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The glnA gene from the human pathogen Streptococcus agalactiae was cloned from a genomic library prepared with the lambda phage vector lambdaDASHII. A 4.6-kb DNA fragment of one of the recombinant phages was subcloned in pUC18. This Escherichia coli clone expressed a 52-kDa protein encoded by a 1,341-bp open reading frame. The nucleotide sequence of the open reading frame and the deduced amino acid sequence shared a significant degree of homology with the sequences of other glutamine synthetases (GS). The highest homology was between our deduced protein and GS of gram-positive bacteria such as Bacillus subtilis, Bacillus cereus, and Staphylococcus aureus. Plasmids with the cloned streptococcal glnA were able to complement E. coli glnA mutants grown on minimal media. Rabbit antisera to streptococcal GS recombinant protein recognized not only the recombinant protein but also a similar-sized band in mutanolysin extracts of all group B streptococcal strains tested, regardless of polysaccharide type or surface protein profile. The amino acid sequence of the deduced protein had similarities to other streptococcal cell-surface-bound proteins. The possible functional role of the immunological features of streptococcal GS is discussed. 相似文献
33.
ZB Tokarskaya ND Okladnikova ZD Belyaeva EG Drozhko 《Canadian Metallurgical Quarterly》1997,73(6):899-905
Dose-response relationships for alpha-radiation-induced lung cancers (adenocarcinoma, squamous carcinoma and small cell carcinoma) were developed by multifactorial analysis using data for Mayak nuclear enterprise workers chronically exposed by inhalation to 239Pu. The three most important lung cancer risk factors (smoking, plutonium incorporation, and external gamma irradiation), out of six factors previously identified, were used. Relative risks (odds ratios) were determined for 500 nuclear enterprise workers (162 cancer cases, 338 control) for different dose levels using a case-control study design and logistic regression. A threshold at about 3.7 kBq or 0.80 Gy was discovered for incorporated plutonium, which is satisfactorily described by linear-quadratic and quadratic models. Excess relative risk was 0.020 kBq(-2) and 0.97 Gy(-2). This quadratic function was mainly due to adenocarcinoma. A trend for decreasing risk was noted for the lowest levels of plutonium incorporation, near permissible level. No clear-cut dose-response relationship for lung cancer induction by chronic external gamma irradiation was obtained. Lung cancer induction by cigarette smoking had a linear dependence: smoking of one pack of papiroses (a type of Russian cigarette) per day for 5 y increases the lung cancer risk twofold. The effect was most clearly manifested for squamous-cell carcinoma. 相似文献
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Fatty acids are a major source of fuel for energy production by myocytes. Lipoprotein lipase (LPL) and very low density lipoprotein (VLDL) receptor are abundantly expressed by the heart and skeletal muscles. LPL and possibly VLDL receptor represent the primary route of access to fatty acids contained in circulating triglyceride-rich lipoproteins. Physical exercise and thyroid hormone, which promote energy consumption, upregulate LPL expression in skeletal muscles. This study tested the hypothesis that increased cardiac workload might modulate myocardial LPL and/or VLDL receptor expressions. Accordingly, cardiac tissue LPL activity, LPL and VLDL receptor proteins and mRNA abundance were studied in Sprague-Dawley rats 4 weeks after induction of severe thoracic aorta constriction or sham operation. Elevation of afterload with thoracic aortic constriction led to a significant cardiomegaly and a marked upregulation of cardiac LPL activity, LPL mRNA and LPL protein abundance, but did not modify VLDL receptor mRNA or protein abundance. Thus, increased cardiac workload in this model results in upregulation of myocardial LPL expression which can enhance fatty acid availability to accommodate the heart's increased energy requirement. 相似文献
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H Kino H Inaba N Van De L Van Chau DT Son HT Hao ND Toan LD Cong M Sano 《Canadian Metallurgical Quarterly》1998,29(2):250-254
We present the spatial structure of binase, a small extracellular ribonuclease, derived from 1H-NMR* data in aqueous solution. The total of 20 structures were obtained via torsion angle dynamics using DYANA program with experimental NOE and hydrogen bond distance constraints and phi and chi1 dihedral angle constraints. The final structures were energy minimised with ECEPP/2 potential in FANTOM program. Binase consists of three alpha-helices in N-terminal part (residues 6-16, 26-32 and 41-44), five-stranded antiparallel beta-sheet in C-terminal part (residues 51-55, 70-75, 86-90, 94-99 and 104-108) and two-stranded parallel beta-sheet (residues 22-24 and 49-51). Three loops (residues 36-39, 56-67 and 76-83), which play significant role in biological functioning of binase, are flexible in solution. The differences between binase and barnase spatial structures in solution explain the differences in thermostability of binase, barnase and their hybrids. 相似文献
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The influence of CS-US interval on several different indices of learning in appetitive conditioning.
Four experiments examined the effects of varying the conditioned stimulus-unconditioned stimulus (CS-US) interval (and US density) on learning in an appetitive magazine approach task with rats. Learning was assessed with conditioned response (CR) measures, as well as measures of sensory-specific stimulus-outcome associations (Pavlovian-instrumental transfer, potentiated feeding, and US devaluation). The results from these studies indicate that there exists an inverse relation between CS-US interval and magazine approach CRs, but that sensory-specific stimulus-outcome associations are established over a wide range of relatively long, but not short, CS-US intervals. These data suggest that simple CR measures provide different information about what is learned than measures of the specific stimulus-outcome association, and that time is a more critical variable for the former than latter component of learning. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
39.
QY He AB Mason RC Woodworth BM Tam RT MacGillivray JK Grady ND Chasteen 《Canadian Metallurgical Quarterly》1997,36(48):14853-14860
Human serum transferrin N-lobe (hTF/2N) has four iron-binding ligands, including one histidine, one aspartate, and two tyrosines. The present report elucidates the inequivalence of the two tyrosine ligands (Tyr 95 and Tyr 188) on the metal-binding properties of hTF/2N by means of site-directed mutagenesis, metal release kinetics, and absorption and electron paramagnetic resonance (EPR) spectroscopies. When the liganding tyrosines were mutated individually to phenylalanine, the resulting mutant Y95F showed a weak binding affinity for iron and no affinity for copper, whereas, mutant Y188F completely lost the ability to bind iron but formed a stable complex with copper. Since other studies have demonstrated that mutations of the other two ligands, histidine and aspartate, did not completely abolish iron binding, the present findings suggest that the tyrosine ligand at position 188 is essential for binding of iron to occur. Replacement of Tyr 188 with phenylalanine created a favorable chemical environment for copper coordination but a fatal situation for iron binding. The positions of the two liganding tyrosines in the metal-binding cleft suggest a reason for the inequivalence. 相似文献
40.