Integration framework for intelligent manufacturing processes

In this article, we describe a new approach to applying distributed artificial intelligence techniques to manufacturing processes. The construction of intelligent systems is one of the most important techniques among artificial intelligence research. Our goal is to develop an integrated intelligent system for real time manufacturing processes. An integrated intelligent system is a large knowledge integration environment that consists of several symbolic reasoning systems (expert systems) and numerical computation packages. These software programs are controlled by a meta-system which manages the selection, operation and communication of these programs. A meta-system can be implemented in different language environments and applied to many disciplines. This new architecture can serve as a universal configuration to develop high performance intelligent systems for many complicated industrial applications in real world domains.To whom all correspondence should be addressed.     

Axial vibrations of U-shaped bellows with elastically restrained end conditions

Previous work by Li et al. in the area of axial vibrations of bellows dealt with fixed end conditions. However, it is seen on several occasions that bellow ends are welded to a small pipe spool that has a lumped mass such as a valve or an instrument. Hence, the present paper aims at finding out the effect of elastically restrained ends on the axial natural frequencies. The analysis considers finite stiffness axial restraints on the bellows, i.e. solving the set of equations with non-homogeneous boundary conditions. Two bellow specimens are considered for comparison having the same dimensions as taken by Li in his analysis. The transcendental frequency equation deduced is accurate as the first, second and third mode frequencies computed are in close agreement to the ones obtained by Li.     

Parametric modeling of somatosensory evoked potentials

In this paper, we examine methods of characterizing somatosensory evoked potentials (SEP's) in both the time and frequency domains. We have found that the truncated impulse response (TIR) method produced an accurate time domain model of the SEP signals at model orders greatly reduced from the original state space matrix. The TIR method was valuable for smoothing signals that were slightly corrupted by noise. In this case, the simulated data sequence was close to the original data sequence in the mean squared error sense. For signals that were greatly corrupted by noise, the TIR method was not able to perform as well. Therefore, the TIR method was not a feature extraction method but was valuable for data simulation. In the frequency domain, we have used the autoregressive moving average model (ARMA) to parameterize the SEP signal. An overdetermined set of Yule-Walker equations was created to determine the autoregressive (AR) parameters of the original data with the model order established by the singular value decomposition. From these AR parameters, a residual time series was generated which was used to find the moving average parameters. The resulting ARMA model was used to produce a simulated data sequence. The frequency domain characteristics of the simulated sequence and the corresponding power spectral density of the ARMA filter were very close to the periodogram of the original data sequence. Accurate parameterization was achieved for the SEP waveforms at low filter lengths.     

Effect of pressure gradient on MHD boundary layer over a flat plate

Summary The magnetohydrodynamic (MHD) boundary layer flow over a flat plate is examined here for two cases, viz. a uniform free-stream velocity and a uniform hydrostatic pressure. The nonlinear boundary layer equations are solved using a reliable finite-difference method. The boundary layer physical parameters such as skin-friction coefficient, displacement, momentum and energy thicknesses of the boundary layer are determined. It is found that the normal surface velocity gradient decreases with the local magnetic interaction parameter for the cases of a uniform hydrostatic pressure, whereas in the case of a uniform free-stream volocity it increases with the interaction parameter.     

Intracellular sequestration of sodium by a novel Na+/H+ exchanger in yeast is enhanced by mutations in the plasma membrane H+-ATPase. Insights into mechanisms of sodium tolerance

Sodium tolerance in yeast is disrupted by mutations in calcineurin, a Ca2+/calmodulin-dependent protein phosphatase, which is required for modulation of Na+ uptake and efflux mechanisms. Five Na+-tolerant mutants were isolated by selecting for suppressors of calcineurin mutations, and mapped to the PMA1 gene, encoding the plasma membrane H+-ATPase. One mutant, pma1-alpha4, which has the single amino acid change Glu367 --> Lys at a highly conserved site within the catalytic domain of the ATPase, was analyzed in detail to determine the mechanism of Na+ tolerance. After exposure to Na+ in the culture medium, 22Na influx in the pma1 mutant was reduced 2-fold relative to control, consistent with a similar decrease in ATPase activity. Efflux of 22Na from intact cells was relatively unchanged in the pma1 mutant. However, selective permeabilization of the plasma membrane revealed that mutant cells retained up to 80% of intracellular Na+ within a slowly exchanging pool. We show that NHX1, a novel gene homologous to the mammalian NHE family of Na+/H+ exchangers, is required for Na+ sequestration in yeast and contributes to the Na+-tolerant phenotype of pma1-alpha4.     

Substrate-induced conformational change in a trimeric ornithine transcarbamoylase

The crystal structure of Escherichia coli ornithine transcarbamoylase (OTCase, EC 2.1.3.3) complexed with the bisubstrate analog N-(phosphonacetyl)-L-ornithine (PALO) has been determined at 2.8-A resolution. This research on the structure of a transcarbamoylase catalytic trimer with a substrate analog bound provides new insights into the linkages between substrate binding, protein-protein interactions, and conformational change. The structure was solved by molecular replacement with the Pseudomonas aeruginosa catabolic OTCase catalytic trimer (Villeret, V., Tricot, C., Stalon, V. & Dideberg, O. (1995) Proc. Natl. Acad. Sci. USA 92, 10762-10766; Protein Data Bank reference pdb 1otc) as the model and refined to a crystallographic R value of 21.3%. Each polypeptide chain folds into two domains, a carbamoyl phosphate binding domain and an L-ornithine binding domain. The bound inhibitor interacts with the side chains and/or backbone atoms of Lys-53, Ser-55, Thr-56, Arg-57, Thr-58, Arg-106, His-133, Asn-167, Asp-231, Met-236, Leu-274, Arg-319 as well as Gln-82 and Lys-86 from an adjacent chain. Comparison with the unligated P. aeruginosa catabolic OTCase structure indicates that binding of the substrate analog results in closure of the two domains of each chain. As in E. coli aspartate transcarbamoylase, the 240s loop undergoes the largest conformational change upon substrate binding. The clinical implications for human OTCase deficiency are discussed.     

Structural properties of inorganic materials: tungsten bronze SBN ceramics

Rare-earth doped strontium barium niobates were synthesized using usual ceramic technique. The dopants are La, Ce, Gd, Sm and Nd. The materials were characterized by XRD and density measurements. The grain sizes were determined from SEM analysis. Lattice parameters changed uniformly with rare-earth dopants in unfilled structures. Density measurements and SEM analysis confirmed only minute changes in the densities of the ceramics.