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61.
A mixture of D- and L-leucine N-Carboxyanhydride (NCA) having an enantiomeric composition of 65.6% L- and 34.4% D-isomer (i.e. 31.2% enantiomeric excess (e.e)) was polymerized to the extent of 52% with sodium methoxide initiator to yield a polyleucine product the enantiomeric composition of which was 72.7% L- and 27.3% D-leucine (45.4% e.e.). This polymer was in turn partially hydrolyzed by acid, whereupon the unhydrolyzed polyleucine residue was found to have an enantiomeric composition of 77.5% L- and 22.5% D-leucine (55.0% e.e.). Thus the e.e. increase in the partial polymerization step (14.2%) and the partial hydrolysis step (9.6%) combined to total 23.8% for the overall polymerization-hydrolysis sequence. On the basis of these model experiments it is proposed that repetitive partial polymerization hydrolysis reactions, driven by environmental dry-wet cycles, might have been operative on the primitive Earth to engender the abiotic evolution of optically enriched polypeptides. 相似文献
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KM Koslowski PR Shaver JT Casey T Wilson G Yamanaka AK Sheaffer DJ Tenney NE Pederson 《Canadian Metallurgical Quarterly》1999,73(2):1704-1707
Herpes simplex virus (HSV) DNA is cleaved from concatemers and packaged into capsids in infected cell nuclei. This process requires seven viral proteins, including UL15 and UL28. UL15 expressed alone displays a nuclear localization, while UL28 remains cytoplasmic. Coexpression with UL15 enables UL28 to enter nuclei, suggesting an interaction between the two proteins. Additionally, UL28 copurified with UL15 from HSV-infected cells after ion-exchange and DNA affinity chromatography, and the complex sedimented as a 1:1 heterodimer upon sucrose gradient centrifugation. These findings are evidence of a physical interaction of UL15 and UL28 and a functional role for UL15 in directing UL28 to the nucleus. 相似文献
64.
Milton Mcng Liu Moon-Sun Lin Jay M. Chaffln R.R Davison C.J Glover J.A. Bullin 《Petroleum Science and Technology》1997,15(5):471-493
The aging characteristics of naphthene aromatic (NA) and polar aromatic (PA)fractions of an aromatics material were investigated by studying the viscosity and other related properties of aged NA, PA and combined NA and PA fractions of five SHRP core asphalts (AAA-1, AAD-1, AAF-1 相似文献
65.
NE Christensen 《Canadian Metallurgical Quarterly》1986,33(7):5096-5098
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G Wu PK Davis NE Flynn DA Knabe JT Davidson 《Canadian Metallurgical Quarterly》1997,127(12):2342-2349
This study was conducted to determine whether endogenous synthesis of arginine plays a role in regulating arginine homeostasis in postweaning pigs. Pigs were fed a sorghum-based diet containing 0. 98% arginine and were used for studies at 75 d of age (28.4 kg body weight). Mitochondria were prepared from the jejunum and other major tissues for measuring the activities of Delta1-pyrroline-5-carboxylate (P5C) synthase and proline oxidase (enzymes catalyzing P5C synthesis from glutamate and proline, respectively) and of ornithine aminotransferase (OAT) (the enzyme catalyzing the interconversion of P5C into ornithine). For metabolic studies, jejunal enterocytes were incubated at 37 degrees C for 30 min in Krebs-Henseleit bicarbonate buffer containing 2 mmol/L L-glutamine, 2 mmol/L L-[U-14C]proline, and 0-200 micromol/L gabaculine (an inhibitor of OAT). The activities of P5C synthase, proline oxidase and OAT were greatest in enterocytes among all of the tissues studied. Incubation of enterocytes with gabaculine resulted in decreases (P < 0.05) in the synthesis of ornithine and citrulline from glutamine and proline. When gabaculine was orally administered to pigs (0.83 mg/kg body weight) to inhibit intestinal synthesis of citrulline from glutamine and proline, plasma concentrations of citrulline (-26%) and arginine (-22%) decreased (P < 0.05), whereas those of alanine (+21%), ornithine (+17%), proline (+107%), taurine (+56%) and branched-chain amino acids (+21-40%) increased (P < 0.05). On the basis of dietary arginine intake and estimated arginine utilization, the endogenous synthesis of arginine in the 28-kg pig provided >/=50.2% of total daily arginine requirement. Taken together, our results suggest an important role for endogenous synthesis of arginine in regulating arginine homeostasis in postweaning growing pigs, as previously shown in neonatal pigs. 相似文献
68.
NE Crompton 《Canadian Metallurgical Quarterly》1997,53(7):568-575
Telomeres shield the ends of chromosomes from degradation and end-to-end fusions. They shorten at each cell division and when they reach a critically short length, cells arrest in the G1 phase of the cell cycle and undergo senescence. This effectively limits the proliferative potential of cells. Senescence functions as a tumour suppressor mechanism and appears to contribute to the process of ageing. If senescence is circumvented by tumour viruses, proliferation is re-initiated until cells enter crisis. Activation of telomerase prevents telomere attrition and cells become immortal. Cellular response to ionizing radiation involves induction of cell cycle checkpoint arrests and programmed cell death. Because radiation produces double strand breaks in DNA, which cause telomere-less chromosome ends, radiation response appears to be the result of inappropriate induction of cellular senescence mechanisms. 相似文献
69.
Let Y be a continuous, ordinal measure of a latent variable Θ. In general, for factorial designs, an analysis of variance of the observed variable Y cannot be used to draw inferences about main effects and interactions on the latent variable Θ even when the standard normality and equality of variance assumptions hold. If Y is a continuous, ordinal measure of a latent variable Θ; X?,…, Xn are continuous, ordinal measures of latent variables Φ?,…, Φn; and the observed measures have a multivariate normal distribution, then a multiple regression analysis of the observed criterion measure Y and predictors X?,…, Xn can be used to test hypotheses about multivariate associations among the latent variables. Furthermore, the predicted values Y′ are unbiased estimates of quantities that are monotonically related to predicted values on the latent criterion variable Θ. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
70.