on interactions with the host is analyzed by using seven different N-Boc-protected tripeptides as guests for the dendrimer. Downfield shifts of up to 1.3 ppm were observed for the guest amide NH-proton signals. These shifts decreased with increasing 'bulkiness' of the amino acid side chains. Despite this, the dendrimer was capable of making multiple peptide-dendrimer complexes when presented with a library of seven peptides. The different peptides were all present in the host, which did not show specific preferences, and could be released under mild acidic conditions. These results show the general nature of the peptide-dendrimer interactions in the formation of either single- or multiple-peptide-dendrimer complexes.     

Combined TPS, XPS, EXAFS, and NO-TPD study of the sulfiding of Mo/Al2O3

The sulfiding of Mo/Al₂O₃ in H₂S/Ar versus in H₂S/H₂ has been studied by temperature-programmed sulfiding (TPS), X-ray photon electron spectroscopy (XPS), extended X-ray absorption fine structure (EXAFS), and temperature-programmed desorption of NO (NO-TPD). All the applied techniques agree on the sulfur content in the sulfided catalysts and the findings are in accord with a model for the H₂S production reaction. The nucleation and growth of well-ordered MoS₂ clusters are probed by XPS during sulfiding with and without the presence of hydrogen. The resulting dispersion of the MoS₂ phase is evaluated on the basis of XPS, EXAFS, and NO-TPD, and is found to be highest when the sulfiding occurs in the presence of hydrogen.     

Effect of catalyst type on the kinetics of the photoelectrochemical disinfection of water inoculated with E. coli

The rates of the photoelectrochemical disinfection of Escherichia coli at TiO₂ electrodes were measured as a function of concentration and applied potential. Two different TiO₂ photoelectrodes were used: a thermally treated titanium plate and a porous film prepared by a sol–gel hydrolysis technique. The kinetics of the disinfection process were found to depend upon the nature of the electrode material. For the thermal film they were first order, and half order for the sol–gel film. It was also found that the catalytic activity per unit surface area of catalyst is many orders of magnitude greater than that observed using TiO₂ slurries; this was attributed to the reduced rate of electron-hole recombination afforded by the application of a small potential bias (1 V vs Ag/AgCl), and hence the exploitation of the electric field enhancement (EFE) effect.     

Marine n-3 fatty acids: Basic features and background

There is some evidence from epidemiology that intake of n-3 polyunsaturated fatty acids (PUFA) from seafood may protect against coronary artery disease (CAD). This hypothesis is further supported from animal data showing a beneficial effect of n-3 PUFA on thrombosis and atherosclerosis in animals fed fish oils in most, but not all, studies. There are several mechanisms by which an increased intake of marine n-3 PUFA may protect against CAD; the most universal finding is a reduction of plasma triglycerides. It is puzzling, however, that a very low amount of n-3 PUFA, with no known beneficial biochemical effects, seems to be cardioprotective. It has therefore been of paramount interest to perform clinical trials. Such evidence and trials are discussed in later chapters, and the results have been very encouraging.     

n−3 fatty acids,heart rate variability,and sudden cardiac death

Sudden cardiac death (SCD) is a major cause of mortality in Western countries. Furthermore, SCD is often the first manifestation of coronary artery disease, making it difficult to prevent. Heart rate variability (HRV), which can be determined by extended recording of the heart rate by 24-h Holter monitoring, has been shown to be one of the best predictors of the risk of SCD. There is increasing evidence from animal experiments and clinical trials in humans that n?3 fatty acids reduce the risk of SCD. We have studied the effect of n?3 fatty acids on HRV and present data clearly showing that n?3 fatty acids increase HRV. This adds further to the hypothesis that an increased intake of n?3 fatty acids may reduce the risk of SCD.